Clinical Potential of miR-451 and miR-506 as a Prognostic Biomarker in Patients with Breast Cancer

Background. The incidence and mortality of breast cancer in the world remain high. The function and important role of miR-451 and miR-506 in a series of cancers have been proved. The purpose of this research was to explore the clinical diagnosis and prognostic significance of miR-451 and miR-506 expression in breast cancer. Methods. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect miR-451 and miR-506 expression in serum and tissues. The relationship of miR-451 and miR-506 with clinical parameters was determined by the chi-square test. Receiver operating characteristics (ROC) analysis was conducted to evaluate the diagnostic accuracy of miR-451 and miR-506 in breast cancer. In addition, we determined the prognostic performance of miR-451 and miR-506 using Kaplan–Meier survival assay. Results. The expression of miR-451 and miR-506 in breast cancer patients was significantly lower than that in healthy people. miR-451 and miR-506 expression decreased in breast cancer tissues compared with paracancerous tissue. High expression of miR-451 and miR-506 was associated with positive lymph node metastasis and late tumor node metastasis stage. Breast cancer patients with high miR-451 and miR-506 expression had lower five-year survival rate. The level of miR-451 and miR-506 expression showed high diagnostic accuracy for distinguishing breast cancer patients and healthy people. Conclusion. miR-451 and miR-506 could be used as biomarker for the diagnosis and prognosis of breast cancer.

Health-Related Quality of Life (HRQoL) of People over 65 Years of Age

Quality of life is an important indicator of the treatment process, lifestyle, and influence of many other factors, both exogenous and endogenous, on the body. Determining the quality of life of healthy people (health-related quality of life (HRQoL), considering the influence of various factors, is important due to the possibility of making subsequent comparative analyses regarding the quality of life of people diagnosed with diseases. In addition, it allows us to identify the most crucial factors influencing the HRQoL in the process of “good aging”. The purpose of the study was to present the HRQoL level of healthy people over 65 years of age. HRQoL was measured in five-year age groups (66–70, 71–75, 76–80, >80 years), considering the analyzed factors. Finally, 1038 healthy people were included in the study. The inclusion criteria were as follows: no diagnosed chronic diseases, no permanent treatment in specialist clinics, and no constant administration of medicaments. A comparative analysis was carried out, assuming a 5% conclusion error. The SF-36 questionnaire assessing the main dimensions of the quality of life was the tool used in the study to assess the HRQoL: the physical component summary (PCS), mental component summary (MCS) and index of life quality (ILQ). The factors significantly differentiating the average level of HRQoL were as follows: gender, place of residence, education, employment status, smoking and physical activity. Relationship status (p > 0.05) was one of the analyzed factors that did not influence the differences in the average level of the perceived HRQoL. More than a twofold greater chance of a higher HRQoL was reported in the group of men under 75 years of age (66–70: OR = 2.01; 71–75: OR = 2.52) compared to the group of women. The same relationship was noted in the case of higher education in respondents up to the age of 80 (66–70: OR = 1.56; 71–75: OR = 2.16; 76–80: OR = 2.74). Smoking by people over 80 years of age significantly increased the chances of a higher HRQoL in each of the dimensions (PCS: OR = 4.09; MCS: OR = 12.64; ILQ: OR = 5.79). Age as a non-modifiable factor significantly differentiates the level of the HRQoL of healthy people over 65 years of age. The results of the conducted study on HRQoL can be helpful when comparing the HRQoL of healthy people with a group of people with chronic diseases.

