The effect of nicotine and cotinine on human gingival fibroblasts attachment to root surfaces

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Zeinab Rezaei Esfahrood ◽  
Amirhosein Zamanian ◽  
Maryam Torshabi ◽  
Maryam Abrishami
Keyword(s):  
Cell Adhesion ◽  
Adverse Effects ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Initial Cell ◽  
Low Concentrations ◽  
High Concentrations ◽  
Root Surfaces ◽  
Initial Cell Adhesion

AbstractDifferent compounds of smoking (e.g., nicotine and cotinine) are risk factors for various diseases such as oral cancer and periodontal diseases. Some studies reported the negative effects of nicotine on cell proliferation and differentiation. The present in vitro study assessed the effects of nicotine and cotinine (long-acting metabolite of nicotine) on the attachment and viability of human gingival fibroblast (HGF) cells to tooth root surfaces.A total of 70 teeth specimens were placed into 48-well culture plates and covered with HGF cell suspension, in complete Dulbecco’s modified Eagle’s medium culture medium containing 1 nM, 1 μm, 1 mM, and 5 mM of nicotine and cotinine concentrations. Cellular attachment and viability measured using an MTT assay and a scanning electron microscope were used for cell morphological evaluation.After 24 h, low (nanomolar and micromolar) and high concentrations (millimolar) of nicotine and cotinine caused a significant reduction in the initial cell adhesion in comparison with the control group, but no significant difference was observed between the nicotine and the cotinine groups (p<0.05). Dentally attached cells with low concentrations of nicotine and cotinine proliferated 48 h after exposure, the same as the control group. However, dentally attached cells with high concentrations of nicotine and cotinine (especially 5 mM) did not proliferate 24 h after exposure (p<0.05).Low concentrations of nicotine and cotinine caused a reduction in the initial cell adhesion. However, no significant adverse effects on the proliferation of attached cells were seen in the longer period. High concentrations of nicotine and cotinine have adverse effects on the cell adhesion and proliferation of HGF cells.

scholarly journals Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chu-nan Zhang ◽  
Lin-yi Zhou ◽  
Shu-jiao Qian ◽  
Ying-xin Gu ◽  
Jun-yu Shi ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Underlying Mechanism ◽  
Cell Number ◽  
Human Gingival Fibroblasts ◽  
Superior Performance ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Integrin Β1

Abstract Objectives This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms. Materials and methods Titanium plates were divided into two groups: (1) no coating (Ti, control), (2) Tantalum-coated titanium (Ta-coated Ti). All samples were characterized by scanning electronic microscopy, surface roughness, and hydrophilicity. Fibroblasts’ performance were analyzed by attached cell number at 1 h, 4 h, and 24 h, morphology at 1 h and 4 h, viability at 1 day, 3 days, 5 days, and 7 days, recovery after wounding at 6 h, 12 h, and 24 h. RT-PCR, western blot were applied to detect attachment-related genes’ expression and protein synthesis at 4 h and 24 h. Student’s t test was used for statistical analysis. Results Tantalum-coated titanium demonstrates a layer of homogeneously distributed nano-grains with mean diameter of 25.98 (± 14.75) nm. It was found that after tantalum deposition, human gingival fibroblasts (HGFs) adhesion, viability, proliferation, and migration were promoted in comparison to the control group. An upregulated level of Integrin β1 and FAK signaling was also detected, which might be the underlying mechanism. Conclusion In the present study, adhesion, viability, proliferation, migration of human gingival fibroblasts are promoted on tantalum-coated titanium, upregulated integrin β1 and FAK might contribute to its superior performance, indicating tantalum coating can be applied in transmucosal part of dental implant. Clinical significance Tantalum deposition on titanium surfaces can promote human gingival fibroblast adhesion, accordingly forming a well-organized soft tissue sealing and may contribute to a successful osseointegration.

