scholarly journals GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuka Miki ◽  
Kentaro Yoshida ◽  
Hiroyuki Enoki ◽  
Shoya Komura ◽  
Kazuyo Suzuki ◽  
...  
Keyword(s):  
Flowering Time ◽  
Genetic Markers ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Aegilops Tauschii ◽  
Amplicon Sequencing ◽  
Inbred Lines ◽  
High Density ◽  
D Genome ◽  
Triticeae Species

AbstractDue to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.

Application of Aegilops tauschii–Triticum aestivum recombinant inbred lines for grain protein content quantitative trait loci detection and wheat improvement

2020 ◽  
Vol 100 (4) ◽  
pp. 425-434
Author(s):  
Yarui Su ◽  
Pingan Liao ◽  
Danyang Song ◽  
Shiquan Huang ◽  
Jie He ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Quantitative Trait Loci ◽  
Protein Content ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Aegilops Tauschii ◽  
Grain Protein Content ◽  
Inbred Lines ◽  
Quality Traits

Grain protein content (GPC) is an important nutritional quality trait of wheat. Aegilops tauschii Coss. is a progenitor of common wheat and has been shown to have high GPC. The objective of this study was to identify quantitative trait loci (QTL) for GPC using A. tauschii–Triticum aestivum L. recombinant lines. An advanced BC2F6 population (112 lines) containing A. tauschii segments was developed using synthetic octaploid wheat (hexaploid wheat Zhoumai 18 × A. tauschii T093), which displayed significant phenotype variances. Two quality traits, GPC and wet gluten, and four yield-related traits, thousand kernel weight, spikelet number per plant, grain number per spike, and grain weight per spike, were evaluated. The results show that the mean GPCs of these lines were significantly higher than those of Zhoumai 18. Correlation and mapping analyses indicated that quality traits were weakly negatively correlated with yield traits. Furthermore, 16 A. tauschii-derived QTL for GPC were detected in the recombinant inbred lines, and four stable QTL that have no significant negative effects on yield and are located within the same marker interval were detected in both environments. Additionally, high-protein, high-yield lines 150228 and 150368 with stable QTL were obtained, and both can be directly utilised for fine mapping of the GPC genes and molecular marker–assisted selection to achieve synergistic improvement of wheat yield and protein content.

Characterization of flowering time response among recombinant inbred lines of WAB638-1/PRIMAVERA rice under reproductive stage drought stress

2021 ◽  
pp. 1-8
Author(s):  
Kehinde A. Adeboye ◽  
Olusegun A. Oduwaye ◽  
Isaac O. Daniel ◽  
Mamadou Fofana ◽  
Mande Semon
Keyword(s):  
Drought Stress ◽  
Flowering Time ◽  
Recombinant Inbred ◽  
Root Length ◽  
Recombinant Inbred Lines ◽  
Block Design ◽  
Field Trials ◽  
Inbred Lines ◽  
Reproductive Stage ◽  
Time Response

Abstract Ninety-eight high-yielding recombinant inbred lines (RILs) of WAB638-1/PRIMAVERA rice were evaluated with the parents under reproductive stage drought stress. The study aimed at characterizing flowering time response under drought stress and consequently classifying the genetic resources for efficient use in breeding programmes. Two field trials were conducted during the 2016 and 2017 dry seasons using the randomized complete block design with two replications. In 2016, 12 RILs were evaluated with the parents for the rooting attributes under drought stress and well-watered treatments. Analysis of variance revealed a significant (P < 0.05) variation among the genotypes for the traits evaluated. Drought stress reduced most of the characters in this study, including grain yield. However, the inbred lines exhibited considerable tolerance to drought stress as indicated by yield-related stress indexes, including stress susceptibility and drought tolerance indexes. Delayed flowering (FD) was recorded in 75 genotypes (including WAB638-1), while 25 genotypes (including PRIMAVERA) had no delay under drought stress compared to the control. The genotypes were grouped into flowering delay (D) genotypes (FD > 1 d) and no delay (N) genotypes (FD < 1 d), which significantly differ (P ⩽ 0.05) for numbers of days to 50% flowering. The flowering delay genotypes may be suitable for intermittent drought, while genotypes with little or no delay may be selected for terminal drought conditions. There was a significant correlation (r > 0.5) between the root length and number of days to 50% flowering. The study indicated that root parameters, such as the root length, may contribute to the drought adaptation mechanisms of the RILs.

scholarly journals Genetic dissection of maize plant architecture with an ultra-high density bin map based on recombinant inbred lines

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Zhiqiang Zhou ◽  
Chaoshu Zhang ◽  
Yu Zhou ◽  
Zhuanfang Hao ◽  
Zhenhua Wang ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Plant Architecture ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
High Density ◽  
Maize Plant ◽  
Genetic Dissection ◽  
Bin Map

scholarly journals Construction of a High-density AFLP and SSR Map Using Recombinant Inbred Lines of Cultivated × Weedy Soybean

2003 ◽  
Vol 53 (4) ◽  
pp. 335-344 ◽  
Author(s):  
Pilson Choi ◽  
Yoshiro Mano ◽  
Atsuko Ishikawa ◽  
Masashi Odashima ◽  
Taishi Umezawa ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
High Density

Diverse sources of resistance to Spodoptera litura (F.) in groundnut

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
M. A. Saleem ◽  
G. K. Naidu ◽  
H. L. Nadaf ◽  
P. S. Tippannavar
Keyword(s):  
Recombinant Inbred ◽  
Spodoptera Litura ◽  
Hot Spot ◽  
Recombinant Inbred Lines ◽  
Insect Pest ◽  
Inbred Lines ◽  
Leaf Damage ◽  
Botanical Variety ◽  
Resistant Genotypes ◽  
Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

Sign in / Sign up

Export Citation Format

Share Document