Preparation and optimization of calcium pectate beads for cell encapsulation

2017 ◽  
Vol 135 (2) ◽  
pp. 45685 ◽  
Author(s):  
Shan Zhao ◽  
Ying Zhang ◽  
Yang Liu ◽  
Fan Yang ◽  
Zhilong Xiu ◽  
...  
Keyword(s):  
Cell Encapsulation ◽  
Calcium Pectate

Microbial cell encapsulation as a strategy for the maintenance of stock cultures

LWT ◽  
2019 ◽  
Vol 102 ◽  
pp. 411-417 ◽  
Author(s):  
Joseane C. Bassani ◽  
Vidiany A. Queiroz Santos ◽  
Aneli M. Barbosa-Dekker ◽  
Robert F.H. Dekker ◽  
Mário Antônio A. da Cunha ◽  
...  
Keyword(s):  
Cell Encapsulation ◽  
Microbial Cell

scholarly journals Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules

2020 ◽  
Vol 10 (2) ◽  
pp. 290-296
Author(s):  
Fariba Hajifathaliha ◽  
Arash Mahboubi ◽  
Elham Mohit ◽  
Noushin Bolourchian ◽  
Vahid Khalaj ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Survival ◽  
Metabolic Activity ◽  
Encapsulation Efficiency ◽  
Cell Encapsulation ◽  
High Price ◽  
Ethylene Imine ◽  
Linear Poly ◽  
Cellular Metabolic Activity ◽  
Poly Ethylene

Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear poly ethylene imine (LPEI), thanks to its highly similar structure to PLL, could be considered as a proper cost-effective alternative. In this study LPEI and PLL were compared as covering layers of cell-loaded alginate-LPEI-alginate (cALA) and alginate-PLL-alginate (cAPA) microcapsules. Methods: In addition to the physico-mechanical properties, the encapsulation efficiency, cell survival post encapsulation, cell viability, and cellular metabolic activity within the microcapsules were evaluated using trypan blue, live/dead cell staining, and MTT test, respectively. Results: Physico-mechanical evaluation of the microcapsules revealed that the cell microencapsulation process did not affect their shape, size, and mechanical stability. Although the encapsulation efficiency for cALA and cAPA was not different (P>0.05), cell survival post encapsulation was higher in cALA than in cAPA (P<0.05) which could be the reason for the higher cell viability and also cellular metabolic activity within these microcapsules in comparison to cAPA. Conclusion: Here, based on these results, ALA could be introduced as a preferable alternative to APA for cell encapsulation.

scholarly journals Transparent PEG-Fibrin Gel as a Flexible Tool for Cell Encapsulation

2018 ◽  
Vol 10 (1) ◽  
pp. 64 ◽  
Author(s):  
A.I. Shpichka ◽  
V.A. Revkova ◽  
N.A. Aksenova ◽  
G.M. Yusubalieva ◽  
V.A. Kalsin ◽  
...  
Keyword(s):  
Cell Encapsulation ◽  
Fibrin Gel ◽  
Flexible Tool
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