Brucella melitensis invA gene (BME_RS01060) transcription is promoted under acidic stress conditions

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Raúl Sauceda-Becerra ◽  
Hugo Barrios-García ◽  
Julio Martínez-Burnes ◽  
Beatriz Arellano-Reynoso ◽  
Alejandro Benítez-Guzmán ◽  
...  
Keyword(s):  
Brucella Melitensis ◽  
Stress Conditions ◽  
Acidic Stress ◽  
Inva Gene

scholarly journals Biofilm Formation among Clinical and Food Isolates ofListeria monocytogenes

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Joana Barbosa ◽  
Sandra Borges ◽  
Ruth Camilo ◽  
Rui Magalhães ◽  
Vânia Ferreira ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Listeria Monocytogenes ◽  
Osmotic Stress ◽  
Environmental Conditions ◽  
Biofilm Formation ◽  
Stress Conditions ◽  
Acidic Stress ◽  
Biofilm Production ◽  
Oflisteria Monocytogenes ◽  
Clinical Cases

Objective. A total of 725Listeria monocytogenesisolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h.Methods. Biofilm production was carried out on polystyrene tissue culture plates. FiveL. monocytogenesisolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions.Results. Significant differences (P<0.01) between clinical and food isolates were observed. At 37°C for 24 h, most food isolates were classified as weak or moderate biofilm formers whereas all the clinical isolates were biofilm producers, although the majority were weak. At 4°C during 5 days, 65 and 59% isolates, from food and clinical cases, respectively, were classified as weak. After both sublethal stresses, at 37°C just one of the five isolates tested was shown to be more sensitive to subsequent acidic exposure. However, at 4°C both stresses did not confer either sensitivity or resistance.Conclusions. Significant differences between isolates origin, temperature, and sublethal acidic stress were observed concerning the ability to form biofilms. Strain, origin, and environmental conditions can determine the level of biofilm production byL. monocytogenesisolates.

Identification of OtpR regulated sRNAs in Brucella melitensis expressed under acidic stress and their roles in pathogenesis and metabolism

2017 ◽  
Vol 50 ◽  
pp. 40-47 ◽  
Author(s):  
Udayakumar S. Vishnu ◽  
Jagadesan Sankarasubramanian ◽  
Paramasamy Gunasekaran ◽  
Jeyaprakash Rajendhran
Keyword(s):  
Brucella Melitensis ◽  
Acidic Stress

scholarly journals Characterization of Heat, Oxidative, and Acid Stress Responses in Brucella melitensis

2000 ◽  
Vol 68 (5) ◽  
pp. 2954-2961 ◽  
Author(s):  
Ana P. Teixeira-Gomes ◽  
Axel Cloeckaert ◽  
Michel S. Zygmunt
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Stress Responses ◽  
Gel Electrophoresis ◽  
Brucella Melitensis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Acid Stress ◽  
Stress Conditions ◽  
Phagocytic Cells ◽  
Specific Regulation

ABSTRACT Brucella melitensis is a facultative intracellular pathogen which is able to survive and replicate within phagocytic cells. Therefore, it has to adapt to a range of different hostile environments. In order to understand the mechanisms of intracellular survival employed by virulent B. melitensis 16M, an initial approach consisting of analysis of the differences in patterns of protein synthesis in response to heat, oxidative, and acid pH stresses by two-dimensional (2-D) polyacrylamide gel electrophoresis was used. Depending on the stress, this involved about 6.4 to 12% of the 676 protein spots detected in 2-D gel electrophoresis. On the basis of N-terminal sequence analysis and database searching, 19 proteins whose level of synthesis was up- or down-regulated by stress conditions were identified. Some of them were previously reported forBrucella, such as BvrR, DnaK, GroEL, and Cu-Zn superoxide dismutase (SOD). Eight other proteins closely matched proteins found in other bacteria: AapJ, alpha-ETF, ClpP, Fe and/or Mn SOD, malate dehydrogenase, IalB, 30S ribosomal protein S1, and pyruvate dehydrogenase E1 component beta subunit. Results indicated thatB. melitensis could bring specific regulatory mechanisms into play in response to stress conditions. For example, the ribosome releasing factor in B. melitensis appeared to be a heat shock protein, whereas the ClpP protein, described as a heat shock protein for Escherichia coli, was strongly down-regulated in B. melitensis in response to heat stress. Some of the identified proteins and their potential specific regulation could be required for the adaptation of B. melitensis to environmental stresses encountered in phagocytic cells and possibly for bacterial virulence.

scholarly journals RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Wenxiao Liu ◽  
Hao Dong ◽  
Jing Li ◽  
Qixing Ou ◽  
Yujin Lv ◽  
...  
Keyword(s):  
Brucella Melitensis ◽  
Critical Role ◽  
Acidic Stress ◽  
Rna Seq

scholarly journals The Acidic Stress Response of the Intracellular Pathogen Brucella melitensis: New Insights from a Comparative, Genome-Wide Transcriptome Analysis

