Large-Scale Isolation of Cytochrome b 6/f Complex from Spinach Chloroplasts

1987 ◽  
pp. 173-176
Author(s):  
Michael T. Black ◽  
William R. Widger ◽  
William A. Cramer
Keyword(s):  
Cytochrome B ◽  
Large Scale ◽  
Spinach Chloroplasts

scholarly journals Cytochrom-b-563 als natürlicher Elektronenüberträger in einem zyklischen Elektronentransport der Photosynthese

1968 ◽  
Vol 23 (2) ◽  
pp. 235-238 ◽  
Author(s):  
J. Weikard
Keyword(s):  
Cytochrome B ◽  
Electron Acceptor ◽  
Red Light ◽  
Cyclic Flow ◽  
Spinach Chloroplasts ◽  
The Difference

By excitation of spinach chloroplasts with flashes (5 · 10-5 sec) of far red light hvI (λ=728 nm) and with addition of 2·10-4M/I benzylviologen as electron acceptor irreversible absorption changes are observed as depicted in fig. 1 top. With 10-3 M/l K3Fe (CN)6 as electron acceptor irreversible changes are observed as depicted in fig. 1 bottom. The difference of the spectra in fig. 1 is presented in fig. 2. Fig. 2 corresponds to the spectrum which is caused by reduction of a cytochrome-b-563. Thus excluding K3Fe (CN)6 as electron acceptor cytochrome-b-563 can be reduced by hvI (λ=728 nm). A re-oxydation occurs by red light hvII (λ = 663 nm), s. figs. 3, 4. These results demonstrate a natural cyclic flow of electrons including both light reactions Chl-ai and Chl-aII (s. fig. 6).The results are reviewed in 1. c.10.

The peroxidase activity of cytochrome b6f complex from spinach chloroplasts

2010 ◽  
Vol 48 (1) ◽  
pp. 3-8
Author(s):  
X. B. Chen ◽  
C. Hou ◽  
L. B. Li ◽  
T. Y. Kuang
Keyword(s):  
Cytochrome B ◽  
Peroxidase Activity ◽  
Spinach Chloroplasts
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