Cloning of the A Mating-Type Locus from Lepista nuda and Characterization of Its Genetic Structure

2015 ◽  
Vol 71 (6) ◽  
pp. 669-677 ◽  
Author(s):  
Chengbo Rong ◽  
Shuang Zhao ◽  
Dengjin Li ◽  
Lijuan Wang ◽  
Shouxian Wang ◽  
...  
Gene ◽  
2014 ◽  
Vol 535 (2) ◽  
pp. 184-190 ◽  
Author(s):  
Chun Hang Au ◽  
Man Chun Wong ◽  
Dapeng Bao ◽  
Meiyan Zhang ◽  
Chunyan Song ◽  
...  

2007 ◽  
Vol 8 (1) ◽  
pp. 111-120 ◽  
Author(s):  
LAURA CONDE-FERRÁEZ ◽  
CEES WAALWIJK ◽  
BLONDY B. CANTO-CANCHÉ ◽  
GERT H. J. KEMA ◽  
PEDRO W. CROUS ◽  
...  

2017 ◽  
Vol 49 (4) ◽  
pp. 373-385 ◽  
Author(s):  
Lars R. LUDWIG ◽  
Tina C. SUMMERFIELD ◽  
Janice M. LORD ◽  
Garima SINGH

AbstractIn the present study, we characterized the mating-type locus ofKnightiella splachnirima(Icmadophilaceae) using degenerate and inverse PCR techniques. We screened for the presence of both mating-type locus idiomorphs in DNA extracts of minuscule samples of haploid thalline tissue. We found that only one of the two idiomorphs was present in each sample, and the mating-type ratio (MAT1-1:MAT1-2) was very balanced, being 8:10 and 13:14 at local and global scales, respectively. This indicates that the species is probably self-incompatible and requires the presence of compatible mating partners for sexual reproduction (heterothallic mating system). Furthermore, we provide a mating-type screening protocol withK. splachnirimaspecific mating-type locus primers, which could serve as an essential tool for the conservation management of this rare Australasian endemic.


Gene ◽  
2013 ◽  
Vol 531 (2) ◽  
pp. 270-278 ◽  
Author(s):  
Lin Wu ◽  
Arend van Peer ◽  
Wenhua Song ◽  
Hong Wang ◽  
Mingjie Chen ◽  
...  

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