scholarly journals Dynamics of the content of biologically active substances in peripheral blood neutrophils in patients with ischemic heart disease during the course of laser therapy

2012 ◽  
Vol 93 (1) ◽  
pp. 22-25
Author(s):  
M Yu Sapozhnikov
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Laser Therapy ◽  
Peripheral Blood ◽  
Biologically Active ◽  
Ischemic Heart ◽  
Healthy Individuals ◽  
Blood Neutrophils ◽  
Active Substances ◽  
Basic Treatment

Aim. To study the dynamics of the content of catecholamines, serotonin, histamine and heparin in peripheral blood neutrophils of patients with ischemic heart disease under the influence of laser therapy. Methods. Examined were 124 patients with angina, who were divided into two groups: those receiving basic treatment (β-blockers, antiplatelet drugs, nitrates, statins) with a course of laser therapy - 76 people, and those receiving only basic treatment - 48 people. The control group included 32 healthy individuals. Results. In patients with angina the content of catecholamines, serotonin, histamine is higher, while the content of heparin is lower than in healthy individuals. Under the influence of laser therapy, which was conducted simultaneously with the basic treatment, a decrease occurred in the level of catecholamines by 1.6 times, of serotonin by 1.5 times, of histamine by 1.6 times and an increase occurred in the amount of heparin by 1.9 times. Conclusion. Laser therapy effects the contents of all studied biologically active substances, bringing them closer to those of healthy people.

Abstract MP243: Detection Of Mesenchymal Stem Cells In Mobilized Autologous Peripheral Blood Transplantation From Patients With Ischemic Heart Disease

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Hadyanto Lim ◽  
Umar Zein ◽  
Ilham Hariaji
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Peripheral Blood ◽  
Autologous Transplantation ◽  
White Blood Cells ◽  
Significant Rise ◽  
Ischemic Heart ◽  
Post Transplantation

Background: Mesenchymal stem cells (MSCs) improve the cardiac function and remodeling in patients with ischemic heart disease. However, their presence in the circulating peripheral blood and post-transplantation has not been fully elucidated. We aimed to investigate the effects of intravenous transplantation of mobilized autologous peripheral blood on the number of MSCs in patients with ischemic heart disease. Methods: Granulocyte-colony stimulating factor (G-CSF, 5.0 μg/kg/day) was given subcutaneously once a day for five days to 7 patients (4 women and 3 men, aged 54-69 years) with ischemic heart disease. Leukapheresis procedure was started on the day 5 of G-CSF using the Spectra Optia cell separator. Circulating and intravenous transplantation of autologous MSCs after leukapheresis were analyzed by flow cytometry. MSCs were identified on the basis of dual positive cells (CD73 + /CD105 + or CD90 + /CD73 + or CD90 + /CD105 + ) and detected as MSCs if a cluster of at least 10 cells could be found. Results: MSCs in the circulating peripheral blood and after transplantation were detected in 2 (28%) and 6 (85%) patients, respectively. The frequency of intravenous peripheral blood MSCs increased significantly after transplantation (from 32.57 ± 22.76 x10 -4 % to 58.57 ± 28.49 x 10 -4 % , p<0.001). Moreover, there were significant rise in the total white blood cells count (from 10.25 ± 4.86 x 10 3 /μl to 35.81 ± 7.07 x 10 3 /μl, p<0.001) and the levels of CD34 + cells (from 1.17 ± 0.93 cells/μL to 138.30 ± 11.26 cells/μL, p<0.001) after the infusion. Conclusions: The results show that intravenous transplantation of mobilized autologous peripheral blood increases the number of MSCs in patients with ischemic heart disease. Leukapheresis product of peripheral blood MSCs could therefore be a potential source for autologous transplantation in ischemic heart disease.

scholarly journals Dynamics of endothelian function in different treatment strategies in stable ischemic heart disease

2019 ◽  
Vol 11 (1) ◽  
pp. 5-12
Author(s):  
Sergey A. Sayganov ◽  
Anastasiia M. Kuzmina-Krutetskaia
Keyword(s):  
Endothelial Cells ◽  
Heart Disease ◽  
Endothelial Dysfunction ◽  
Endothelial Function ◽  
Ischemic Heart Disease ◽  
Peripheral Blood ◽  
Ischemic Heart ◽  
Circulating Endothelial Cells ◽  
Lipid Lowering ◽  
Stable Ischemic Heart Disease

Aim. To assess the dynamics of endothelial function by determining circulating endothelial cells in peripheral blood in patients with stable ischemic heart disease within various treatment approaches. Material and methods. The study involved 98 patients with stable ischemic heart disease and 30 patients in the control group without coronary atherosclerosis. Endothelial function was assessed by determining the number of circulating endothelial cells in peripheral blood using flow cytofluorimetry with antibodies to cell surface markers: CD146+CD45– at the baseline. Depending on the treatment tactics, the study participants were divided into 3 groups: the medicamental therapy group, the group of coronary stenting, and the group of coronary bypass surgery. After 3 months from the baseline, dinamics assessment of endothelial dysfunction was performed. Results. Endothelial dysfunction was observed in all patients with obstructive lesions of the coronary blood flow and associated with effort angina class and anatomical severity of coronary disease. Improvement of endothelial function was facilitated by lipid-lowering therapy. Revascularization by coronary stenting impaired endothelial function in three months after the intervention. Mycoardial revascularization by coronary bypass did not impair endothelial function. Conclusion. Examination of endothelial dysfunction by determining the number of circulating endothelial cells in peripheral blood can be used to assess the severity of endothelial dysfunction in patients with IHD receiving lipid-lowering therapy and undergoing surgical revascularization.

