Cholesterol level in peripheral blood lymphocytes: Relationship with ischemic heart disease in patients with various forms of hyperlipidemia

1993 ◽  
Vol 116 (5) ◽  
pp. 1349-1352 ◽  
Author(s):  
L. A. Bolkhova ◽  
I. V. Fuki ◽  
E. Yu. Solov'eva ◽  
V. A. Koshechkin ◽  
V. S. Repin
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Cholesterol Level ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Ischemic Heart ◽  
Blood Lymphocytes

Abstract MP243: Detection Of Mesenchymal Stem Cells In Mobilized Autologous Peripheral Blood Transplantation From Patients With Ischemic Heart Disease

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Hadyanto Lim ◽  
Umar Zein ◽  
Ilham Hariaji
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Peripheral Blood ◽  
Autologous Transplantation ◽  
White Blood Cells ◽  
Significant Rise ◽  
Ischemic Heart ◽  
Post Transplantation

Background: Mesenchymal stem cells (MSCs) improve the cardiac function and remodeling in patients with ischemic heart disease. However, their presence in the circulating peripheral blood and post-transplantation has not been fully elucidated. We aimed to investigate the effects of intravenous transplantation of mobilized autologous peripheral blood on the number of MSCs in patients with ischemic heart disease. Methods: Granulocyte-colony stimulating factor (G-CSF, 5.0 μg/kg/day) was given subcutaneously once a day for five days to 7 patients (4 women and 3 men, aged 54-69 years) with ischemic heart disease. Leukapheresis procedure was started on the day 5 of G-CSF using the Spectra Optia cell separator. Circulating and intravenous transplantation of autologous MSCs after leukapheresis were analyzed by flow cytometry. MSCs were identified on the basis of dual positive cells (CD73 + /CD105 + or CD90 + /CD73 + or CD90 + /CD105 + ) and detected as MSCs if a cluster of at least 10 cells could be found. Results: MSCs in the circulating peripheral blood and after transplantation were detected in 2 (28%) and 6 (85%) patients, respectively. The frequency of intravenous peripheral blood MSCs increased significantly after transplantation (from 32.57 ± 22.76 x10 -4 % to 58.57 ± 28.49 x 10 -4 % , p<0.001). Moreover, there were significant rise in the total white blood cells count (from 10.25 ± 4.86 x 10 3 /μl to 35.81 ± 7.07 x 10 3 /μl, p<0.001) and the levels of CD34 + cells (from 1.17 ± 0.93 cells/μL to 138.30 ± 11.26 cells/μL, p<0.001) after the infusion. Conclusions: The results show that intravenous transplantation of mobilized autologous peripheral blood increases the number of MSCs in patients with ischemic heart disease. Leukapheresis product of peripheral blood MSCs could therefore be a potential source for autologous transplantation in ischemic heart disease.

scholarly journals Dynamics of endothelian function in different treatment strategies in stable ischemic heart disease

2019 ◽  
Vol 11 (1) ◽  
pp. 5-12
Author(s):  
Sergey A. Sayganov ◽  
Anastasiia M. Kuzmina-Krutetskaia
Keyword(s):  
Endothelial Cells ◽  
Heart Disease ◽  
Endothelial Dysfunction ◽  
Endothelial Function ◽  
Ischemic Heart Disease ◽  
Peripheral Blood ◽  
Ischemic Heart ◽  
Circulating Endothelial Cells ◽  
Lipid Lowering ◽  
Stable Ischemic Heart Disease

Aim. To assess the dynamics of endothelial function by determining circulating endothelial cells in peripheral blood in patients with stable ischemic heart disease within various treatment approaches. Material and methods. The study involved 98 patients with stable ischemic heart disease and 30 patients in the control group without coronary atherosclerosis. Endothelial function was assessed by determining the number of circulating endothelial cells in peripheral blood using flow cytofluorimetry with antibodies to cell surface markers: CD146+CD45– at the baseline. Depending on the treatment tactics, the study participants were divided into 3 groups: the medicamental therapy group, the group of coronary stenting, and the group of coronary bypass surgery. After 3 months from the baseline, dinamics assessment of endothelial dysfunction was performed. Results. Endothelial dysfunction was observed in all patients with obstructive lesions of the coronary blood flow and associated with effort angina class and anatomical severity of coronary disease. Improvement of endothelial function was facilitated by lipid-lowering therapy. Revascularization by coronary stenting impaired endothelial function in three months after the intervention. Mycoardial revascularization by coronary bypass did not impair endothelial function. Conclusion. Examination of endothelial dysfunction by determining the number of circulating endothelial cells in peripheral blood can be used to assess the severity of endothelial dysfunction in patients with IHD receiving lipid-lowering therapy and undergoing surgical revascularization.

ISOLATION AND CHARACTERISTICS OF COLONY-FORMING ENDOTHELIAL CELLS FROM PERIPHERAL BLOOD IN PATIENTS WITH ISCHEMIC HEART DISEASE

Tsitologiya ◽  
2018 ◽  
Vol 60 (8) ◽  
pp. 598-608
Author(s):  
V. G. Matveeva ◽  
◽  
M. Yu. Khanova ◽  
E. A. Velikanova ◽  
L. V. Antonova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Peripheral Blood ◽  
Ischemic Heart

scholarly journals Dynamics of the content of biologically active substances in peripheral blood neutrophils in patients with ischemic heart disease during the course of laser therapy

2012 ◽  
Vol 93 (1) ◽  
pp. 22-25
Author(s):  
M Yu Sapozhnikov
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Laser Therapy ◽  
Peripheral Blood ◽  
Biologically Active ◽  
Ischemic Heart ◽  
Healthy Individuals ◽  
Blood Neutrophils ◽  
Active Substances ◽  
Basic Treatment

Aim. To study the dynamics of the content of catecholamines, serotonin, histamine and heparin in peripheral blood neutrophils of patients with ischemic heart disease under the influence of laser therapy. Methods. Examined were 124 patients with angina, who were divided into two groups: those receiving basic treatment (β-blockers, antiplatelet drugs, nitrates, statins) with a course of laser therapy - 76 people, and those receiving only basic treatment - 48 people. The control group included 32 healthy individuals. Results. In patients with angina the content of catecholamines, serotonin, histamine is higher, while the content of heparin is lower than in healthy individuals. Under the influence of laser therapy, which was conducted simultaneously with the basic treatment, a decrease occurred in the level of catecholamines by 1.6 times, of serotonin by 1.5 times, of histamine by 1.6 times and an increase occurred in the amount of heparin by 1.9 times. Conclusion. Laser therapy effects the contents of all studied biologically active substances, bringing them closer to those of healthy people.

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