This study examines experimentally the influence of limiting factors on seed to ovule ratio and seed weight in three alpine populations of the perennial herb Ranunculus acris L. at Finse, southwest Norway. To test for pollen and resource limitation, seed production of supplementally pollinated or completely defoliated plants was compared to that of untreated plants. In one population the experiment was repeated twice during the season. In mid-season, supplemental hand-pollination had no significant effect on seed to ovule ratio, but significantly increased the seed weight, whereas complete defoliation resulted in a significant decrease in seed to ovule ratio and a marginally significant decrease in seed weight. These results indicate that pollen limitation occurred on seed weight and resource limitation mainly on seed to ovule ratio. Seed production in late-flowering control and defoliated plants in one population was nearly completely absent, whereas supplemental pollination increased seed production substantially. Most seeds were produced at the lowest elevation, and frost damage on developing seeds was most pronounced at the highest elevation population, suggesting that severe weather conditions at the highest altitudes had a negative influence on reproductive processes. Significant positive relationships between seed number and seed weight were found, possibly reflecting a large among-plant variation in total resource availability. Key words: altitude gradient, climate, defoliation, pollen and resource availability, seasonal variation, seed size.