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1016
Author(s):  
David Kornspan ◽  
Tamar Zahavi ◽  
Mali Salmon-Divon
Keyword(s):  
Pathogenic Bacteria ◽  
Molecular Mechanisms ◽  
Brucella Melitensis ◽  
Virulent Strain ◽  
Acid Resistance ◽  
Differentially Expressed ◽  
In Vivo Model ◽  
Acidic Stress ◽  
Acidic Conditions

The intracellular pathogenic bacteria belonging to the genus Brucella must cope with acidic stress as they penetrate the host via the gastrointestinal route, and again during the initial stages of intracellular infection. A transcription-level regulation has been proposed to explain this but the specific molecular mechanisms are yet to be determined. We recently reported a comparative transcriptomic analysis of the attenuated vaccine Brucella melitensis strain Rev.1 against the virulent strain 16M in cultures grown under either neutral or acidic conditions. Here, we re-analyze the RNA-seq data of 16M from our previous study and compare it to published transcriptomic data of this strain from both an in cellulo and an in vivo model. We identify 588 genes that are exclusively differentially expressed in 16M grown under acidic versus neutral pH conditions, including 286 upregulated genes and 302 downregulated genes that are not differentially expressed in either the in cellulo or the in vivo model. Of these, we highlight 13 key genes that are known to be associated with a bacterial response to acidic stress and, in our study, were highly upregulated under acidic conditions. These genes provide new molecular insights into the mechanisms underlying the acid-resistance of Brucella within its host.

scholarly journals Brucella Melitensis invA Gene (BME_RS01060) Transcription Is Promoted Under Acidic Stress Conditions.

2021 ◽  
Author(s):  
Raúl Sauceda-Becerra ◽  
Hugo Barrios-García ◽  
Julio Martínez-Burnes ◽  
Beatriz Arellano-Reynoso ◽  
Alejandro Benítez-Guzmán ◽  
...  
Keyword(s):  
Real Time ◽  
Gene Transcription ◽  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Survival Rates ◽  
Time Dependent ◽  
Acidic Stress ◽  
Wild Type ◽  
Mutant Strains ◽  
Acidic Conditions

Abstract The invA gene of Brucella melitensis codes for a NUDIX (nucleoside diphosphate linked to moiety X) hydrolase related to invasiveness. The objective of this work was to evaluate invA transcription under acidic conditions. The invA gene transcription was up regulated at pH 3 and pH 5 observed with semiquantitative real-time PCR in B. melitensis 133 strain. Results indicated that invA gene transcription at pH 3 showed a basal and decreased transcription compared to that of pH 5 incubation. Transcription levels of the dnaK gene were similar to those obtained with invA gene. The survival rates of wild type and invA mutant strains at pH 5 were above 90% in all post-incubation times. In contrast, at pH 3 there was a time-dependent reduction on both strains at 15 min (P < 0.05). These results suggest that invA gene transcription is promoted under acidic conditions in Brucella melitensis.

Self-Injurious Behavior vs. Nonsuicidal Self-Injury

Crisis ◽  
2012 ◽  
Vol 33 (2) ◽  
pp. 106-112 ◽  
Author(s):  
Christopher M. Bloom ◽  
Shareen Holly ◽  
Adam M. P. Miller
Keyword(s):  
Positive Reinforcement ◽  
Empirical Investigation ◽  
High Stress ◽  
Animal Research ◽  
Stress Conditions ◽  
Human Populations ◽  
Pharmacological Interventions ◽  
Self Injury ◽  
Cns Stimulant ◽  
Nonsuicidal Self Injury

Background: Historically, the field of self-injury has distinguished between the behaviors exhibited among individuals with a developmental disability (self-injurious behaviors; SIB) and those present within a normative population (nonsuicidal self-injury; NSSI),which typically result as a response to perceived stress. More recently, however, conclusions about NSSI have been drawn from lines of animal research aimed at examining the neurobiological mechanisms of SIB. Despite some functional similarity between SIB and NSSI, no empirical investigation has provided precedent for the application of SIB-targeted animal research as justification for pharmacological interventions in populations demonstrating NSSI. Aims: The present study examined this question directly, by simulating an animal model of SIB in rodents injected with pemoline and systematically manipulating stress conditions in order to monitor rates of self-injury. Methods: Sham controls and experimental animals injected with pemoline (200 mg/kg) were assigned to either a low stress (discriminated positive reinforcement) or high stress (discriminated avoidance) group and compared on the dependent measures of self-inflicted injury prevalence and severity. Results: The manipulation of stress conditions did not impact the rate of self-injury demonstrated by the rats. The results do not support a model of stress-induced SIB in rodents. Conclusions: Current findings provide evidence for caution in the development of pharmacotherapies of NSSI in human populations based on CNS stimulant models. Theoretical implications are discussed with respect to antecedent factors such as preinjury arousal level and environmental stress.

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants
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