Polyphasic Taxonomy of Novel Actinobacteria Showing Macromolecule Degradation Potentials in Bigeum Island, Korea

2009 ◽  
Vol 59 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Syed G. Dastager ◽  
Ashok Pandey ◽  
Jae-Chan Lee ◽  
Wen-Jun Li ◽  
Chang-Jin Kim
Author(s):  
Shabnam Akhtar ◽  
Altaf Ahmad ◽  
Shipra R. Jha ◽  
Javed Ahmad

2005 ◽  
Vol 109 (5) ◽  
pp. 569-580 ◽  
Author(s):  
Lillane E. Petrini ◽  
Orlando Petrini

1986 ◽  
Vol 235 (3) ◽  
pp. 707-713 ◽  
Author(s):  
L H Rome ◽  
D F Hill

Lysosomal degradation of the carbohydrate portion of glycoproteins and glycosaminoglycans produces monosaccharides and sulphate, which must efflux from the lysosomes before re-entering biosynthetic pathways. We examined the degradation of glycoproteins and glycosaminoglycans by lysosomes isolated from cultured human diploid fibroblasts. Cells were grown for 24 h in medium containing [3H]glucosamine and [35S]sulphate. When lysosomes are isolated from these cells, they contain label primarily in macromolecules (glycoproteins and glycosaminoglycans). Glycoprotein degradation by isolated lysosomes was followed by measuring the release of tritiated sugars from macromolecules and efflux of these sugars from the organelles. Glycosaminoglycan degradation was monitored by the release of both tritiated sugars and [35S]sulphate. During macromolecule degradation, the total amounts of free [35S]sulphate, N-acetyl[3H]glucosamine and N-acetyl[3H]galactosamine found outside the lysosome parallels the amounts of these products released by degradation. The total degradation of glycoproteins and glycosaminoglycans by intact cultured cells was also examined. The lysosomal contribution to degradation was assessed by measuring inhibition by the lysosomotropic amine NH4Cl. After 48 h incubation, inhibition by NH4Cl exceeded 55% of glycoprotein and 72% of glycosaminoglycan degradation. Recycling of [3H]hexosamines and [35S]sulphate by intact cells was estimated by measuring the appearance of ‘newly synthesized’ radioactively labelled macromolecules in the medium. Sulphate does not appear to be appreciably recycled. N-Acetylglucosamine and N-acetylgalactosamine, on the other hand, are reutilized to a significant extent.


1998 ◽  
Vol 37 (4-5) ◽  
pp. 231-234 ◽  
Author(s):  
David R. Confer ◽  
Bruce E. Logan

Macromolecular (> 1,000 daltons) compounds such as proteins and polysaccharides can constitute a significant portion of dissolved organic carbon (DOC) in wastewater, but limited information is available on how these compounds are degraded in suspended and fixed-film biological wastewater treatment systems. Bacteria cannot assimilate intact macromolecules but must first hydrolyze them to monomers or small oligomers. Here, we summarize experiments performed in our laboratory which indicate that the enzymes responsible for hydrolysis are primarily those that remain attached to the cell. In biofilm cultures fed macromolecular substrates, for example, no more than 8% of total hydrolytic activity was found to be located in the cell-free bulk solution. These and other experiments support a generalized mechanism for macromolecule degradation by biofilms that features cell-associated hydrolysis, followed by the release of hydrolytic fragments back into bulk solution. The extent of fragment release is larger for proteins (bovine serum albumin) than for carbohydrates (dextrans).


2020 ◽  
Vol 129 (2) ◽  
pp. S129
Author(s):  
Karolina Pierzynowska ◽  
Lidia Gaffke ◽  
Magdalena Podlacha ◽  
Grzegorz Wegrzyn

1999 ◽  
Vol 22 (3) ◽  
pp. 360-365 ◽  
Author(s):  
Saipin Chaiyanan ◽  
Sitthipan Chaiyanan ◽  
Tim Maugel ◽  
Anwarul Huq ◽  
Frank T. Robb ◽  
...  

2014 ◽  
Vol 78 ◽  
pp. 175-341 ◽  
Author(s):  
N. Yilmaz ◽  
C.M. Visagie ◽  
J. Houbraken ◽  
J.C. Frisvad ◽  
R.A. Samson
Keyword(s):  

2017 ◽  
Vol 88 ◽  
pp. 37-135 ◽  
Author(s):  
A.J. Chen ◽  
V. Hubka ◽  
J.C. Frisvad ◽  
C.M. Visagie ◽  
J. Houbraken ◽  
...  

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