Isolation and functional characterization of a novel gene coding for flavonoid 3'-hydroxylase from globe artichoke

2014 ◽  
Vol 58 (3) ◽  
pp. 445-455 ◽  
Author(s):  
M. Palma ◽  
F. Fratianni ◽  
F. Nazzaro ◽  
M. Tucci
Keyword(s):  
Functional Characterization ◽  
Globe Artichoke ◽  
Novel Gene ◽  
Gene Coding

Identification and functional characterization of pfm, a novel gene involved in swimming motility of Pseudomonas aeruginosa

Gene ◽  
2007 ◽  
Vol 401 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
Fang Bai ◽  
Yingli Li ◽  
Haijing Xu ◽  
Huiming Xia ◽  
Tengfei Yin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Functional Characterization ◽  
Swimming Motility ◽  
Novel Gene

scholarly journals Functional characterization of Candida albicans Hos2 histone deacetylase

F1000Research ◽  
2014 ◽  
Vol 2 ◽  
pp. 238
Author(s):  
G Karthikeyan ◽  
Maneesh Paul-Satyaseela ◽  
Nachiappan Dhatchana Moorthy ◽  
Radha Gopalaswamy ◽  
Shridhar Narayanan
Keyword(s):  
Candida Albicans ◽  
Histone Deacetylase ◽  
Trichostatin A ◽  
Functional Characterization ◽  
Gene Coding ◽  
Mold And Yeast ◽  
Anti Fungal ◽  
Vaginal Thrush ◽  
Inhibition Studies

Candida albicans is a mucosal commensal organism capable of causing superficial (oral and vaginal thrush) infections in immune normal hosts, but is a major pathogen causing systemic and mucosal infections in immunocompromised individuals. Azoles have been very effective anti-fungal agents and the mainstay in treating opportunistic mold and yeast infections. Azole resistant strains have emerged compromising the utility of this class of drugs. It has been shown that azole resistance can be reversed by the co-administration of a histone deacetylase (HDAC) inhibitor, suggesting that resistance is mediated by epigenetic mechanisms possibly involving Hos2, a fungal deacetylase. We report here the cloning and functional characterization of HOS2 (HighOsmolarity Sensitive), a gene coding for fungal histone deacetylase from C. albicans. Inhibition studies showed that Hos2 is susceptible to pan inhibitors such as trichostatin A (TSA) and suberoylanilide hydroxamic acid (SAHA), but is not inhibited by class I inhibitors such as MS-275. This in vitro enzymatic assay, which is amenable to high throughput could be used for screening potent fungal Hos2 inhibitors that could be a potential anti-fungal adjuvant. Purified Hos2 protein consistently deacetylated tubulins, rather than histones from TSA-treated cells. Hos2 has been reported to be a putative NAD+ dependent histone deacetylase, a feature of sirtuins. We assayed for sirtuin activation with resveratrol and purified Hos2 protein and did not find any sirtuin activity.

scholarly journals Structural and Functional Characterization of Four Novel Fibrinogen Mutations in FGB Causing Congenital Fibrinogen Disorder

2022 ◽  
Vol 23 (2) ◽  
pp. 721
Author(s):  
Eliška Ceznerová ◽  
Jiřina Kaufmanová ◽  
Žofie Sovová ◽  
Jana Štikarová ◽  
Jan Loužil ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Abnormal Development ◽  
Screening Tests ◽  
Morphological Properties ◽  
Fiber Density ◽  
Reverse Phase ◽  
Gene Coding ◽  
Novel Variants ◽  
Fiber Thickness

