Effect of Unsymmetrical Dimethylhydrazine on Isolated Heart and Lymphatic Vessels

2022 ◽  
Author(s):  
O. V. Nechaykina ◽  
D. S. Laptev ◽  
S. G. Petunov ◽  
D. V. Bobkov
Keyword(s):  
Isolated Heart ◽  
Lymphatic Vessels ◽  
Unsymmetrical Dimethylhydrazine

Effect of unsymmetrical dimethylhydrazine on isolated heart and lymphatic vessels

2021 ◽  
Vol 172 (9) ◽  
pp. 283-286
Author(s):  
O. V. Nechaykina ◽  
◽  
D. S. Laptev ◽  
S. G. Petunov ◽  
D. V. Bobkov ◽  
...  
Keyword(s):  
Isolated Heart ◽  
Lymphatic Vessels ◽  
Unsymmetrical Dimethylhydrazine

scholarly journals The effect of β-endorphin on the functional parameters of the isolated heart and lymphatic vessels of the white rat

2019 ◽  
Vol 11 (2) ◽  
pp. 43-48 ◽  
Author(s):  
Olga V. Nechaykina ◽  
Denis S. Laptev ◽  
Sergei G. Petunov ◽  
Andrei S. Radilov
Keyword(s):  
Opioid Receptors ◽  
Rat Heart ◽  
Isolated Heart ◽  
Experimental Studies ◽  
Lymphatic Vessels ◽  
Isolated Rat Heart ◽  
K Channel ◽  
Channel Blockers ◽  
K Channels ◽  
Isolated Rat

Objective. The work is devoted to the comparison of the mechanisms of β-endorphin action in isolated rat heart and lymphatic vessels. Materials and methods. The experiments were performed using the Langendorff System perfusion device (Panlab, Spain) and the multichannel wire myograph 620M (DMT). During the study, selective opioid receptor blockers, K + channel blockers were used. Conclusion. In the course of experimental studies it was found that the most likely target for β-endorphin in an isolated rat heart are δ-opioid receptors, in isolated lymphatic vessels of a rat - μ- and δ-opioid receptors. The inhibitory effect of β-endorphin in the heart muscle is associated with stimulation of ATP-sensitive K + channels. In isolated lymphatic vessels, the effect of β-endorphin is realized through the activation of both potential-dependent and ATP-sensitive K+ channels.

Confocal imaging of calcium in intact ventricular muscle provides a new view of excitation-contraction coupling

1996 ◽  
Vol 54 ◽  
pp. 738-739
Author(s):  
W.G. Wier
Keyword(s):  
Local Control ◽  
Cardiac Tissue ◽  
Cell Function ◽  
Isolated Heart ◽  
Cardiac Cells ◽  
Confocal Imaging ◽  
Ion Concentration ◽  
Heart Cell ◽  
Free Calcium ◽  
Heart Cells

A fundamentally new understanding of cardiac excitation-contraction (E-C) coupling is being developed from recent experimental work using confocal microscopy of single isolated heart cells. In particular, the transient change in intracellular free calcium ion concentration ([Ca2+]i transient) that activates muscle contraction is now viewed as resulting from the spatial and temporal summation of small (∼ 8 μm3), subcellular, stereotyped ‘local [Ca2+]i-transients' or, as they have been called, ‘calcium sparks'. This new understanding may be called ‘local control of E-C coupling'. The relevance to normal heart cell function of ‘local control, theory and the recent confocal data on spontaneous Ca2+ ‘sparks', and on electrically evoked local [Ca2+]i-transients has been unknown however, because the previous studies were all conducted on slack, internally perfused, single, enzymatically dissociated cardiac cells, at room temperature, usually with Cs+ replacing K+, and often in the presence of Ca2-channel blockers. The present work was undertaken to establish whether or not the concepts derived from these studies are in fact relevant to normal cardiac tissue under physiological conditions, by attempting to record local [Ca2+]i-transients, sparks (and Ca2+ waves) in intact, multi-cellular cardiac tissue.

Infrared fluorescence lymphography in experimental and clinical practice

2018 ◽  
Vol 17 (2) ◽  
pp. 84-91 ◽  
Author(s):  
G. V. Papayan ◽  
A. L. Akopov ◽  
P. A. Antonyan ◽  
A. A. Ilin ◽  
N. N. Petrishchev
Keyword(s):  
Lymph Nodes ◽  
Near Infrared ◽  
Lymphatic System ◽  
Lymphatic Vessels ◽  
Diagnostic System ◽  
Biological Tissues ◽  
Peritumoral Injection ◽  
Intradermal Administration ◽  
Nir Fluorescence ◽  
Real Time Visualization

Introduction. Near infrared (NIR) fluorescent diagnostics is promising due to a deeper penetration into biological tissues. Material and methods. In experiments on rabbits and in clinical studies evaluation the lymphatic system with the use of the instrument complex FLUM-808 was analysed. Results. For visualization of the lymphatic vessels of the skin, the intradermal administration of ICG, dissolved in 20 % albumin in the order of 0.02 mg/ml, is optimal. Peritumoral injection of ICG allows visualizing sentinel lymph nodes in patients with lung cancer. Conclusions. The developed NIR fluorescence diagnostic system FLUM-808 allows to real time visualization of lymphatic vessels and lymph nodes.

Sign in / Sign up

Export Citation Format

Share Document