Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research

2019 ◽  
pp. 145-151
Author(s):  
Naoi Hosoe ◽  
Takuya Keima ◽  
Yuji Fujimoto ◽  
Yuka Hagiwara-Komoda ◽  
Masayoshi Hashimoto ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Virus ◽  
Virus Research

scholarly journals Biosecurity Implications of New Technology and Discovery in Plant Virus Research

2013 ◽  
Vol 9 (8) ◽  
pp. e1003337 ◽  
Author(s):  
Robin MacDiarmid ◽  
Brendan Rodoni ◽  
Ulrich Melcher ◽  
Francisco Ochoa-Corona ◽  
Marilyn Roossinck
Keyword(s):  
Plant Virus ◽  
New Technology ◽  
Virus Research

scholarly journals Plant virus evolution under strong drought conditions results in a transition from parasitism to mutualism

2020 ◽  
Author(s):  
Rubén González ◽  
Anamarija Butkovic ◽  
Francisco Escaray ◽  
Javier Martínez-Latorre ◽  
Ízan Melero ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Plant Virus ◽  
Virus Evolution ◽  
Drought Conditions ◽  
Effects Of Drought ◽  
Plant Virus Evolution ◽  
Virus Adaptation ◽  
Host Genes

Abstract Environmental conditions are an important factor driving pathogens evolution. Here we explore the effects of drought stress in plant virus evolution. We evolved a potyvirus in well-watered and drought conditions in Arabidopsis thaliana accessions that differ in their response to virus infection. Virus adaptation occurred in all accessions independently of watering status. Drought-evolved viruses conferred a significantly higher tolerance to drought to infected plants. By contrast, non-significant increases in tolerance were observed in plants infected with viruses evolved under standard watering. The magnitude of this effect was dependent on the plant accessions. Differences in tolerance were correlated to alterations in the expression of host genes, some involved in regulation of the circadian clock, as well as in deep changes in the balance of phytohormones regulating defense and growth signaling pathways. Our results show that viruses can promote host survival in situations of abiotic stress, being the magnitude of such benefit a selectable trait.

Insect cell culture as a tool in plant virus research: a historical overview

2020 ◽  
Vol 48 (2) ◽  
pp. 287-303
Author(s):  
Amalendu Ghosh ◽  
Heena Dhall ◽  
Ralf G. Dietzgen ◽  
Rakesh Kumar Jain
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Plant Virus ◽  
Insect Cell Culture ◽  
Historical Overview ◽  
Virus Research

scholarly journals Arabidopsis thaliana as a model for the study of plant–virus co-evolution

2010 ◽  
Vol 365 (1548) ◽  
pp. 1983-1995 ◽  
Author(s):  
Israel Pagán ◽  
Aurora Fraile ◽  
Elena Fernandez-Fueyo ◽  
Nuria Montes ◽  
Carlos Alonso-Blanco ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Plant Virus ◽  
Cauliflower Mosaic Virus ◽  
Growth Stages ◽  
Virus Species ◽  
Life Styles ◽  
Two Factors ◽  
Virus Genotypes

Understanding plant–virus coevolution requires wild systems in which there is no human manipulation of either host or virus. To develop such a system, we analysed virus infection in six wild populations of Arabidopsis thaliana in Central Spain. The incidence of five virus species with different life-styles was monitored during four years, and this was analysed in relation to the demography of the host populations. Total virus incidence reached 70 per cent, which suggests a role of virus infection in the population structure and dynamics of the host, under the assumption of a host fitness cost caused by the infection. Maximum incidence occurred at early growth stages, and co-infection with different viruses was frequent, two factors often resulting in increased virulence. Experimental infections under controlled conditions with two isolates of the most prevalent viruses, cauliflower mosaic virus and cucumber mosaic virus, showed that there is genetic variation for virus accumulation, although this depended on the interaction between host and virus genotypes. Comparison of Q ST -based genetic differentiations between both host populations with F ST genetic differentiation based on putatively neutral markers suggests different selection dynamics for resistance against different virus species or genotypes. Together, these results are compatible with a hypothesis of plant–virus coevolution.

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