Determining the Intensity of Basic Emotions among People Suffering from Anorexia Nervosa Based on Free Statements about Their Body

Objective: This study sought to address one of the challenges of psychiatry-computer aided diagnosis and therapy of anorexia nervosa. The goal of the paper is to present a method of determining the intensity of five emotions (happiness, sadness, anxiety, anger and disgust) in medical notes, which was then used to analyze the feelings of people suffering from anorexia nervosa. In total, 96 notes were researched (46 from people suffering from anorexia and 52 from healthy people). Method: The developed solution allows a comprehensive assessment of the intensity of five feelings (happiness, sadness, anxiety, anger and disgust) occurring in text notes. This method implements Nencki Affective Word List dictionary extension, in which the original version has a limited vocabulary. The method was tested on a group of patients suffering from anorexia nervosa and a control group (healthy people without an eating disorder). Of the analyzed medical, only 8% of the words are in the original dictionary. Results: As a result of the study, two emotional profiles were obtained: one pattern for a healthy person and one for a person suffering from anorexia nervosa. Comparing the average emotional intensity in profiles of a healthy person and person with a disorder, a higher value of happiness intensity is noticeable in the profile of a healthy person than in the profile of a person with an illness. The opposite situation occurs with other emotions (sadness, anxiety, disgust, anger); they reach higher values in the case of the profile of a person suffering from anorexia nervosa. Discussion: The presented method can be used when observing the patient’s progress during applied therapy. It allows us to state whether the chosen method has a positive effect on the mental state of the patient, and if his emotional profile is similar to the emotional profile of a healthy person. The method can also be used during first diagnosis visit.

Risk assessment of gastric cancer in the presence of Helicobacter pylori cagA and hopQII genes

Helicobacter pylori bacterium is one of the most common bacterial infections globally and is the leading cause of indigestion, gastric and duodenal ulcers, and gastric cancer. This bacterium can escape the antibacterial effects of stomach acid by adapting to the inner layers of the stomach. It combines with the natural sugars in the gastric mucosa. The compound is so effective that it makes bacterium resistant. For genes related to the pathogenesis of H. pylori, using the existence of genes such as cagA, hopQI, and hopQII, PCR is performed on some of these genes to amplify fragments of different lengths. One of the less-studied cases is that two or more pathogenic genes are simultaneously associated with H. pylori. This study examined the frequency of diseases and healthy individuals infected with H. pylori and cagA and hopQII genotypes. To diagnose H. pylori infection in healthy and stomach cancer patients, the PCR products are electrophoresed on the agarose gel after glmM gene amplification by PCR. To this aim, stomach tissue biopsies were used for patients, and saliva was used for healthy individuals. For this purpose, 150 gastric biopsy samples from stomach cancer patients and 150 saliva samples from healthy people were collected. Data showed a significant relationship between the coexistence of two genes, cagA and hopQII, and stomach cancer. 34.2% of patients and 10.1% of healthy individuals showed two genotypes, while other healthy people (89.9%) infected with H. pylori did not have this genotype. Therefore, the simultaneous presence of these two bacterial genes in human societies can be an essential biomarker for the diagnosis and prognosis of gastric cancer.

January 26 — International Day «Let's Talk»

January marks the beginning of the new year and the middle of winter, therefore, the holidays this month are celebrated accordingly, for example, in Canada and the United States, Walrus Day is celebrated on January 1 with the organization of traditional swims in ice water, but on January 5, it was proposed to celebrate Fasting Day, obviously, with the purpose of saving the body tired from the festive feasts. On January 14, in some countries, it is customary to celebrate an unusual day — the Day of Combating Procrastination, which in psychology means «constantly putting things off for later», some experts have already dubbed this fairly common feature «the disease of the 21st century». January 16 isWorld SnowDay and January 21 is International Hug Day. Besides, on the last Wednesday of January, concerned people proposed to establish Let's Talk Day, dedicated to the problem of difficult relationships between healthy people and patients with mental disorders. Canada was the initiator of the celebration of this day. Experts suggest that ordinary people protect themselves from mental illness by leading an active lifestyle, but does it always help? Let's try to figure it out in this article.

