Identification of OtpR regulated sRNAs in Brucella melitensis expressed under acidic stress and their roles in pathogenesis and metabolism

2017 ◽  
Vol 50 ◽  
pp. 40-47 ◽  
Author(s):  
Udayakumar S. Vishnu ◽  
Jagadesan Sankarasubramanian ◽  
Paramasamy Gunasekaran ◽  
Jeyaprakash Rajendhran
Keyword(s):  
Brucella Melitensis ◽  
Acidic Stress

scholarly journals RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Wenxiao Liu ◽  
Hao Dong ◽  
Jing Li ◽  
Qixing Ou ◽  
Yujin Lv ◽  
...  
Keyword(s):  
Brucella Melitensis ◽  
Critical Role ◽  
Acidic Stress ◽  
Rna Seq

scholarly journals The Acidic Stress Response of the Intracellular Pathogen Brucella melitensis: New Insights from a Comparative, Genome-Wide Transcriptome Analysis

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1016
Author(s):  
David Kornspan ◽  
Tamar Zahavi ◽  
Mali Salmon-Divon
Keyword(s):  
Pathogenic Bacteria ◽  
Molecular Mechanisms ◽  
Brucella Melitensis ◽  
Virulent Strain ◽  
Acid Resistance ◽  
Differentially Expressed ◽  
In Vivo Model ◽  
Acidic Stress ◽  
Acidic Conditions

The intracellular pathogenic bacteria belonging to the genus Brucella must cope with acidic stress as they penetrate the host via the gastrointestinal route, and again during the initial stages of intracellular infection. A transcription-level regulation has been proposed to explain this but the specific molecular mechanisms are yet to be determined. We recently reported a comparative transcriptomic analysis of the attenuated vaccine Brucella melitensis strain Rev.1 against the virulent strain 16M in cultures grown under either neutral or acidic conditions. Here, we re-analyze the RNA-seq data of 16M from our previous study and compare it to published transcriptomic data of this strain from both an in cellulo and an in vivo model. We identify 588 genes that are exclusively differentially expressed in 16M grown under acidic versus neutral pH conditions, including 286 upregulated genes and 302 downregulated genes that are not differentially expressed in either the in cellulo or the in vivo model. Of these, we highlight 13 key genes that are known to be associated with a bacterial response to acidic stress and, in our study, were highly upregulated under acidic conditions. These genes provide new molecular insights into the mechanisms underlying the acid-resistance of Brucella within its host.

Brucella melitensis invA gene (BME_RS01060) transcription is promoted under acidic stress conditions

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Raúl Sauceda-Becerra ◽  
Hugo Barrios-García ◽  
Julio Martínez-Burnes ◽  
Beatriz Arellano-Reynoso ◽  
Alejandro Benítez-Guzmán ◽  
...  
Keyword(s):  
Brucella Melitensis ◽  
Stress Conditions ◽  
Acidic Stress ◽  
Inva Gene

scholarly journals Brucella Melitensis invA Gene (BME_RS01060) Transcription Is Promoted Under Acidic Stress Conditions.

2021 ◽  
Author(s):  
Raúl Sauceda-Becerra ◽  
Hugo Barrios-García ◽  
Julio Martínez-Burnes ◽  
Beatriz Arellano-Reynoso ◽  
Alejandro Benítez-Guzmán ◽  
...  
Keyword(s):  
Real Time ◽  
Gene Transcription ◽  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Survival Rates ◽  
Time Dependent ◽  
Acidic Stress ◽  
Wild Type ◽  
Mutant Strains ◽  
Acidic Conditions

Abstract The invA gene of Brucella melitensis codes for a NUDIX (nucleoside diphosphate linked to moiety X) hydrolase related to invasiveness. The objective of this work was to evaluate invA transcription under acidic conditions. The invA gene transcription was up regulated at pH 3 and pH 5 observed with semiquantitative real-time PCR in B. melitensis 133 strain. Results indicated that invA gene transcription at pH 3 showed a basal and decreased transcription compared to that of pH 5 incubation. Transcription levels of the dnaK gene were similar to those obtained with invA gene. The survival rates of wild type and invA mutant strains at pH 5 were above 90% in all post-incubation times. In contrast, at pH 3 there was a time-dependent reduction on both strains at 15 min (P < 0.05). These results suggest that invA gene transcription is promoted under acidic conditions in Brucella melitensis.

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