Biosynthesis of 10 kDa and 7.5 kDa insulin-like growth factor II in a human rhabdomyosarcoma cell line

1993 ◽  
Vol 93 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Finn C. Nielsen ◽  
Gisela Haselbacher ◽  
Jan Christiansen ◽  
Mats Lake ◽  
Mette Grønborg ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Insulin Like Growth Factor ◽  
Rhabdomyosarcoma Cell Line ◽  
Factor Ii ◽  
Rhabdomyosarcoma Cell

Retinoic acid regulates insulin-like growth factor II expression in a neuroblastoma cell line.

Endocrinology ◽  
1992 ◽  
Vol 130 (6) ◽  
pp. 3669-3676 ◽  
Author(s):  
K Matsumoto ◽  
C Gaetano ◽  
W H Daughaday ◽  
C J Thiele
Keyword(s):  
Retinoic Acid ◽  
Growth Factor ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Insulin Like Growth Factor ◽  
Factor Ii

scholarly journals Insulin-like growth factor-II gene expression in a rat insulin-producing beta-cell line (INS-1) is regulated by glucose

Diabetologia ◽  
1995 ◽  
Vol 38 (8) ◽  
pp. 927-935
Author(s):  
M. Asfari ◽  
W. De ◽  
M. N�el ◽  
P. E. Holthuizen ◽  
P. Czernichow
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Beta Cell ◽  
Cell Line ◽  
Insulin Like Growth Factor ◽  
Beta Cell Line ◽  
Factor Ii

The binding of insulin-like growth factor II to the erythroleukemia cell line K562

1984 ◽  
Vol 4 (12) ◽  
pp. 1071-1077 ◽  
Author(s):  
Michael Tally ◽  
Tang Xin-Zhi ◽  
Gösta Enberg ◽  
Kerstin Hall
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Specific Binding ◽  
Affinity Constant ◽  
Scatchard Analysis ◽  
Insulin Like Growth Factor ◽  
Factor Ii ◽  
Igf I ◽  
Erythroleukemia Cell ◽  
High Concentrations

The erythroleukemia cell line K562 was previously shown to have specific binding sites for insulin but not for insulin-like growth factor I (IGF-I). In this study the presence of specific receptors for insulin-like growth factor II (IGFqI) is established. Scatchard analysis of the competition curve for IGF-II disclosed a non-cooperative binding kinetic with a calculated affinity constant of 2.4 × 108 M−1 and a receptor number of 4.8 × 104 sites/cell. IGF-I displayed 10% crossreactivity over the IGF-II receptor but insulin did not crossreact at all. Instead insulin, present in high concentrations, enhanced the binding of IGF-II. The presence of IGF II but not IGF-I receptors makes the K562 cell line suitable for studying properties of the type-2 receptor.

Sign in / Sign up

Export Citation Format

Share Document