The intraclonal and interclonal phenotypic heterogeneity in a rhabdomyosarcoma cell line with abortive imitation of embryonic myogenesis

1988 ◽  
Vol 55 (1) ◽  
Author(s):  
C. D. Gerharz ◽  
H. Gabbert ◽  
R. Moll ◽  
W. Meffln ◽  
R. Engers ◽  
...  
Keyword(s):  
Cell Line ◽  
Phenotypic Heterogeneity ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Embryonic Myogenesis

scholarly journals Tumor necrosis factor (TNF)-induced protein phosphorylation in a human rhabdomyosarcoma cell line is mediated by 60-kD TNF receptors (TR60)

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2211-2220 ◽  
Author(s):  
A Mire-Sluis ◽  
A Meager
Keyword(s):  
Tumor Necrosis Factor ◽  
Protein Phosphorylation ◽  
Cell Line ◽  
Tumor Necrosis ◽  
Tnf Alpha ◽  
Tnf Receptors ◽  
Protein Kinase C Inhibitors ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Necrosis Factor

Abstract In the present study, we used a cloned derivative, KYM-1D4, of the human rhabdomyosarcoma cell line, KYM-1, known to express high numbers of the two tumor necrosis factor (TNF) receptors, TR60 and TR80, and to be highly sensitive to TNF alpha-mediated cytotoxicity/antiproliferation, to investigate the role of TR60 and TR80 in protein phosphorylation. Using permeabilized KYM-1D4 cells, it was found that TNF alpha strongly induced phosphorylation of proteins of molecular weight 80, 65, 58, 42, and 30 kD. Addition of a monoclonal antibody (MoAb) against TR60 was shown to induce cytotoxicity/antiproliferation in KYM-1D4 cells and the same pattern of protein phosphorylation as TNF alpha, whereas addition of an MoAb against TR80 was both noncytotoxic and ineffective in inducing protein phosphorylation. In contrast, in a highly TNF alpha-resistant KYM-1- derived cell line, 37B8R, no protein phosphorylation was induced with either TNF alpha or the agonistic anti-TR60 MoAb. However, when 37B8R was allowed to revert to partial TNF sensitivity by culture in the absence of TNF alpha, the resultant cell line, 37B8S, was found to regain inducibility of protein phosphorylation by TNF alpha. These results indicate that expression of functional TR60 in KYM-1-related cell lines is principally involved in TNF-mediated cytotoxicity/antiproliferation and is necessary for the induction of protein phosphorylation. Nevertheless, the latter, although apparently strongly associated with cytotoxicity, was probably involved in protective mechanisms because protein kinase C inhibitors that inhibited TNF alpha and anti-TR60-induced phosphorylation increased the cytotoxic/antiproliferative response to these mediators.

scholarly journals Biosynthesis of type V procollagen by A204, a human rhabdomyosarcoma cell line.

1982 ◽  
Vol 257 (15) ◽  
pp. 9016-9024 ◽  
Author(s):  
K Alitalo ◽  
R Myllyla ◽  
H Sage ◽  
P Pritzl ◽  
A Vaheri ◽  
...  
Keyword(s):  
Cell Line ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Type V

Biosynthesis of 10 kDa and 7.5 kDa insulin-like growth factor II in a human rhabdomyosarcoma cell line

1993 ◽  
Vol 93 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Finn C. Nielsen ◽  
Gisela Haselbacher ◽  
Jan Christiansen ◽  
Mats Lake ◽  
Mette Grønborg ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Insulin Like Growth Factor ◽  
Rhabdomyosarcoma Cell Line ◽  
Factor Ii ◽  
Rhabdomyosarcoma Cell

scholarly journals Characterization of a New Human Embryonal Rhabdomyosarcoma Cell Line, RMS-GR

1998 ◽  
Vol 89 (5) ◽  
pp. 525-532 ◽  
Author(s):  
Juan Emilio Fernández ◽  
Jose Prados ◽  
Consolación Melguizo ◽  
Nicola Arena ◽  
Fabio Malavasi ◽  
...  
Keyword(s):  
Cell Line ◽  
Embryonal Rhabdomyosarcoma ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell

Low Concentrations of Actinomycin D Potentially Cause Therapeutic Differentiation in Human Rhabdomyosarcoma Cell Line RD

1996 ◽  
Vol 192 (2) ◽  
pp. 188-194 ◽  
Author(s):  
C. Melguizo ◽  
J. Prados ◽  
J.A. Marchal ◽  
A.E. Aránega ◽  
L. Alvarez ◽  
...  
Keyword(s):  
Cell Line ◽  
Actinomycin D ◽  
Rhabdomyosarcoma Cell Line ◽  
Low Concentrations ◽  
Rhabdomyosarcoma Cell
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