3D Tissue Explant and Single-Cell Suspension Organoid Culture Systems for Ex Vivo Drug Testing on Human Tonsil-Derived T Follicular Helper Cells

2021 ◽  
pp. 267-288
Author(s):  
Angelika Schmidt ◽  
Dirk Baumjohann
Keyword(s):  
Cell Suspension ◽  
Single Cell ◽  
Drug Testing ◽  
Ex Vivo ◽  
T Follicular Helper Cells ◽  
Single Cell Suspension ◽  
Human Tonsil ◽  
Culture Systems ◽  
Organoid Culture ◽  
Follicular Helper

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

Preparation of Single Cell Suspension from Human Lung Tissue v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo ◽  
Basak Ural ◽  
Maya M.L. Poon
Keyword(s):  
Epithelial Cells ◽  
Cell Suspension ◽  
Single Cell ◽  
Lung Tissue ◽  
Immune Cells ◽  
Human Lung ◽  
Dilution Factor ◽  
Single Cell Suspension ◽  
Human Lung Tissue ◽  
Defined Media

This protocol describes a method for the isolation of the immune cells, structural and epithelial cells, and progenitors from human lung sections of about two grams. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

scholarly journals An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae

MethodsX ◽  
2018 ◽  
Vol 5 ◽  
pp. 1287-1290 ◽  
Author(s):  
Erica Bresciani ◽  
Elizabeth Broadbridge ◽  
Paul P. Liu
Keyword(s):  
Cell Suspension ◽  
Single Cell ◽  
Zebrafish Embryos ◽  
Single Cell Suspension

HTAPP_Enrichment of EpCAM+ cells from single-cell suspension for single-cell RNA-seq v1 (protocols.io.bucfnstn)

protocols.io ◽  
2021 ◽  
Author(s):  
Isaac Wakiro ◽  
Sara Napolitano ◽  
Sébastien Vigneau ◽  
Asaf Rotem ◽  
Orit Rozenblatt-Rosen ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Single Cell ◽  
Rna Seq ◽  
Single Cell Suspension

Chronic rhinosinusitis: association of recalcitrant nasal polyposis and fungal finding in polyp’s single-cell suspension

2015 ◽  
Vol 272 (12) ◽  
pp. 3727-3734 ◽  
Author(s):  
Aleksandra Barac ◽  
Marina Pekmezovic ◽  
Vesna Tomic Spiric ◽  
Aleksandar Trivic ◽  
Jelena Marinkovic ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Single Cell ◽  
Chronic Rhinosinusitis ◽  
Nasal Polyposis ◽  
Single Cell Suspension
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