Biochemical properties of range and forest soils in Mediterranean mountain environments

The 4-nitrophenyl phosphate method for acid phosphomonoesterase (EC 3.1.3.2; acid orthophosphoric monoester phosphohydrolase) determination was slightly modified to increase the sensitivity and the stability of the 4-nitrophenol coloured complex in samples rich in humic substances and easily soluble organic compounds. Based on an approach used for mycorrhizal roots, the new analytical protocol was tested on samples taken from a single forest site with a large variation in soil types. Distinctive properties of forest soils and the accuracy/repeatability of the optimised technique were considered in the selection of the most appropriate analytical steps. Finally, the acid phosphomonoesterase activity was expressed in μg of 4-nitrophenol/g fresh soil recalculated dry matter/h released after hydrolysis of 4-nitrophenyl phosphate in sodium tetraborate/succinic acid buffer at pH 4.8. The modified method proposed was statistically compared with other procedures and the results obtained were found to give merit to these slight modifications. The rationale behind the slight modifications and their comparative significance was reported. Due to high soil complexity, it is suggested that a single laboratory measurement of chemical and biochemical properties is of limited application with forest soil and can be only one component of a broadly focused ecological analysis.

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Partial purification and some biochemical properties of non-specific alkaline phosphatase in germinating chick-pea (Cicer arietinum) seeds

Physiologia Plantarum ◽

10.1034/j.1399-3054.1990.800122.x ◽

1990 ◽

Vol 80 (1) ◽

pp. 136-142

Author(s):

Trinidad Angosto ◽

Angel Matilla

Keyword(s):

Alkaline Phosphatase ◽

Cicer Arietinum ◽

Biochemical Properties ◽

Partial Purification ◽

Chick Pea ◽

Specific Alkaline Phosphatase

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Binding of 131I-Labeled Tissue-Type Plasminogen Activator on De-Endothelialized Lesions in Rabbits

Nuklearmedizin ◽

10.1055/s-0038-1628893 ◽

1987 ◽

Vol 26 (05) ◽

pp. 224-228 ◽

Cited By ~ 2

Author(s):

Y. Isaka ◽

H. Etani ◽

K. Kimura ◽

S. Yoneda ◽

T. Kamada ◽

...

Keyword(s):

Plasminogen Activator ◽

Abdominal Aorta ◽

Half Life ◽

Balloon Catheter ◽

Biochemical Properties ◽

Tissue Type ◽

Fibrin Deposition ◽

Tissue Type Plasminogen Activator ◽

High Affinity ◽

Type Plasminogen Activator

Tissue-type plasminogen activator (t-PA) which has a high affinity for fibrin in the clot, was labeled with 131I by the iodogen method, and its binding to de-endothelialized lesions in the rabbit was measured to assess the detectability of thrombi. The de-endothelialized lesion was induced in the abdominal aorta with a Fogarty 4F balloon catheter. Two hours after the de-endothelialization, 131I-labeled t-PA (125 ± 46 μCi) was injected intravenously. The initial half-life of the agent in blood (n = 12) was 2.9 ± 0.4 min. The degree of binding of 131I-labeled t-PA to the de-endothelialized lesion was evaluated at 15 min (n = 6) or at 30 min (n = 6) after injection of the agent. In spite of the retention of the biochemical properties of 131I-labeled t-PA and the presence of fibrin deposition at the de-endothelialized lesion, the binding of t-PA to the lesion was not sufficiently strong. Lesion-to-control ratios (cpm/g/cpm/g) were 1.65 ± 0.40 (at 15 min) and 1.39 ± 1.31 (at 30 min), and lesion-to-blood ratios were 1.39 ± 0.32 (at 15 min) and 1.36 ± 0.23 (at 30 min). These results suggest that radiolabeled t-PA may be inappropriate as a radiopharmaceutical for the scintigraphic detection of a pre-existing thrombotic lesion.

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Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

Agricultural science and practice ◽

10.15407/agrisp3.01.043 ◽

2016 ◽

Vol 3 (1) ◽

pp. 43-48 ◽

Cited By ~ 1

Author(s):

V. Patyka ◽

L. Butsenko ◽

L. Pasichnyk

Keyword(s):

Erwinia Amylovora ◽

Anaerobic Bacteria ◽

Biochemical Properties ◽

Test System ◽

Phytopathogenic Bacteria ◽

Gram Negative ◽

Test Systems ◽

Facultatively Anaerobic ◽

Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

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