The genetic structure of the A mating-type locus of Lentinula edodes

Gene ◽  
2014 ◽  
Vol 535 (2) ◽  
pp. 184-190 ◽  
Author(s):  
Chun Hang Au ◽  
Man Chun Wong ◽  
Dapeng Bao ◽  
Meiyan Zhang ◽  
Chunyan Song ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mating Type ◽  
Lentinula Edodes ◽  
Type Locus ◽  
Mating Type Locus

Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating

Gene ◽  
2013 ◽  
Vol 531 (2) ◽  
pp. 270-278 ◽  
Author(s):  
Lin Wu ◽  
Arend van Peer ◽  
Wenhua Song ◽  
Hong Wang ◽  
Mingjie Chen ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mating Type ◽  
Lentinula Edodes ◽  
Type Locus ◽  
Mating Type Locus

scholarly journals Investigation of Mating Pheromone–Pheromone Receptor Specificity in Lentinula edodes

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 506
Author(s):  
Sinil Kim ◽  
Byeongsuk Ha ◽  
Minseek Kim ◽  
Hyeon-Su Ro
Keyword(s):  
Mating Type ◽  
Lentinula Edodes ◽  
Signal Pathway ◽  
Recombinant Yeast ◽  
Mating Pheromone ◽  
Type Locus ◽  
Pheromone Receptor ◽  
Mating Type Locus ◽  
Yeast Mating ◽  
Mating Signal

The B mating-type locus of Lentinula edodes, a representative edible mushroom, is highly complex because of allelic variations in the mating pheromone receptors (RCBs) and the mating pheromones (PHBs) in both the Bα and Bβ subloci. The complexity of the B mating-type locus, five Bα subloci with five alleles of RCB1 and nine PHBs and three Bβ subloci with 3 alleles of RCB2 and five PHBs, has led us to investigate the specificity of the PHB–RCB interaction because the interaction plays a key role in non-self-recognition. In this study, the specificities of PHBs to RCB1-2 and RCB1-4 from the Bα sublocus and RCB2-1 from the Bb sublocus were investigated using recombinant yeast strains generated by replacing STE2, an endogenous yeast mating pheromone receptor, with the L. edodes RCBs. Fourteen synthetic PHBs with C-terminal carboxymethylation but without farnesylation were added to the recombinant yeast cells and the PHB–RCB interaction was monitored by the expression of the FUS1 gene—a downstream gene of the yeast mating signal pathway. RCB1-2 (Bα2) was activated by PHB1 (4.3-fold) and PHB2 (2.1-fold) from the Bα1 sublocus and RCB1-4 (Bα4) was activated by PHB5 (3.0-fold) and PHB6 (2.7-fold) from the Bα2 sublocus and PHB13 (3.0-fold) from the Bα5 sublocus. In particular, PHB3 from Bβ2 and PHB9 from Bβ3 showed strong activation of RCB2-1 of the Bβ1 sublocus by 59-fold. The RCB–PHB interactions were confirmed in the monokaryotic S1–10 strain of L. edodes by showing increased expression of clp1, a downstream gene of the mating signal pathway and the occurrence of clamp connections after the treatment of PHBs. These results indicate that a single PHB can interact with a non-self RCB in a sublocus-specific manner for the activation of the mating pheromone signal pathways in L. edodes.

Cloning of the A Mating-Type Locus from Lepista nuda and Characterization of Its Genetic Structure

2015 ◽  
Vol 71 (6) ◽  
pp. 669-677 ◽  
Author(s):  
Chengbo Rong ◽  
Shuang Zhao ◽  
Dengjin Li ◽  
Lijuan Wang ◽  
Shouxian Wang ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus

scholarly journals rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation.

1981 ◽  
Vol 1 (10) ◽  
pp. 958-960 ◽  
Author(s):  
J Rine ◽  
G F Sprague ◽  
I Herskowitz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus ◽  
Type Information

Sporulation in Saccharomyces cerevisiae normally occurs only in MATa/MAT alpha diploids. We show that mutations in RME1 bypassed the requirements for both a and alpha mating type information in sporulation and therefore allowed MATa/MATa and MAT alpha/MAT alpha diploids to sporulate. RME1 was located on chromosome VII, between LEU1 and ADE6.

Transformation of protoplasted yeast cells is directly associated with cell fusion

1984 ◽  
Vol 4 (4) ◽  
pp. 771-778
Author(s):  
S Harashima ◽  
A Takagi ◽  
Y Oshima
Keyword(s):  
Cell Fusion ◽  
Mating Type ◽  
Nuclear Fusion ◽  
Yeast Cells ◽  
Nuclear Markers ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Protoplasts ◽  
Yeast Plasmids ◽  
Binominal Distribution

The frequency of cell fusion during transformation of yeast protoplasts with various yeast plasmids with a chromosome replicon (YRp or YCp) or 2 mu DNA (YEp) was estimated by two methods. In one method, a mixture of protoplasts of two haploid strains with identical mating type and complementary auxotrophic nuclear markers with or without cytoplasmic markers was transformed. When the number of various phenotypic classes of transformants for the nuclear markers was analyzed by equations derived from binominal distribution theory, the frequency of nuclear fusion among the transformants was 42 to 100% in transformations with the YRp or YCp plasmids and 28 to 39% with the YEp plasmids. In another method, a haploid bearing the sir mutation, which allows a diploid (or polyploid) homozygous for the MAT (mating type) locus to sporulate by the expression of the silent mating-type loci HML and HMR, was transformed with the plasmids. Sporulation ability was found in 43 to 95% of the transformants with the YRp or YCp plasmids, and 26 to 31% of the YEp transformants. When cytoplasmic mixing was included with the nuclear fusion, 96 to 100% of the transformants were found to be cell fusants. Based upon these observations, we concluded that transformation of yeast protoplasts is directly associated with cell fusion.

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