ChemInform Abstract: 9-(Difluorophosphonoalkyl)guanines as a New Class of Multisubstrate Analogue Inhibitors of Purine Nucleoside Phosphorylase.

ChemInform ◽  
2010 ◽  
Vol 22 (15) ◽  
pp. no-no
Author(s):  
S. HALAZY ◽  
A. EHRHARD ◽  
C. DANZIN
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase ◽  
New Class

scholarly journals Crystal structures of a new class of pyrimidine/purine nucleoside phosphorylase (ppnP) revealed a Cupin fold

2021 ◽  
Author(s):  
Yan Wen ◽  
Xiaojia Li ◽  
Wenting Guo ◽  
Baixing Wu
Keyword(s):  
Crystal Structures ◽  
Purine Nucleoside Phosphorylase ◽  
Binding Pocket ◽  
Purine Nucleoside ◽  
Structural Basis ◽  
Nucleoside Phosphorylase ◽  
E Coli ◽  
New Class ◽  
Nucleoside Phosphorylases ◽  
Pocket Formation

Nucleotides metabolism is a fundamental process in all organisms. Two families of nucleoside phosphorylases (NP) that catalyze the phosphorolytic cleavage of the glycosidic bond in nucleosides have been found, including the trimeric or hexameric NP-I and dimeric NP-II family enzymes. Recently studies revealed another class of NP protein in E. coli named Pyrimidine/purine nucleoside phosphorylase (ppnP), which can catalyze the phosphorolysis of diverse nucleosides and yield D-ribose 1-phosphate and the respective free bases. Here, we solve the crystal structures of ppnP from E. coli and the other three species. Our studies revealed that the structure of ppnP belongs to the Rlmc-like cupin fold and showed as a rigid dimeric conformation. Detail analysis revealed a potential nucleoside binding pocket full of hydrophobic residues. And the residues involved in the dimer and pocket formation are all well conserved in bacteria. Since the cupin fold is a large superfamily in the biosynthesis of natural products, our studies provide the structural basis for understanding and the directed evolution of NP proteins.

N-Phosphonocarbonylpyrrolidine Derivatives of Guanine: A New Class of Bi-Substrate Inhibitors of Human Purine Nucleoside Phosphorylase

2012 ◽  
Vol 55 (4) ◽  
pp. 1612-1621 ◽  
Author(s):  
Dominik Rejman ◽  
Natalya Panova ◽  
Pavel Klener ◽  
Bokang Maswabi ◽  
Radek Pohl ◽  
...  
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase ◽  
New Class ◽  
Derivatives Of
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