High carriage of plasmid-mediated quinolone resistance (PMQR) genes by cefotaxime-resistant Escherichia coli recovered from surface-leaking sanitary sewers

2022 ◽  
Vol 204 (2) ◽  
Author(s):  
Abimbola Olumide Adekanmbi ◽  
Olabisi Comfort Akinlabi ◽  
Adedolapo Victoria Olaposi
Keyword(s):  
Escherichia Coli ◽  
Quinolone Resistance ◽  
Sanitary Sewers

scholarly journals High Carriage of Plasmid-Mediated Quinolone Resistance (PMQR) Genes by Cefotaxime-Resistant Escherichia Coli Recovered from Surface-Leaking Sanitary Sewers

2021 ◽  
Author(s):  
Abimbola Olumide Adekanmbi ◽  
Olabisi C. Akinlabi ◽  
Adedolapo V. Olaposi
Keyword(s):  
Escherichia Coli ◽  
Brain Heart Infusion ◽  
Quinolone Resistance ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Specific Pcr ◽  
Sanitary Sewers ◽  
High Level

Abstract There is a rapid rise in the incident of quinolone resistant bacteria in Nigeria. Most studies in Nigeria have focused on isolates from the clinical settings, with few focusing on isolates of environmental origin. This study aimed to investigate the antibiogram and carriage of plasmid-mediated quinolone resistance (PMQR) genes by quinolone-resistant isolates obtained from a pool of cefotaxime-resistant Escherichia coli (E. coli) recovered from sewage leaking out of some broken sanitary sewers in a University community in Nigeria. Isolation of E. coli from the sewage samples was done on CHROMagar E. coli after enrichment of the samples was done in Brain Heart Infusion amended with 6µg/mL of cefotaxime. Identification of presumptive E. coli was done using molecular methods (detection of uidA gene), while susceptibility to antibiotics was carried out using disc-diffusion method. Detection of PMQR genes (qnrA, qnrB, qnrS, aac(6')-lb-cr, qepA and oqxAB) was done using primer-specific PCR. A total of 32 non-repetitive cefotaxime-resistant E. coli were obtained from the sewage, with 21 being quinolone-resistant. The quinolone-resistant isolates showed varying level of resistance to the tested antibiotics, with imipenem being the only exception with 0% resistance. The PMQR genes: aac(6')-lb-cr, qnrA, qnrB, qnrS and qepA and oqxAB were detected in 90.5%, 61.9%, 47.6%, 38.1%, 4.8% and 0% respectively of the isolates. The findings of this study showed a high level of resistance to antibiotics and carriage of PMQR genes by quinolone-resistant E. coli obtained from the leaking sanitary sewers, suggesting a potential environmental and public health concern.

scholarly journals Relationship between hospital antibiotic use and quinolone resistance in Escherichia coli

2013 ◽  
Vol 17 (4) ◽  
pp. e254-e258 ◽  
Author(s):  
Eric Batard ◽  
Florence Ollivier ◽  
David Boutoille ◽  
Jean-Benoît Hardouin ◽  
Emmanuel Montassier ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Use ◽  
Quinolone Resistance

scholarly journals Contributions of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals.

1996 ◽  
Vol 40 (10) ◽  
pp. 2380-2386 ◽  
Author(s):  
M J Everett ◽  
Y F Jin ◽  
V Ricci ◽  
L J Piddock
Keyword(s):  
Escherichia Coli ◽  
Dna Sequences ◽  
Quinolone Resistance ◽  
Fluoroquinolone Resistance ◽  
Polymorphism Analysis ◽  
Single Mutation ◽  
Wild Type ◽  
Single Strand Conformational Polymorphism ◽  
E Coli ◽  
High Level

