Use of Urease Inhibitors in the Analysis of Urea and/or Ammonium from Urea-treated Soils

2002 ◽  
pp. 343-345
Author(s):  
S. Kiss ◽  
M. Simihăian
Keyword(s):  
Urease Inhibitors

Reactivity of Barbituric, Thiobarbituric Acids and Their Related Analogues: Synthesis of Substituted and Heterocycles-based Pyrimidines

2020 ◽  
Vol 24 (14) ◽  
pp. 1610-1642 ◽  
Author(s):  
Ahmed El-Mekabaty ◽  
Hassan A. Etman ◽  
Ahmed Mosbah ◽  
Ahmed A. Fadda
Keyword(s):  
Xanthine Oxidase ◽  
Multicomponent Reactions ◽  
Biological Activities ◽  
Present Review ◽  
Urease Inhibitors ◽  
Effective Catalyst ◽  
Inhibitory Activities ◽  
Catalyst Reusability ◽  
High Yields ◽  
Nano Catalysts

Barbituric, thiobarbituric acids and their related analogs are reactive synthons for the synthesis of drugs and biologically, and pharmaceutically active pyrimidines. The present review aimed to summarize the recent advances in the synthesis of different alkylsubstituted, fused cycles, spiro-, and binary heterocycles incorporated pyrimidine skeleton based on barbituric derivatives. In this sequence, the eco-friendly techniques under catalytic conditions were used for the diverse types of multicomponent reactions under different conditions for the synthesis of various types of heterocycles. Nano-catalysts are efficient for the synthesis of these compounds in high yields and effective catalyst reusability. The compounds are potent antibacterial, cytotoxic, xanthine oxidase inhibitory activities, and attend as urease inhibitors. The projected mechanisms for the synthesis of pyranopyrimidines, benzochromenopyrimidines, chromeno-pyranopyrimidines, spiroxyindoles, oxospiro-tricyclic furopyrimidines, pyrimidine-based monoand bicyclic pyridines were discussed. The potent and diverse biological activities for instance, antioxidant, antibacterial, cytotoxic, and xanthine oxidase inhibitory activities, as well as urease inhibitors, are specified.

Discovery and Study of the Binding Epitopes of Novel Urease Inhibitors by STD-NMR Spectroscopy and Biochemical Analyses

2013 ◽  
Vol 10 (6) ◽  
pp. 515-521 ◽  
Author(s):  
Atia-tul- Wahab ◽  
Ajmal Khan ◽  
Bishnu P. Marasini ◽  
M. Arif Lodhi ◽  
Atta-ur- Rahman ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Urease Inhibitors ◽  
Std Nmr ◽  
Biochemical Analyses

Synthesis and Structure−Activity Relationship Studies of N-monosubstituted Aroylthioureas as Urease Inhibitors

2020 ◽  
Vol 16 ◽  
Author(s):  
Wei-Wei Ni ◽  
Hai-Lian Fang ◽  
Ya-Xi Ye ◽  
Wei-Yi Li ◽  
Li Liu ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Mammalian Cells ◽  
Intact Cell ◽  
Linear Regression Analysis ◽  
Positive Control ◽  
Acetohydroxamic Acid ◽  
Urease Inhibitors ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Low Cytotoxicity

Background: Thiourea is a classical urease inhibitor usually as a positive control, and many N,N`-disubstituted thioureas have been determined as urease inhibitors. However, due to steric hindrance, N,N`-disubstituted thiourea motif could not bind urease as thiourea. On the contrary, N-monosubstituted thioureas with a tiny thiourea motif could theoretically bind into the active pocket as thiourea. Objective: A series of N-monosubstituted aroylthioureas were designed and synthesized for evaluation as urease inhibitors. Methods: Urease inhibition was determined by the indophenol method and IC50 values were calculated using computerized linear regression analysis of quantal log dose-probit functions. The kinetic parameters were estimated viasurface plasmon resonance (SPR) and by nonlinear regression analysis based on the mixed type inhibition model derived from Michaelis-Menten kinetics. Results: Compounds b2, b11and b19 reversibly inhibited urease with a mixed mechanism, and showed excellent potency against both cell-free urease and urease in intact cell, with IC50 values being 90-to 450-fold and 5-to 50-fold lower than the positive control acetohydroxamic acid, respectively. The most potent compound b11 showed IC50 value of 0.060 ±0.004μM against cell-free urease, which bound to urea binding site with a very low KDvalue (0.420±0.003nM) and a very long residence time (6.7 min). Compound b11was also demonstrated having very low cytotoxicity to mammalian cells. Conclusion: These results revealed that N-monosubstituted aroylthioureas clearly bind the active site of urease as expected, and represent a new class of urease inhibitors for the development of potential therapeutics against infections caused by ure-ase-containing pathogens.