THE CONCEPTS OF IMAGINATION, FANTASIES, DREAMS, AND HALLUCINATIONS IN MENTALLY HEALTHY PEOPLE

Imagination, fantasies, dreams, and hallucinations are contiguous mental processes that reflect various forms of image processing at the internal level. The ability to maintain boundaries between them, reflecting as they do either external or profoundly internal, subjective reality (reality testing), is considered to be one of the most widely accepted criteria of mental health. Nevertheless, traditionally these processes have been investigated independently by different authors adopting different approaches, and there is a discernible lack of studies dedicated to the comparative analysis of these phenomena, both in their theoretical and empirical aspects. At the same time, such data could be used to develop diagnostic methods of investigating mental processes in normal conditions as well as in cases of mental disorders. The aim of this study is to investigate people's common ideas about imagination, fantasies, dreams and hallucinations, as well as the subjective experience of them in comparison with each other. The study's group of participants consisted of 45 nominally mentally healthy people (32 women and 13 men) aged between 17 and 29 years old. The following methods were used during the study: a semistructured interview aimed at studying the respondents' ideas about imagination, fantasy, dreams and hallucinations, and visual drawings of the forms, which these processes took. According to the results of the study, in the case of 15% of the respondents, their ideas about imagination, fantasies, dreams and hallucinations differed from their scientific definitions. The drawings of the images of imagination, fantasies, dreams and hallucinations varied in terms of emotional experience. The more the mental process is voluntary and subjectively controlled, the more these images are associated with positive emotions. In particular, images of imagination are mainly associated with a positive emotional charge, images of fantasy more often evoke positive emotions, but also ambivalent experiences, and drawings of the images of dreams and hallucinations are most often associated with negative emotions.

Study of the relationship between antibiotic resistance markers and virulence markers in NDM-positive Klebsiella pneumoniae strains circulating in various waters and human loci

Introduction. The propagation of multi-resistance to antibiotics among hospital isolates of Klebsiella pneumoniae (K. pneumoniae) is a subject of growing concern worldwide. At present, growing data of association between resistance and hypervirulence in clinical isolates of K. pneumoniae emerges. However, the occurrence of these pathogens in the environment remains an open question. The aim of this study was to evaluate and compare antibiotic resistance determinants occurrence in Klebsiella pneumoniae isolates from water sources (environmental and sewage), human sources (practically healthy people and patients with inflaammatory bowel disease (IBD), and extraintestinal infections (ExII)). Materials and methods. The PCR assay of carbapenemase genes IMP, NDM, VIM, KPC, OXA-48 was performed with the commercial “Amplisense” kits according to the manufacturer's instructions. The assay was used to evaluate the occurrence of antibiotic-resistance genes in 223 isolates of Klebsiella pneumoniae from various sources: 42 isolates from sewage, 19 isolates from surface water sources, 30 isolates from biological material (blood, urine, surgical wounds, bronchoalveolar lavage) of patients with extraintestinal infections (ExII), 69 isolates from patients with inflammatory bowel diseases (IBD), and 63 isolates from faeces of practically healthy people. Results. The ExII group revealed various antibiotic resistance genes. The most prevalent gene was OXA (30% had this gene only, other 26,6% had also KPC or NDM). NDM as the only resistance gene was observed in 23,3% of ExII isolates. KPC gene was observed in 3,3% of ExII group. Two isolates from IBD group contained NDM gene along with VIM gene. Only NDM gene was found in all the other groups of Klebsiella pneumoniae isolates (13-28% isolates in every group, no statistical difference). NDM was shown to be associated with virulence genes iutA and rmpA that are responsible for iron consumption and hypermucoid phenotype. Conclusion. The most abundant resistance genes in the studied Klebsiella pneumoniae isolates were NDM (13.5%) and OXA (8%). At the same time, NDM was the only gene found in all groups (11-28%). NDM metallobeta-lactamase gene was associated with rmpA and iutA genes, giving an example of the connection between virulence and resistance properties. A significant amount of resistant isolates from healthy donors and surface waters indicates the need for additional study of the role of NDM positive isolates in pathogenicity of Klebsiella pneumoniae.