Twenty-eight human isolates of Escherichia coli from Argentina and Spain and eight veterinary isolates received from the Ministry of Agriculture Fisheries and Foods in the United Kingdom required 2 to > 128 micrograms of ciprofloxacin per ml for inhibition. Fragments of gyrA and parC encompassing the quinolone resistance-determining region were amplified by PCR, and the DNA sequences of the fragments were determined. All isolates contained a mutation in gyrA of a serine at position 83 (Ser83) to an Leu, and 26 isolates also contained a mutation of Asp87 to one of four amino acids: Asn (n = 14), Tyr (n = 6), Gly (n = 5), or His (n = 1). Twenty-four isolates contained a single mutation in parC, either a Ser80 to Ile (n = 17) or Arg (n = 2) or a Glu84 to Lys (n = 3). The role of a mutation in gyrB was investigated by introducing wild-type gyrB (pBP548) into all isolates; for three transformants MICs of ciprofloxacin were reduced; however, sequencing of PCR-derived fragments containing the gyrB quinolone resistance-determining region revealed no changes. The analogous region of parE was analyzed in 34 of 36 isolates by single-strand conformational polymorphism analysis and sequencing; however, no amino acid substitutions were discovered. The outer membrane protein and lipopolysaccharide profiles of all isolates were compared with those of reference strains, and the concentration of ciprofloxacin accumulated (with or without 100 microM carbony cyanide m-chlorophenylhydrazone [CCCP] was determined. Twenty-two isolates accumulated significantly lower concentrations of ciprofloxacin than the wild-type E. coli isolate; nine isolates accumulated less then half the concentration. The addition of CCCP increased the concentration of ciprofloxacin accumulated, and in all but one isolate the percent increase was greater than that in the control strains. The data indicate that high-level fluoroquinolone resistance in E. coli involves the acquisition of mutations at multiple loci.

scholarly journals Prevalence of Plasmid-Mediated Quinolone Resistance Genes Among Escherichia coli in the Gut of Healthy People in Fuzhou, China

2018 ◽  
Vol 38 (4) ◽  
pp. 384-386 ◽  
Author(s):  
Bin Li ◽  
Yao Chen ◽  
Zhiyun Wu ◽  
Zhichang Zhao ◽  
Juan Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Healthy People ◽  
Quinolone Resistance

scholarly journals Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli

2015 ◽  
Vol 46 (3) ◽  
pp. 753-757
Author(s):  
Franciane Gomig ◽  
Carolina Weigert Galvão ◽  
Denis Leandro de Freitas ◽  
Larissa Labas ◽  
Rafael Mazer Etto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quinolone Resistance

scholarly journals Surveillance of antimicrobial-resistant Escherichia coli in Sheltered dogs in the Kanto Region of Japan

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Akihisa Hata ◽  
Noboru Fujitani ◽  
Fumiko Ono ◽  
Yasuhiro Yoshikawa
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Quinolone Resistance ◽  
Resistant Bacteria ◽  
Animal Shelters ◽  
E Coli ◽  
Chiba Prefecture ◽  
Shelter Dogs ◽  
Kanagawa Prefecture ◽  
Kanto Region

AbstractThere is a lack of an established antimicrobial resistance (AMR) surveillance system in animal welfare centers. Therefore, the AMR prevalence in shelter dogs is rarely known. Herein, we conducted a survey in animal shelters in Chiba and Kanagawa prefectures, in the Kanto Region, Japan, to ascertain the AMR status of Escherichia coli  (E. coli) prevalent in shelter dogs. E. coli was detected in the fecal samples of all 61 and 77 shelter dogs tested in Chiba and Kanagawa, respectively. The AMR was tested against 20 antibiotics. E. coli isolates derived from 16.4% and 26.0% of samples from Chiba and Kanagawa exhibited resistance to at least one antibiotic, respectively. E. coli in samples from Chiba and Kanagawa prefectures were commonly resistant to ampicillin, piperacillin, streptomycin, kanamycin, tetracycline, and nalidixic acid; that from the Kanagawa Prefecture to cefazolin, cefotaxime, aztreonam, ciprofloxacin, and levofloxacin and that from Chiba Prefecture to chloramphenicol and imipenem. Multidrug-resistant bacteria were detected in 18 dogs from both regions; β-lactamase genes (blaTEM, blaDHA-1, blaCTX-M-9 group CTX-M-14), quinolone-resistance protein genes (qnrB and qnrS), and mutations in quinolone-resistance-determining regions (gyrA and parC) were detected. These results could partially represent the AMR data in shelter dogs in the Kanto Region of Japan.

scholarly journals Quinolone resistance in Escherichia coli

2001 ◽  
Vol 32 (3/4) ◽  
pp. 275-284 ◽  
Author(s):  
Mark Webber ◽  
Laura J.V. Piddock
Keyword(s):  
Escherichia Coli ◽  
Quinolone Resistance
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