The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and
PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were
evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive-
apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5
mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples
were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment),
11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular
vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups,
83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone
concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared
to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum
progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th
(4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level
at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became
pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/
ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/
ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0
(12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and
40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level
found at 40th day might be the influence of the non-pregnant does at first service in the cumulative
animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy
in treated and control animals.