Extracting genotype information of Arabidopsis thaliana recombinant inbred lines from transcript profiles established with high-density oligonucleotide arrays

2017 ◽  
Vol 36 (12) ◽  
pp. 1871-1881
Author(s):  
Renate Schmidt ◽  
Anastassia Boudichevskaia ◽  
Hieu Xuan Cao ◽  
Sang He ◽  
Rhonda Christiane Meyer ◽  
...  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuka Miki ◽  
Kentaro Yoshida ◽  
Hiroyuki Enoki ◽  
Shoya Komura ◽  
Kazuyo Suzuki ◽  
...  

AbstractDue to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.


PLoS ONE ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. e4318 ◽  
Author(s):  
Sureshkumar Balasubramanian ◽  
Christopher Schwartz ◽  
Anandita Singh ◽  
Norman Warthmann ◽  
Min Chul Kim ◽  
...  

1992 ◽  
Vol 89 (4) ◽  
pp. 1477-1481 ◽  
Author(s):  
R. S. Reiter ◽  
J. G. Williams ◽  
K. A. Feldmann ◽  
J. A. Rafalski ◽  
S. V. Tingey ◽  
...  

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Zhiqiang Zhou ◽  
Chaoshu Zhang ◽  
Yu Zhou ◽  
Zhuanfang Hao ◽  
Zhenhua Wang ◽  
...  

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