A method based on the ligation detection reaction–universal array (LDR–UA) for the detection and characterization of Listeria and Campylobacter strains

2010 ◽  
Vol 231 (6) ◽  
pp. 985-998 ◽  
Author(s):  
Andrea Lauri ◽  
Bianca Castiglioni ◽  
Marco Severgnini ◽  
Chiara Gorni ◽  
Paola Mariani
Keyword(s):  
Ligation Detection Reaction ◽  
Universal Array

A tool based on Ligation Detection Reaction-Universal Array (LDR-UA) for the characterization of VTEC by identification of virulence-associated and serogroup-specific genes

2011 ◽  
Vol 25 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Andrea Lauri ◽  
Bianca Castiglioni ◽  
Stefano Morabito ◽  
Rosangela Tozzoli ◽  
Clarissa Consolandi ◽  
...  
Keyword(s):  
Ligation Detection Reaction ◽  
Universal Array

Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach

2012 ◽  
Vol 153 (3) ◽  
pp. 474-482 ◽  
Author(s):  
Alessia Cariani ◽  
Annamaria Piano ◽  
Clarissa Consolandi ◽  
Marco Severgnini ◽  
Bianca Castiglioni ◽  
...  
Keyword(s):  
Ligation Detection Reaction ◽  
Universal Array ◽  
Pathogenic Vibrios

scholarly journals Development of a Universal Microarray Based on the Ligation Detection Reaction and 16S rRNA Gene Polymorphism To Target Diversity of Cyanobacteria

2004 ◽  
Vol 70 (12) ◽  
pp. 7161-7172 ◽  
Author(s):  
Bianca Castiglioni ◽  
Ermanno Rizzi ◽  
Andrea Frosini ◽  
Kaarina Sivonen ◽  
Pirjo Rajaniemi ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Environmental Samples ◽  
Rrna Gene ◽  
Accurate Identification ◽  
Culture Collections ◽  
Reliable Identification ◽  
Ligation Detection Reaction ◽  
Universal Array ◽  
Axenic Strains

ABSTRACT The cyanobacteria are photosynthetic prokaryotes of significant ecological and biotechnological interest, since they strongly contribute to primary production and are a rich source of bioactive compounds. In eutrophic fresh and brackish waters, their mass occurrences (water blooms) are often toxic and constitute a high potential risk for human health. Therefore, rapid and reliable identification of cyanobacterial species in complex environmental samples is important. Here we describe the development and validation of a microarray for the identification of cyanobacteria in aquatic environments. Our approach is based on the use of a ligation detection reaction coupled to a universal array. Probes were designed for detecting 19 cyanobacterial groups including Anabaena/Aphanizomenon, Calothrix, Cylindrospermopsis, Cylindrospermum, Gloeothece, halotolerants, Leptolyngbya, Palau Lyngbya, Microcystis, Nodularia, Nostoc, Planktothrix, Antarctic Phormidium, Prochlorococcus, Spirulina, Synechococcus, Synechocystis, Trichodesmium, and Woronichinia. These groups were identified based on an alignment of over 300 cyanobacterial 16S rRNA sequences. For validation of the microarrays, 95 samples (24 axenic strains from culture collections, 27 isolated strains, and 44 cloned fragments recovered from environmental samples) were tested. The results demonstrated a high discriminative power and sensitivity to 1 fmol of the PCR-amplified 16S rRNA gene. Accurate identification of target strains was also achieved with unbalanced mixes of PCR amplicons from different cyanobacteria and an environmental sample. Our universal array method shows great potential for rapid and reliable identification of cyanobacteria. It can be easily adapted to future development and could thus be applied both in research and environmental monitoring.

Olive variety identification by ligation detection reaction in a universal array format

2007 ◽  
Vol 129 (3) ◽  
pp. 565-574 ◽  
Author(s):  
Clarissa Consolandi ◽  
Luisa Palmieri ◽  
Silvia Doveri ◽  
Elena Maestri ◽  
Nelson Marmiroli ◽  
...  
Keyword(s):  
Variety Identification ◽  
Olive Variety ◽  
Ligation Detection Reaction ◽  
Universal Array ◽  
Array Format

scholarly journals Apolipoprotein E and Transferrin Genotyping by Ligation Detection Reaction and Universal Array

2003 ◽  
Vol 49 (9) ◽  
pp. 1537-1540 ◽  
Author(s):  
Roberta Bordoni ◽  
Bianca Castiglioni ◽  
Alessandra Mezzelani ◽  
Ermanno Rizzi ◽  
Andrea Frosini ◽  
...  
Keyword(s):  
Apolipoprotein E ◽  
Ligation Detection Reaction ◽  
Universal Array

scholarly journals Pathogen detection in milk samples by ligation detection reaction-mediated universal array method

2009 ◽  
Vol 92 (7) ◽  
pp. 3027-3039 ◽  
Author(s):  
P. Cremonesi ◽  
G. Pisoni ◽  
M. Severgnini ◽  
C. Consolandi ◽  
P. Moroni ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Milk Samples ◽  
Ligation Detection Reaction ◽  
Universal Array

scholarly journals New Labelling Technology for Molecular Probes Applied to the Ligation Detection Reaction–Universal Array System

2010 ◽  
Vol 47 (1) ◽  
pp. 1-8
Author(s):  
Andrea Lauri ◽  
Stefania Chessa ◽  
Marta Raschetti ◽  
Bianca Castiglioni ◽  
Paola Mariani ◽  
...  
Keyword(s):  
Molecular Probes ◽  
Ligation Detection Reaction ◽  
Universal Array
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