Abstract
Genetic variants of the COVID-19 causative virus have been arising and circulating globally. In many countries especially in developing ones with a huge population, vaccination has become one of the major challenges. SARS-CoV-2 variants’ fast transmission rate has upsurge the COVID cases, leading to more stress on health systems. In the current COVID-19 scenario, there is the requirement of more adequate diagnostic approaches to check the COVID-19 spread. Out of many diagnostic approaches, a magnetic nanoparticle-based reverse transcription-polymerase chain reaction could be nontrivial. The use of magnetic nanoparticles to separate nucleic acid of SARS-CoV-2 from the patient samples and applied for detection is an easy and more effective way for COVID-19 patient detection. Herein, the magnetic nanoparticles are synthesized using the sol-gel autocombustion methods and then, successfully coated with biopolymer (chitosan) using ultra-sonication. Chitosan-coated nanoparticles are successfully integrated into the graphene oxide sheets to introduce carboxyl groups. Crystallite size calculation, morphological and magnetic studies of synthesized magnetic nanoparticles, and multifunctional magnetic nanoparticles are done using XRD, SEM, TEM, and VSM respectively. Besides the potentiality of the fabricated nanocomposites in RNA extraction protocol is also discussed with schematic representation.
Detection of FGFR2 : FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide-loaded Magnetic Nanoparticles
