The marine microalgaChlorella stigmatophora as a potential source of single cell protein: Enhancement of the protein content in response to nutrient enrichment

This study aimed to determine the growth rate and protein content in Nannochloropsis oculata, Chlorella vulgaris, Spirulina platensis were cultured using agricultural fertilizers. The agricultural fertilizers used were Urea, ZA and TSP. Each single cell protein was cultured using the three types of fertilizers with a ratio of Urea: ZA and TSP as follows (A) 1: 2: 1 [(10:20:10 g / L)], (B) 2: 2: 1 [(20:20:10 g / L)] and (C) 3: 2: 1 [(30:20:10 g / L)], respectively. The results showed that the best ratio of Urea, ZA and TSP fertilizers to growth of Spirulina, Nannochloropsis oculata and Chlorella vulgaris was C [30:20:10 (g / L)] treatment. However, phyto-protein content in Nannochlorophsis differed significantly between fertilization treatments, C fertilization yielded the highest protein content (28.75±0.05%), when compared with A (25.13±0.01%) and B (25.14±0.02%), respectively. In Chlorella vulgaris, all fertilization treatments showed very significant differences, B fertilization (28.24%±00.006) yielded the highest phyto-protein content, if compared to A (23.63% ± 0.003) and C (19.74% ±0.006 ), respectively. All fertilization treatments showed very significant differences (P <0.05) on Spirulina platensis. The highest content of phyto-protein (62.68 + 0.05%) was present in treatment C, when compared to A (52.18 + 0.05%) and B (62.37 + 0.01%) treatments, respectively.

Download Full-text

Growth of the Acid-Tolerant Fungus Scytalidium acidophilum as a Potential Source of Single-Cell Protein

Journal of Food Science ◽

10.1111/j.1365-2621.1985.tb13308.x ◽

2006 ◽

Vol 50 (1) ◽

pp. 197-200 ◽

Cited By ~ 13

Author(s):

A. M. MARTIN ◽

M. D. WHITE

Keyword(s):

Single Cell ◽

Single Cell Protein ◽

Cell Protein ◽

Potential Source ◽

Acid Tolerant

Download Full-text

Marine microalgae as a potential source of single cell protein (SCP)

Applied Microbiology and Biotechnology ◽

10.1007/bf00249940 ◽

1985 ◽

Vol 23 (2) ◽

pp. 110-113

Author(s):

Jaime Fabregas ◽

Concepcion Herrero

Keyword(s):

Single Cell ◽

Single Cell Protein ◽

Marine Microalgae ◽

Cell Protein ◽

Potential Source

Download Full-text

PRODUKSI PROTEIN SEL TUNGGAL DARI KULTUR Rhizopus oryzae DENGAN MEDIUM LIMBAH CAIR TAHU

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2016.v2.i2.5987 ◽

2016 ◽

Vol 2 (2) ◽

pp. 132-137 ◽

Cited By ~ 1

Author(s):

Leny Maryana ◽

Syariful Anam ◽

Arsa Wahyu Nugrahani

Keyword(s):

Single Cell ◽

Protein Content ◽

Skin Diseases ◽

Rhizopus Oryzae ◽

Single Cell Protein ◽

Cell Protein ◽

Total Protein Content ◽

Growth Substrate ◽

Fermentation Time ◽

Optimum Ph

Tofu wastewater is the origination of tofu’s production process that can degrade the quality of the environment if not handled properly. Another impact of this waste is the potential attack that could evoke the diseases such as diarrhea, skin diseases, and other illnesses for people who use the stream flow that has been polluted by this wastewater. However, the high-carbohydrate and protein of this waste may allow it to be used more as a microbial growth substrate to produce a Single-cell Protein. The purpose of this study is to determine the optimum incubation time and the highest protein content of Rhizopus oryzae in the medium of tofu wastewater based on the calculation of protein content percentage and the analysis of cell dry mass. And to know the optimum pH growth with the variation of pH growth which is about 4, 5, and 6. The calculation is performed in accordance with the fermentation time at 24, 48, and 72 hours. Total protein content calculated by the Kjeldahl method. The results showed the highest protein content obtained in the incubation period to 48 hours with an average percent of protein about 0.47%, 0.47%, 0.46% and a mass of cell with an average value of 0.77%, 0, 84% and 0.91%. The optimum pH of Rhizopus oryzae growth in the medium of tofu wastewater is about 5 pH. It can be concluded that the wastewater of tofu potentially used as a growth substrate of Rhizopus oryzae. Keywords: Wastewater of Tofu, Single-cell Protein, Rhizopus oryzae.

Download Full-text

Addition of Carbon Sources to Pineapple Waste Media in the Production of Single Cell Protein Biomass Saccharomyces cerevisiae

Jurnal Riset Biologi dan Aplikasinya ◽

10.26740/jrba.v2n2.p70-76 ◽

2020 ◽

Vol 2 (2) ◽

pp. 70

Author(s):

Anggi Nurmalasari ◽

Shinta Maharani

Keyword(s):

Single Cell ◽

Protein Content ◽

Carbon Sources ◽

Dry Weight ◽

Fermentation Medium ◽

Single Cell Protein ◽

Cell Protein ◽

Fermentation Time ◽

Pineapple Peel ◽

The Media

Single-cell protein (SCP) is the term used for crude or pure protein derived from simple single or multi-celled microorganisms. Pineapple peel contains monosaccharides as much as 10.8% so that it can be used as a fermentation medium in single-cell protein production. The purpose of this study was to determine the effect of adding carbon sources of fructose and sucrose on pH, cell dry weight, and protein content in the manufacture of single-cell proteins. This study used a completely randomized design (CRD) with two factors, namely the addition of carbon (fructose, sucrose, and control) and fermentation time (24.48, and 72 hours). The data analysis used the variance test and the Duncan Multiple Range Test (DMRT) continued to test with a confidence level of 95%. The results showed that the addition of carbon to the media had a very significant effect on media pH, cell dry weight, and protein content. In the medium with the addition of fructose it has a pH of 3.81; dry weight 0.4203 grams; and protein content 69.08/L. Whereas in the media with the addition of sucrose, the pH was 4.33, the dry weight of the cells was 0.3385 grams, and the cells had a protein content of 85.55 mg/L. The addition of a fructose carbon source gave the cell dry weight more than the addition of carbon sucrose

Download Full-text