Application of the constitutional-typological approach in the study of the morphometric features of the dentition in people with the brachypalatinal type of the palatine arch

The fundamental problem of precision medicine is the division of the population of sick and healthy people into separate individuals, differing in the likelihood of disease occurrence and the ability to respond to various types of therapy. This is mainly due to the individual, constitutional features of the structure, including the dentoalveolar system. The introduction of a constitutional-typological approach in a comprehensive study of the human dentition is an important task of modern dentistry, since it allows one to determine the characteristic morphological and functional characteristics of each individual individual to improve the methods of diagnosis and treatment of dentoalveolar pathology. The article examines the morphometric features of the dentoalveolar system in people with brachypalatinal («wide» and «low») type of palatine vault, as well as their relationship with the shape of dental arches and the size of the inter-incisal angle at a physiological occlusal norm. As a result of the study, the following regularity was determined: the brachypalatinal type of the palatine vault corresponds to «wide» dental arches of the brachygnathic type with an inter-incisal angle of more than 145 degrees. The value of the brachypalatinal index of the palatine fornix, as the ratio of the height of the palate to its width, exceeds 45 percent, and the gnathic index of the dental arches is 0.59 ± 0.03. The data obtained can be used in the clinic of orthodontics, orthopedic dentistry, maxillofacial surgery to assess the parameters of the hard palate, diagnose pathological forms of the palatine fornix and determine the effectiveness of the treatment.

The Relationship Between Gut Microbiome Features and Chemotherapy Response in Gastrointestinal Cancer

ObjectiveThe prognosis of advanced gastrointestinal cancer is poor. There are studies indicating that gut microbes might have the predictive ability to evaluate the outcome of cancer therapy, especially immunotherapy. There is limited evidence to date on the influence of microbes on chemotherapeutic response.DesignIn total, 130 patients with advanced or metastatic esophageal (n=40), gastric (n=46), and colorectal cancer (n=44) were enrolled. We included 147 healthy people as controls and used 16S rRNA sequencing to analyze the fecal microbiota.ResultsSignificant differences in the abundance of fecal microbiota between patients with gastrointestinal cancer and controls were identified. The abundance of Bacteroides fragilis, Escherichia coli, Akkermansia muciniphila, Clostridium hathewayi, and Alistipes finegoldii were significantly increased in the patient group. Faecalibacterium prausnitzii, Roseburia faecis, Clostridium clostridioforme, Blautia producta, Bifidobacterium adolescent, and Butyricicoccus pullicaecorum taxa were significantly more abundant in the controls. The amount of R. faecis in non-responders (NR) was more likely to decrease significantly after chemotherapy, while the amount mostly increased in responders (R) (P=0.040). The optimal abundance variation of R. faecis may be a predictor for distinguishing patients with PD from those with non-PD in all patients with gastrointestinal cancer, with a sensitivity of 75.0% and a specificity of 93.9%.ConclusionThe gut microbiome of patients with esophageal cancer, gastric cancer, and colorectal cancer differs from those of healthy people. The abundance alteration of R. faecis in patients with GI cancer might be a predictor of chemotherapy efficacy.

Assessment of Human Mycotoxin Exposure in Hungary by Urinary Biomarker Determination and the Uncertainties of the Exposure Calculation: A Case Study

Urinary biomarkers of mycotoxin exposure were evaluated in the case of healthy people (n = 41) and coeliac patients (n = 19) by using a multi-biomarker LC-MS/MS immunoaffinity based method capable to analyse biomarkers of nine mycotoxins, i.e., fumonisin B1 (FB1), fumonisin B2 (FB2), deoxynivalenol (DON), zearalenone (ZEN), ochratoxin A (OTA), Aflatoxin B1 (AFB1), T-2 toxin, HT-2 toxin and Nivalenol (NIV). Urinary biomarker concentrations were used to calculate the probable daily intake (PDI) of fumonisin B1, deoxynivalenol, zearalenone and ochratoxin A and compared with their tolerable daily intake (TDI). The human urinary excretion rate values reported in the literature and the 24 h excretion rate measured in piglets were used to estimate and compare the PDI values of the four mycotoxins. The highest mean biomarker concentrations were found for DON (2.30 ng/mL for healthy people and 2.68 ng/mL for coeliac patients). Mean OTA concentration was significantly higher (p < 0.001) in healthy people compared to coeliac patients. PDI calculated with piglets excretion data exceeded the TDI values by a much smaller percentage than when they were calculated from human data, especially for FB1. The uncertainties arising from the different calculations can be well perceived on the basis of these data.