3D-QSAR CoMFA studies on bis-coumarine analogues as urease inhibitors: A strategic design in anti-urease agents

2008 ◽  
Vol 16 (6) ◽  
pp. 3456-3461 ◽  
Author(s):  
Zaheer-ul-Haq ◽  
M. Arif Lodhi ◽  
Sarfraz Ahmad Nawaz ◽  
Sajid Iqbal ◽  
Khalid Mohammed Khan ◽  
...  
Keyword(s):  
3D Qsar ◽  
Urease Inhibitors ◽  
Strategic Design

Utility of anthranilic acid and diethylacetylenedicarboxylate for the synthesis of nitrogenous organo/organometallic compounds as urease inhibitors

2019 ◽  
Vol 352 (7) ◽  
pp. 1800314 ◽  
Author(s):  
Heba S. A. El‐Zahabi ◽  
Hanan G. Abdulwahab ◽  
Mastoura M. Edrees ◽  
Amany M. Hegab
Keyword(s):  
Anthranilic Acid ◽  
Organometallic Compounds ◽  
Urease Inhibitors

scholarly journals Monte-Carlo method-based QSAR model to discover phytochemical urease inhibitors using SMILES and GRAPH descriptors

2021 ◽  
pp. 1-10
Author(s):  
Kumar Sambhav Chopdar ◽  
Ganesh Chandra Dash ◽  
Pranab Kishor Mohapatra ◽  
Binata Nayak ◽  
Mukesh Kumar Raval
Keyword(s):  
Monte Carlo ◽  
Monte Carlo Method ◽  
Qsar Model ◽  
Urease Inhibitors

Novel thiobarbiturates as potent urease inhibitors with potential antibacterial activity: Design, synthesis, radiolabeling and biodistribution study

2020 ◽  
Vol 28 (23) ◽  
pp. 115759
Author(s):  
Hanan Gaber Abdulwahab ◽  
Marwa F. Harras ◽  
Nagwan Galal El Menofy ◽  
Amany M. Hegab ◽  
Basma M. Essa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Biodistribution Study ◽  
Urease Inhibitors ◽  
Design Synthesis ◽  
Activity Design

scholarly journals Complexes of N- and O-Donor Ligands as Potential Urease Inhibitors

ACS Omega ◽  
2020 ◽  
Vol 5 (17) ◽  
pp. 10200-10206 ◽  
Author(s):  
Syed Raza Shah ◽  
Zarbad Shah ◽  
Mohammed Khiat ◽  
Ajmal Khan ◽  
Leila R. Hill ◽  
...  
Keyword(s):  
Donor Ligands ◽  
Urease Inhibitors

The determination of urea in soil extracts and related samples—a review

Soil Research ◽  
2004 ◽  
Vol 42 (7) ◽  
pp. 709 ◽  
Author(s):  
David F. Lambert ◽  
John E. Sherwood ◽  
Paul S. Francis
Keyword(s):  
Enzymatic Hydrolysis ◽  
Flow Injection Analysis ◽  
Acidic Solution ◽  
Clinical Applications ◽  
Urease Inhibitors ◽  
Viable Option ◽  
Soil Extracts ◽  
Diacetyl Monoxime ◽  
Hydrolysis Of

Although the dominant methods for the determination of urea in clinical applications incorporate selective enzymatic hydrolysis of urea, the determination of urea in soil extracts is complicated by the presence of urease inhibitors. The spectrophotometric determination of urea with an acidic solution diacetyl monoxime and semicarbazide is a viable option but traditional manual procedures are time-consuming. New variations on these procedures, based on microplates or flow-injection analysis methodologies, allow a far greater number of samples to be analysed with high precision and sensitivity.

Sign in / Sign up

Export Citation Format

Share Document