Meiotic chromosome pairing in Stethophyma grossum spermatocytes studied by a surface-spreading and silver-staining technique

Chromosoma ◽  
1980 ◽  
Vol 78 (2) ◽  
pp. 187-201 ◽  
Author(s):  
G. H. Jones ◽  
B. M. N. Wallace

Meiotic chromosome pairing is a process that is amenable to genetic and experimental analysis. The combined use of these two approaches allows for the process to be dissected into several finite periods of time in which the developmental stages of pairing can be precisely located. Evidence is now available, in particular in plants, that shows that the pairing of homologous chromosomes, as observed at metaphase I, is affected by events occurring as early as the last premeiotic mitosis; and that the maintenance of this early determined state is subsequently maintained by constituents (presumably proteins) that are sensitive to either colchicine, temperature or gene control. A critical assessment of this evidence in wheat and a comparison of the process of pairing in wheat with the course of meiotic pairing in other plants and animals is presented.


1987 ◽  
Vol 33 (4) ◽  
pp. 561-563 ◽  
Author(s):  
J M Sheat ◽  
M A Lorier

Abstract Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.


1986 ◽  
Vol 28 (2) ◽  
pp. 278-281 ◽  
Author(s):  
E. M. Nowick

Meiotic chromosome pairing was examined in F1 hybrid regenerants from Oryza sativa (AA) × O. latifolia (CCDD) and O. glumaepatula (AcuAcu) × O. latifolia (CCDD) crosses produced through embryo culture. The average number of chromosome pairs in the O. sativa × O. latifolia regenerants ranged from 13.79 to 14.79. Ten to 18 bivalents were observed per cell. The average number of bivalents in the O. glumaepatula × O. latifolia regenerants ranged from 12.44 to 13.87, with 10–17 bivalents per cell. Some desynapsis occurred but 10 to 12 true bivalents remained at late metaphase in most cells. The high number of bivalents observed in the hybrids from these divergent parents indicates that a genetic system for pairing control similar to that in Triticum may be present in the Oryza genus.Key words: Oryza, embryo culture, meiosis.


Heredity ◽  
1990 ◽  
Vol 65 (1) ◽  
pp. 11-20 ◽  
Author(s):  
J Loidl ◽  
F Ehrendorfer ◽  
D Schweizer

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