Early detection of Mycobacterium tuberculosis complex in BACTEC MGIT cultures using nucleic acid amplification

2016 ◽  
Vol 35 (6) ◽  
pp. 977-984 ◽  
Author(s):  
S. Y. Lin ◽  
S. C. Hwang ◽  
Y. C. Yang ◽  
C. F. Wang ◽  
Y. H. Chen ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Early Detection ◽  
Mycobacterium Tuberculosis Complex ◽  
Nucleic Acid Amplification ◽  
Tuberculosis Complex

scholarly journals Evaluation of Three Nucleic Acid Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens

1999 ◽  
Vol 37 (6) ◽  
pp. 1932-1934 ◽  
Author(s):  
S. X. Wang ◽  
L. Tay
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Mycobacterium Tuberculosis Complex ◽  
Direct Detection ◽  
Nucleic Acid Amplification ◽  
Smear Microscopy ◽  
Direct Test ◽  
Tuberculosis Complex ◽  
Chain Reaction ◽  
Respiratory Specimens

Two hundred thirty respiratory specimens from 230 patients were analyzed by using COBAS AMPLICOR PCR, Amplified Mycobacterium tuberculosis Direct Test, and ligase chain reaction methods. Results were compared with those of smear microscopy and radiometric culture (Bactec) methods. No significant differences were observed among the results of the three methods, which are acceptable for direct detection of M. tuberculosis complex in respiratory specimens.

scholarly journals The rational use of nucleic acid amplification testing for the Mycobacterium tuberculosis complex

2004 ◽  
Vol 25 (4) ◽  
pp. 28
Author(s):  
Richard Lumb
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Clinical Significance ◽  
Mycobacterium Bovis ◽  
Mycobacterium Tuberculosis Complex ◽  
Nucleic Acid Amplification ◽  
Tuberculosis Complex ◽  
Rational Use ◽  
Genus Mycobacterium ◽  
Mycobacterium Microti

On the basis of clinical significance, Mycobacterium tuberculosis is the most important member of the genus Mycobacterium. It is closely related genetically to Mycobacterium bovis, M. africanum, Mycobacterium microti, Mycobacterium bovis BCG (the bacillus of Calmette-Guerin) and the recently described Mycobacterium tuberculosis subspecies Canetti. Together they are termed the Mycobacterium tuberculosis complex (MTBC).

scholarly journals 1633. Improving the diagnosis of extra-pulmonary tuberculosis (EPTB): experience from north India

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S808-S808
Author(s):  
Anchal Sharma ◽  
Kusum Sharma ◽  
Manish Modi ◽  
Aman Sharma
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Rural Setting ◽  
Tuberculosis Complex ◽  
Extra Pulmonary Tuberculosis ◽  
Resource Poor ◽  
Extra Pulmonary

Abstract Background Rapid and accurate diagnosis of extra-pulmonary tuberculosis (EPTB) is imperative for early treatment and better patient outcome. Loop-mediated Isothermal Amplification (LAMP) is a promising nucleic-acid amplification assay. LAMP assay could be carried out in simple water bath under isothermal conditions in 60 minutes, and can be performed in any laboratory even in rural setting in resource poor endemic countries. We evaluated LAMP assay using two different target regions LAMP primers specific for Mycobacterium tuberculosis complex for the diagnosis of EPTB. Methods LAMP assay using 6 primers (each for IS6110 and IS1081) specific for Mycobacterium tuberculosis complex were performed on patients suspected of EPTB on various EPTB samples(CSF, Synovial fluid, Lymaphnode and tissue biopsies and various other samples) of 150 patients (50 confirmed, 100 suspected) Clinically suspected of EPTB and 100 non-TB control subjects. Results Overall LAMP test (using any of the two targets) had sensitivity and specificity of 96% and 100% for confirmed (50 culture positive) EPTB cases. In 100 clinically suspected but unconfirmed EPTB cases, LAMP was positive in 87 out of 100 cases (87%). Sensitivity of IS6110 LAMP, 1S1081 LAMP and IS6110 PCR for clinically suspected cases was 78 (78%), 84 (84%) and 70 (70%), respectively. In total 150 EPTB patients, the overall sensitivity of microscopy, culture, IS6110 PCR, IS6110 LAMP, 1081 LAMP and the LAMP test (if any of the two targets were used) were 4%, 33.3%, 74.6%, 82.66%, 87% and 92%, respectively. Specificity of all the tests was 100%. There were 8 cases which were missed by IS6110 LAMP and 2 cases by 1081 LAMP. Conclusion LAMP assay using two targets is a promising technique for rapid diagnosis of EPTB in 60 minutes especially in a resource poor setting who are still battling with this deadly disease. Disclosures All Authors: No reported disclosures

scholarly journals Trends in Testing for Mycobacterium tuberculosis Complex From US Public Health Laboratories, 2009–2013