ISOLATION AND CHARACTERISTICS OF COLONY-FORMING ENDOTHELIAL CELLS FROM PERIPHERAL BLOOD IN PATIENTS WITH ISCHEMIC HEART DISEASE

Tsitologiya ◽  
2018 ◽  
Vol 60 (8) ◽  
pp. 598-608
Author(s):  
V. G. Matveeva ◽  
◽  
M. Yu. Khanova ◽  
E. A. Velikanova ◽  
L. V. Antonova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Peripheral Blood ◽  
Ischemic Heart

scholarly journals Statementtype in complex rehabilitation and secondary prevention of cardiovascular disorders in patients with coronary heart disease

2020 ◽  
Vol 97 (3) ◽  
pp. 57-64
Author(s):  
T.A. Knjazeva ◽  
V.A. Badtieva ◽  
T.I. Nikiforova
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Laser Therapy ◽  
Comparison Group ◽  
Infrared Laser ◽  
Main Group ◽  
Ischemic Heart ◽  
Complex Method ◽  
Cardiovascular Disorders ◽  
Surgical Revascularization

The single-center randomized comparative study on revealing the clinical efficacy of the complex method of rehabilitation and secondary prevention of cardiovascular disorders in patients with ischemic heart disease with the inclusion of physical training with the use of stabilometric complex with biofeedback (stabilo-training), general iodine-bromine baths and infrared laser therapy in patients with ischemic heart disease in the remote period after surgical revascularization of myocardium. Observation was made in 60 patients with ischemic heart disease, in remote, not earlier than one year, terms after surgical revascularization of myocardium. Patients were divided into 2 groups by simple randomization method. Group 1, the main group consisted of 30 patients who received a complex rehabilitation method with the inclusion of stabilizing training, general iodine-bromine baths and infrared laser therapy; group 2, the comparison included 30 patients who received a rehabilitation course with the inclusion of general iodine-bromine baths and infrared laser therapy only. Cardiovascular disorders (CVD) were evaluated using electrocardiography (ECG) in standard leads, echocardiography (ECG), daily ECG monitoring with assessment of cardiac rhythm variability, veloergometry (VEM), stabilometry and a 6-point rating scale and psychological testing with the help of HAM and Spielberg tests. The study results were processed using the Statistica 8 for Windows application package. Preliminary calculation of sample power showed a sufficient number of patients in groups of 30 people. Calculation of statistical reliability of the results was performed by the Student-Fisher criterion. The differences between the two mean values were considered reliable at p<0.05. Under the influence of the complex with inclusion of statokinesoterapy in patients with IHD of the main group in comparison with the comparison group, more expressed antianginal and antiarrhythmic effects, increase of tolerance of physical activity, physical efficiency were noted. Positive ECG dynamics, increased cardiac output fraction, improved mood and sleep were noted. Positive dynamics of SSN in 85% of patients of the main group and in 65% of the comparison group was accompanied by increased stability in the vertical posture, decreased walking precariousness, improved coordination and equilibrium, decreased indices of the pressure center deviation (CD) in frontal and sagittal directions. The average rate of displacement of CD and the total area of the statokinesiogram decreased only in the main group of patients, in the comparison group the stated indices of stabilometry remained without dynamics. The total dynamics index of symptom severity decreased by 11±0.03 points in the main group of patients and by 6.7±0.01 points in the comparison group. The revealed positive clinical effects in patients with IHD in the distant period after surgical revascularization of myocardium, a complex method with inclusion of stabilokinesotherapy, allows to recommend this method of sensomotor physical training in complex rehabilitation programs with the purpose of SSN correction, prevention of development of acute coronary syndrome and heart insufficiency.

scholarly journals Low-level laser therapy in rehabilitation of patients with ischemic heart disease: review of evidence-based studies

2019 ◽  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Laser Therapy ◽  
Rehabilitation Medicine ◽  
Ischemic Heart ◽  
Low Level Laser Therapy ◽  
Evidence Based ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Cardiac rehabilitation is one of the main achievements of cardiology and physical rehabilitation medicine in the second half of the 20th century. Based on the methodology of evidence-based medicine, cardiac rehabilitation in a short time evolved from a “fictional” “alternative” unproven treatment direction to completely uncontested and proven multidisciplinary programs. This article presents contemporary findings on cardiorehabilitation and analysis of evidence-based experimental and clinical studies of low-level laser therapy efficiency in patients with ischemic heart disease. Aim. To search for evidence-based investigations on application of low-level laser therapy for rehabilitation of patients with ischemic heart disease and to analyze their major propositions and recommendations.Materials and methods. PEDro, EMBASE, PubMed and Cochrane library electronic databases were searched for randomized clinical trials, systematic reviews, meta-analyses and clinical protocols using the key words within 2000–2019 years. Results and discussion: experimental and clinical investigations were carefully considered and the following findings were analyzed: action mechanisms of low-level laser therapy, its positive influence on myocardial remodeling, infarction area reduction, restenosis prevention and presented cardioprotective effect. More attention was focused at clinical evidence-based studies on application of photobiomodulation in order to increase the efficacy of surgical and percutaneous myocardial revascularization, and in non-invasive cardiology and physical rehabilitation medicine. Conclusion: regular analysis of performed studies and realization of new evidence-based investigation should be conducted to evaluate the efficiency of low-level laser therapy in rehabilitation of patients with ischemic heart disease.

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