The Regulation of Prostaglandin E2 Synthesis by Interleukin-1β and Ceramide in Human Fibroblasts: Effects on Cyclooxygenase-1, Cyclooxygenase-2, and Phospholipase A2 Gene Expression

1996 ◽  
pp. 111-117
Author(s):  
Leslie R. Ballou ◽  
Kanyawim Kirtikara ◽  
Stanley J. F. Laulederkind
Keyword(s):  
Gene Expression ◽  
Prostaglandin E2 ◽  
Phospholipase A2 ◽  
Human Fibroblasts ◽  
Cyclooxygenase 2 ◽  
Interleukin 1Β ◽  
Cyclooxygenase 1

scholarly journals Basic calcium phosphate crystal-induced prostaglandin E2 production in human fibroblasts: Role of cyclooxygenase 1, cyclooxygenase 2, and interleukin-1?

2004 ◽  
Vol 50 (5) ◽  
pp. 1642-1649 ◽  
Author(s):  
Maria P. Morgan ◽  
Linda C. Whelan ◽  
John D. Sallis ◽  
Conor J. McCarthy ◽  
Desmond J. Fitzgerald ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Prostaglandin E2 ◽  
Interleukin 1 ◽  
Human Fibroblasts ◽  
Cyclooxygenase 2 ◽  
Basic Calcium Phosphate ◽  
Cyclooxygenase 1 ◽  
Calcium Phosphate Crystal ◽  
Basic Calcium Phosphate Crystal

INTERFERON-γ AND INTERLEUKIN 4 INHIBIT INTERLEUKIN 1β-INDUCED DELAYED PROSTAGLANDIN E2GENERATION THROUGH SUPPRESSION OF CYCLOOXYGENASE-2 EXPRESSION IN HUMAN FIBROBLASTS

Cytokine ◽  
2000 ◽  
Vol 12 (6) ◽  
pp. 603-612 ◽  
Author(s):  
Yoshinori Hayashi ◽  
Makoto Kobayashi ◽  
Hiroshi Kuwata ◽  
Gen-ichi Atsumi ◽  
Katsutoshi Deguchi ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
Interferon Γ ◽  
Cyclooxygenase 2 ◽  
Interleukin 1Β ◽  
Interleukin 4

scholarly journals Leishmania donovani-Induced Prostaglandin E2 Generation Is Critically Dependent on Host Toll-Like Receptor 2–Cytosolic Phospholipase A2 Signaling

2016 ◽  
Vol 84 (10) ◽  
pp. 2963-2973 ◽  
Author(s):  
Amrita Bhattacharjee ◽  
Saikat Majumder ◽  
Shibali Das ◽  
Sweta Ghosh ◽  
Satabdi Biswas ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Phospholipase A2 ◽  
Leishmania Donovani ◽  
Cytosolic Phospholipase A2 ◽  
Cyclooxygenase 2 ◽  
Toll Like Receptor ◽  
Content Type ◽  
Cytosolic Phospholipase ◽  
Pge2 Release ◽  
Toll Like Receptor 2

Visceral leishmaniasis (VL) is the second-largest parasitic killer disease after malaria. During VL, the protozoanLeishmania donovaniinduces prostaglandin E2 (PGE2) generation within host macrophages to aid parasite survival. PGE2 significantly influences leishmanial pathogenesis, asL. donovaniproliferation is known to be attenuated in PGE2-inhibited macrophages. Here, we report for the first time that signaling via macrophage Toll-like receptor 2 (TLR2) plays an instrumental role in inducing PGE2 release fromL. donovani-infected macrophages. This signaling cascade, mediated via the TLR2–phosphatidylinositol 3-kinase (PI3K)–phospholipase C (PLC) signaling pathway, was found to be indispensable for activation of two major enzymes required for PGE2 generation: cytosolic phospholipase A2 (cPLA2) and cyclooxygenase 2 (Cox2). Inhibition of cPLA2, but not secreted phospholipase A2 (sPLA2) or calcium-independent phospholipase A2 (iPLA2), arrestedL. donovaniinfection. During infection, cPLA2 activity increased >7-fold in a calcium-dependent and extracellular signal-regulated kinase (ERK)-dependent manner, indicating that elevation of intracellular calcium and ERK-mediated phosphorylation was necessary forL. donovani-induced cPLA2 activation. For transcriptional upregulation of cyclooxygenase 2, activation of the calcium-calcineurin-nuclear factor of activated T cells (NFAT) signaling was required in addition to the TLR2-PI3K-PLC pathway. Detailed studies by site-directed mutagenesis of potential NFAT binding sites and chromatin immunoprecipitation (ChIP) analysis revealed that the binding of macrophage NFATc2, at the −73/−77 site on thecox2promoter, inducedL. donovani-drivencox2transcriptional activation. Collectively, these findings highlight the contribution of TLR2 downstream signaling toward activation of cPLA2 and Cox2 and illustrate how the TLR2-PI3K-PLC pathway acts in a concerted manner with calcium-calcineurin-NFATc2 signaling to modulate PGE2 release fromL. donovani-infected macrophages.

Prostaglandin E2 production in ovarian cancer cell lines is regulated by cyclooxygenase-1, not cyclooxygenase-2

2005 ◽  
Vol 73 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Yuko Kino ◽  
Fumiaki Kojima ◽  
Kazushige Kiguchi ◽  
Rie Igarashi ◽  
Bunpei Ishizuka ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Prostaglandin E2 ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Cancer Cell Lines ◽  
Cyclooxygenase 2 ◽  
Ovarian Cancer Cell Lines ◽  
Cyclooxygenase 1

scholarly journals Metallic but not ceramic wear particles increase prostaglandin E2 release and interleukin-1β gene expression in human blood monocytes in vitro

2006 ◽  
Author(s):  
Marco Galli ◽  
Caterina Toriani Terenzi ◽  
Gianluca Falcone ◽  
Ubaldo Pozzetto ◽  
Giuseppe Tringali ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostaglandin E2 ◽  
Human Blood ◽  
Interleukin 1Β ◽  
Wear Particles ◽  
Blood Monocytes

Inhibition of cyclooxygenase-2, but not cyclooxygenase-1, reduces prostaglandin E2 secretion from diabetic rat retinas

2004 ◽  
Vol 498 (1-3) ◽  
pp. 275-278 ◽  
Author(s):  
Surya P. Ayalasomayajula ◽  
Aniruddha C. Amrite ◽  
Uday B. Kompella
Keyword(s):  
Prostaglandin E2 ◽  
Cyclooxygenase 2 ◽  
Diabetic Rat ◽  
Cyclooxygenase 1
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