Quantitative Estimation of Nitrogenase Activity: Acetylene Reduction Assay

2020 ◽  
pp. 37-39
Author(s):  
M. Senthilkumar ◽  
N. Amaresan ◽  
A. Sankaranarayanan
Keyword(s):  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Quantitative Estimation ◽  
Acetylene Reduction Assay ◽  
Reduction Assay

Interactions between Lugol's fixative and ethylene in the acetylene-reduction assay for nitrogenase activity in lake water

1980 ◽  
Vol 26 (5) ◽  
pp. 599-605 ◽  
Author(s):  
Lars Leonardson
Keyword(s):  
Liquid Phase ◽  
Vapor Phase ◽  
Lake Water ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Volume Ratio ◽  
Aquatic Organisms ◽  
Acetylene Reduction Assay ◽  
Reduction Assay ◽  
Phase Loss

Lugol's solution is a practical and efficient fixative for the acetylene-reduction assay of nitrogenase activity in aquatic organisms. Correction must be made, however, for the solubility of ethylene in the liquid phase and reactions between Lugol's solution and ethylene. With a vapor phase – liquid phase volume ratio of 1.9:1, the mean solubility of ethylene in mixtures of lake water and Lugol's solution was 7.2%. No correlation was found between ethylene solubility and the concentration of Lugol's solution. Storage of fixed samples for more than 1 day before gas chromatographic analysis resulted in increased loss of ethylene from the vapor phase; the loss amounted to ca. 18% after 3 days. Higher losses were noted at higher concentrations of Lugol's solution. Most probably these effects were caused by iodine addition to ethylene, as indicated by the consumption of ethylene by iodine – potassium iodide solutions. The reaction was catalyzed by the rubber septa of the incubation vessels when the septa were in contact with the liquid phase. Loss of ethylene decreased with increased concentration of phytoplankton because the organisms absorbed iodine. By using a standardized technique and determining ethylene solubility and reaction patterns between ethylene and the mixture of water and Lugol's solution, it is possible to correct for the loss of ethylene.

Bacterial dinitrogen fixation in the leaf of the northern pitcher plant (Sarracenia purpurea)

1991 ◽  
Vol 69 (10) ◽  
pp. 2296-2298 ◽  
Author(s):  
Andrew B. Prankevicius ◽  
Duncan M. Cameron
Keyword(s):  
Key Words ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Dinitrogen Fixation ◽  
Acetylene Reduction Assay ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Physiological Conditions ◽  
Reduction Assay ◽  
Unidentified Source

Bacterial (Azotobacteriaceae) nitrogenase activity in the leaf cavity of the insectivorous northern pitcher plant (Sarracenia purpurea L.) was assessed by acetylene reduction assay. Results indicated that bacterial dinitrogen fixation occurs in the leaf cavity under normal physiological conditions. This may represent a previously unidentified source of nitrogen for the pitcher plant, as the level of nitrogenase activity measured was sufficient to provide the plant with nitrogen well in excess of that required. Key words: pitcher plant, Sarracenia purpurea, dinitrogen fixation, Azotobacteriaceae, nitrogenase.

Sign in / Sign up

Export Citation Format

Share Document