Initial Attachment of MC3T3-E1 Cells on Nano-HAp Surfaces

2007 ◽  
Vol 361-363 ◽  
pp. 1115-1118
Author(s):  
Un Hye Kwon ◽  
Jung Suk Han ◽  
In Young Ryu ◽  
Dae Joon Kim
Keyword(s):  
Surface Roughness ◽  
Cell Adhesion ◽  
Fluorescence Microscopy ◽  
Cell Morphology ◽  
Confocal Laser ◽  
Initial Attachment ◽  
Zirconia Ceramics ◽  
Initial Cell ◽  
Cell Adhesion Proteins ◽  
Initial Cell Adhesion

The initial osteoblast like cell response to bioactive nano-sized hydroxyapatite (HAp) and bioinert zirconia was evaluated with the cell morphology by SEM and cell adhesion proteins by fluorescence microscopy. Surface roughness also measured by a confocal laser microscopy. The surface roughness and topography was almost identical among specimens. The nano-sized HAp specimens showed better initial cell adhesion and activity than bioinert zirconia ceramics.

scholarly journals The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin–Phycocyanin Mixture on Enterococcus faecalis: The Influence of Different Light Sources

2020 ◽  
Vol 10 (12) ◽  
pp. 4290 ◽  
Author(s):  
Nasim Chiniforush ◽  
Maryam Pourhajibagher ◽  
Steven Parker ◽  
Stefano Benedicenti ◽  
Abbas Bahador ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Light Sources ◽  
Gingival Fibroblast ◽  
Antimicrobial Photodynamic Therapy ◽  
Cell Viability Assay ◽  
Vitro Effect

The purpose of this study was to evaluate the in vitro effect of the chlorophyllin–phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin–phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 ± 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420–480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin–phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity.

scholarly journals Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Soft Matter ◽  
2016 ◽  
Vol 12 (1) ◽  
pp. 272-280 ◽  
Author(s):  
Christina Müller ◽  
Tilo Pompe
Keyword(s):  
Cell Adhesion ◽  
Traction Force ◽  
Initial Cell ◽  
Ligand Affinity ◽  
Substrate Properties ◽  
Substrate Elasticity ◽  
Late Stages ◽  
Initial Cell Adhesion ◽  
Adhesion Process

Elastic and viscous substrate properties distinctively determine traction force evolution in early and late stages of the initial cell adhesion process.

NH4Cl affects the expression of Wnt/β-catenin pathway in hepatocytes

2018 ◽  
Vol 96 (3) ◽  
pp. 281-286
Author(s):  
Lu Bai ◽  
Jingjing Li ◽  
Xiaorui Liu ◽  
Shasha Li ◽  
Fulei Li ◽  
...  
Keyword(s):  
Protein Expression ◽  
Control Group ◽  
Cyclin D ◽  
Chang Liver Cell ◽  
Expression Levels ◽  
Low Concentrations ◽  
Short Period ◽  
Mrna And Protein Expression ◽  
High Concentrations ◽  
Chang Liver

We intended to explore whether NH4Cl influences the viability and regulates the expression of Wnt/β-catenin pathway in hepatocytes. The Chang liver cell line was used and cultured with different concentrations of NH4Cl (2.5, 5, 10, 20, 40, and 50 mmol/L) for 12, 24, and 48 h. The viability of hepatocytes was detected by MTT assay. The mRNA and protein expression level was analyzed with qRT–PCR and Western blotting, respectively. NH4Cl concentration significantly affects the viability of hepatocytes. With the increase of NH4Cl concentration, the viability of hepatocytes was decreased, accordingly. The mRNA and protein expression of Wnt1, β-catenin, and cyclin D was significantly increased after treatment with low concentrations of NH4Cl as compared with the control group, whereas their expression levels were decreased after treatment with high concentrations of NH4Cl. The mRNA and protein expression of Wnt1, β-catenin, and cyclin D was also significantly increased after treatment with NH4Cl for a short period as compared with the control group, whereas their expression levels were decreased after treatment with NH4Cl for a long period. In addition, we found NH4Cl treatment significantly reversed the results after RNA silencing of Wnt1 in hepatocytes. NH4Cl influences the viability of hepatocytes and affects the expression of Wnt/β-catenin pathway in hepatocytes.