2016 ◽  
Vol 132 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Frances Tyrrell ◽  
Cortney Stafford ◽  
Mitchell Yakrus ◽  
Monica Youngblood ◽  
Andrew Hill ◽  
...  
Keyword(s):  
Public Health ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
The United States ◽  
Drug Susceptibility ◽  
Turnaround Time ◽  
Nucleic Acid Amplification ◽  
Tuberculosis Complex ◽  
Complex Detection ◽  
Susceptibility Tests

Objective: We investigated data from US public health laboratories funded through the Centers for Disease Control and Prevention’s Tuberculosis Elimination and Laboratory Cooperative Agreement to document trends and challenges in meeting national objectives in tuberculosis (TB) laboratory diagnoses. Methods: We examined data on workload and turnaround time from public health laboratories’ progress reports during 2009-2013. We reviewed methodologies, laboratory roles, and progress toward rapid detection of Mycobacterium tuberculosis complex through nucleic acid amplification (NAA) testing. We compared selected data with TB surveillance reports to estimate public health laboratories’ contribution to national diagnostic services. Results: During the study period, culture and drug susceptibility tests decreased, but NAA testing increased. Public health laboratories achieved turnaround time benchmarks for drug susceptibility tests at lower levels than for acid-fast bacilli smear and identification from culture. NAA positivity in laboratories among surveillance-reported culture-positive TB cases increased from 26.6% (2355 of 8876) in 2009 to 40.0% (2948 of 7358) in 2013. Public health laboratories provided an estimated 50.9% (4285 of 8413 in 2010) to 57.2% (4210 of 7358 in 2013) of culture testing and 88.3% (6822 of 7727 in 2011) to 94.4% (6845 of 7250 in 2012) of drug susceptibility tests for all US TB cases. Conclusions: Public health laboratories contribute substantially to TB diagnoses in the United States. Although testing volumes mostly decreased, the increase in NAA testing indicates continued progress in rapid M tuberculosis complex detection.

scholarly journals Comparative Evaluation of the New Gen-Probe Mycobacterium tuberculosis Amplified Direct Test and the Semiautomated Abbott LCx Mycobacterium tuberculosis Assay for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory and Extrapulmonary Specimens

1998 ◽  
Vol 36 (12) ◽  
pp. 3601-3604 ◽  
Author(s):  
Claudio Piersimoni ◽  
Annapaola Callegaro ◽  
Claudio Scarparo ◽  
Valeria Penati ◽  
Domenico Nista ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Direct Detection ◽  
Extrapulmonary Tuberculosis ◽  
Nucleic Acid Amplification ◽  
Tuberculosis Complex ◽  
Predictive Values ◽  
Clinical Specimens ◽  
Respiratory Specimens ◽  
Level Of Agreement

Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were compared with those of acid-fast staining and culture. The combination of culture and clinical diagnosis was considered to be the “gold standard.” Seventy specimens were from patients with pulmonary tuberculosis and 28 specimens were from patients with extrapulmonary tuberculosis. After resolution of discrepant results, the overall sensitivities, specificities, and positive and negative predictive values for respiratory specimens were 92.8, 99.4, 98.5, and 97%, respectively, for AMTDII and 75.7, 98.8, 96.4, and 90.5%, respectively, for LCx. With extrapulmonary specimens, the overall sensitivities, specificities, and positive and negative predictive values were 78.6, 99.3, 95.6, and 96.2%, respectively, for AMTDII and 53.6, 99.3, 93.7, and 92.1%, respectively, for LCx. The level of agreement between AMTDII and LCx assay results was 78.2%. We conclude that although both nucleic acid amplification methods are rapid and specific for the detection of MTB in clinical specimens, AMTDII is significantly more sensitive than LCx with both respiratory (P = 0.005) and extrapulmonary (P = 0.048) specimens.

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