Osteoblast culture on bioerodible polymers: studies of initial cell adhesion and spread

1992 ◽  
Vol 3 (6) ◽  
pp. 359-364 ◽  
Author(s):  
Cato T. Laurencin ◽  
Carol D. Morris ◽  
Henri Pierre-Jacques ◽  
Edith R. Schwartz ◽  
Altorous R. Keaton ◽  
...  
2007 ◽  
Vol 361-363 ◽  
pp. 1115-1118
Author(s):  
Un Hye Kwon ◽  
Jung Suk Han ◽  
In Young Ryu ◽  
Dae Joon Kim

The initial osteoblast like cell response to bioactive nano-sized hydroxyapatite (HAp) and bioinert zirconia was evaluated with the cell morphology by SEM and cell adhesion proteins by fluorescence microscopy. Surface roughness also measured by a confocal laser microscopy. The surface roughness and topography was almost identical among specimens. The nano-sized HAp specimens showed better initial cell adhesion and activity than bioinert zirconia ceramics.


Soft Matter ◽  
2016 ◽  
Vol 12 (1) ◽  
pp. 272-280 ◽  
Author(s):  
Christina Müller ◽  
Tilo Pompe

Elastic and viscous substrate properties distinctively determine traction force evolution in early and late stages of the initial cell adhesion process.


2005 ◽  
Vol 2005.6 (0) ◽  
pp. 117-118
Author(s):  
Shunsuke SEMIYA ◽  
Satoshi TERAMURA ◽  
Koji YAMAMOTO ◽  
Naoya HATANO ◽  
Naohide TOMITA

2008 ◽  
Vol 95 (11) ◽  
pp. 5424-5431 ◽  
Author(s):  
Christine Selhuber-Unkel ◽  
Mónica López-García ◽  
Horst Kessler ◽  
Joachim P. Spatz

2017 ◽  
Vol 148 (3) ◽  
pp. 273-288 ◽  
Author(s):  
Martina Verdanova ◽  
Pavla Sauerova ◽  
Ute Hempel ◽  
Marie Hubalek Kalbacova

Author(s):  
Zeinab Rezaei Esfahrood ◽  
Amirhosein Zamanian ◽  
Maryam Torshabi ◽  
Maryam Abrishami

AbstractDifferent compounds of smoking (e.g., nicotine and cotinine) are risk factors for various diseases such as oral cancer and periodontal diseases. Some studies reported the negative effects of nicotine on cell proliferation and differentiation. The present in vitro study assessed the effects of nicotine and cotinine (long-acting metabolite of nicotine) on the attachment and viability of human gingival fibroblast (HGF) cells to tooth root surfaces.A total of 70 teeth specimens were placed into 48-well culture plates and covered with HGF cell suspension, in complete Dulbecco’s modified Eagle’s medium culture medium containing 1 nM, 1 μm, 1 mM, and 5 mM of nicotine and cotinine concentrations. Cellular attachment and viability measured using an MTT assay and a scanning electron microscope were used for cell morphological evaluation.After 24 h, low (nanomolar and micromolar) and high concentrations (millimolar) of nicotine and cotinine caused a significant reduction in the initial cell adhesion in comparison with the control group, but no significant difference was observed between the nicotine and the cotinine groups (p<0.05). Dentally attached cells with low concentrations of nicotine and cotinine proliferated 48 h after exposure, the same as the control group. However, dentally attached cells with high concentrations of nicotine and cotinine (especially 5 mM) did not proliferate 24 h after exposure (p<0.05).Low concentrations of nicotine and cotinine caused a reduction in the initial cell adhesion. However, no significant adverse effects on the proliferation of attached cells were seen in the longer period. High concentrations of nicotine and cotinine have adverse effects on the cell adhesion and proliferation of HGF cells.


2021 ◽  
Vol 199 ◽  
pp. 109402
Author(s):  
Na Li ◽  
Yonggang Wang ◽  
Dan Zhao ◽  
Bo Deng ◽  
Xialian Fan ◽  
...  

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