1253 Effect of Centrifugal Condition on Initial Cell Adhesion

2005 ◽  
Vol 2005.6 (0) ◽  
pp. 117-118
Author(s):  
Shunsuke SEMIYA ◽  
Satoshi TERAMURA ◽  
Koji YAMAMOTO ◽  
Naoya HATANO ◽  
Naohide TOMITA
Keyword(s):  
Cell Adhesion ◽  
Initial Cell ◽  
Initial Cell Adhesion

Osteoblast culture on bioerodible polymers: studies of initial cell adhesion and spread

1992 ◽  
Vol 3 (6) ◽  
pp. 359-364 ◽  
Author(s):  
Cato T. Laurencin ◽  
Carol D. Morris ◽  
Henri Pierre-Jacques ◽  
Edith R. Schwartz ◽  
Altorous R. Keaton ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Initial Cell ◽  
Osteoblast Culture ◽  
Initial Cell Adhesion

scholarly journals Effects of Microplastics on the Feeding Rates of Larvae of a Coastal Fish: Direct Consumption, Trophic Transfer, and Effects on Growth and Survival

2021 ◽  
Author(s):  
Christine Uy ◽  
Darren Johnson
Keyword(s):  
Larval Fish ◽  
Trophic Transfer ◽  
Control Group ◽  
Feeding Rates ◽  
Lower Growth ◽  
Larval Phase ◽  
Coastal Fish ◽  
Growth And Survival ◽  
Low Concentrations ◽  
High Concentrations

Abstract Microplastics are now found throughout the world’s oceans, and although many organisms ingest microplastics, less is known about how plastics in seawater may affect key processes such as feeding rate, growth, and survival. We used a series of laboratory experiments to test whether microplastics in seawater affected the feeding rates of larvae of the California Grunion, Leuresthes tenuis. In addition, we tested whether trophic transfer of microplastics from zooplankton to larval fish can occur and affect growth and survival of fish. We measured feeding rates of grunion larvae at various concentrations of 75–90 µm and 125–250 µm polyethylene microplastics and under both still water and turbulent conditions. In these experiments, exposure to microplastics had modest effects on feeding rates, though responses may be somewhat complex. Low concentrations of microplastics increased feeding rates compared to the control, but at higher concentrations, feeding rates were indistinguishable from those in the control group though effects were small compared to natural variation in feeding rates among individual fish. Experiments to test for trophic transfer of microplastics revealed that grunion larvae that were fed brine shrimp exposed to high concentrations of microplastics had lower growth rates and elevated mortality rates. Overall, our results suggest that the direct effects of microplastics on feeding rates of California Grunion during the early larval phase are minor, while the trophic transfer of microplastics from zooplankton to larval fish may have significant effects on their growth and survival.

scholarly journals Effect of Concentrated growth factor (CGF) on human gingival fibroblast viability on titanium discs

2021 ◽  
Author(s):  
Masoumeh Faramarzi ◽  
Leila Roshangar ◽  
Adileh Shirmohammadi ◽  
Mehrnoosh Sadighi ◽  
Azadeh Madanipour ◽  
...  
Keyword(s):  
Growth Factor ◽  
Critical Role ◽  
Control Group ◽  
P Value ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Term Survival ◽  
Accelerate Wound Healing

Abstract Objective Attachment of peri- implant mucosa to implant is crucial for long term survival of implant. Soft tissue healing around implants has been of great interest due to its important role in long-term maintenance of implant therapy. Considering the critical role of gingival fibroblasts in periodontal repair, the aim of this study was to evaluate the Concentrated Growth Factor (CGF) as an innovative approach to accelerate wound healing and increase the connective tissue seal around dental implants. Results 40% and 80% Concentrations of CGF significantly improved human gingival fibroblasts (HGF) viability compared to the control group (P value = 0.001). But the comparison of the other group with the control group was not statistically significant. The difference between 40% and 80% concentrations of CGF was not statistically significant (P value = 0.061). Results showed that the viability of HGF treated with CGF on titanium discs(test groups 2) significantly increased as compared to the test groups 1(without CGF) at 24 hours (P value = 0.001).Our results showed that 40% concentration of CGF at 24 hours significantly increased HGF viability.

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