Congenital fibrinogen disorders are caused by mutations in genes coding for fibrinogen and may lead to various clinical phenotypes. Here, we present a functional and structural analysis of 4 novel variants located in the FGB gene coding for fibrinogen Bβ chain-heterozygous missense BβY416C and BβA68S, homozygous nonsense BβY345*, and heterozygous nonsense BβW403* mutations. The cases were identified by coagulation screening tests and further investigated by various methods. Fibrin polymerization had abnormal development with decreased maximal absorbance in all patients. Plasmin-induced fibrin degradation revealed different lytic phases of BβY416C and BβW403* than those of the control. Fibrinopeptide cleavage measured by reverse phase high pressure liquid chromatography of BβA68S showed impaired release of fibrinopeptide B. Morphological properties, studied through scanning electron microscopy, differed significantly in the fiber thickness of BβY416C, BβA68S, and BβW403*, and in the fiber density of BβY416C and BβW403*. Finally, homology modeling of BβA68S showed that mutation caused negligible alternations in the protein structure. In conclusion, all mutations altered the correct fibrinogen function or structure that led to congenital fibrinogen disorders.

scholarly journals HvPAA1 Encodes a P-Type ATPase, a Novel Gene for Cadmium Accumulation and Tolerance in Barley (Hordeum vulgare L.)

2019 ◽  
Vol 20 (7) ◽  
pp. 1732 ◽  
Author(s):  
Xin-Ke Wang ◽  
Xue Gong ◽  
Fangbin Cao ◽  
Yizhou Wang ◽  
Guoping Zhang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Functional Characterization ◽  
Cadmium Accumulation ◽  
Hordeum Vulgare L ◽  
Dh Population ◽  
Cd Accumulation ◽  
Novel Gene ◽  
Cd Tolerance ◽  
P Type

The identification of gene(s) that are involved in Cd accumulation/tolerance is vital in developing crop cultivars with low Cd accumulation. We developed a doubled haploid (DH) population that was derived from a cross of Suyinmai 2 (Cd-sensitive) × Weisuobuzhi (Cd-tolerant) to conduct quantitative trait loci (QTL) mapping studies. We assessed chlorophyll content, traits that are associated with development, metal concentration, and antioxidative enzyme activity in DH population lines and parents under control and Cd stress conditions. A single QTL, designated as qShCd7H, was identified on chromosome 7H that was linked to shoot Cd concentration; qShCd7H explained 17% of the phenotypic variation. Comparative genomics, map-based cloning, and gene silencing were used in isolation, cloning, and functional characterization of the candidate gene. A novel gene HvPAA1, being related to shoot Cd concentration, was identified from qShCd7H. Sequence comparison indicated that HvPAA1 carried seven domains with an N-glycosylation motif. HvPAA1 is predominantly expressed in shoots. Subcellular localization verified that HvPAA1 is located in plasma membrane. The silencing of HvPAA1 resulted in growth inhibition, greater Cd accumulation, and a significant decrease in Cd tolerance. We conclude HvPAA1 is a novel plasma membrane-localized ATPase that contributes to Cd tolerance and accumulation in barley. The results provide us with new insights that may aid in the screening and development of Cd-tolerant and low-Cd-accumulation crops.

scholarly journals TworsaMhomologues encode central regulatory elements modulating quorum sensing expression inBurkholderia thailandensis

2017 ◽  
Author(s):  
Servane Le Guillouzer ◽  
Marie-Christine Groleau ◽  
Eric Déziel
Keyword(s):  
Quorum Sensing ◽  
Functional Characterization ◽  
Gene Clusters ◽  
Homoserine Lactone ◽  
Regulatory Elements ◽  
Burkholderia Thailandensis ◽  
Gene Coding ◽  
Genes Expression ◽  
In The Wild

AbstractThe bacteriumBurkholderia thailandensispossesses three conservedN-acyl-L-homoserine lactone (AHL) quorum sensing (QS) systems designated BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). These QS-systems are associated with the biosynthesis ofN-octanoyl-homoserine lactone (C8-HSL),N-3-hydroxy-decanoyl-homoserine lactone (3OHC10-HSL), as well asN-3-hydroxy-octanoyl-homoserine lactone (3OHC8-HSL), which are produced by the LuxI-type synthase BtaI1, BtaI2, and BtaI3, and modulated by the LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3. BothbtaR1/btaI1andbtaR2/btaI2gene clusters contain an additional gene that is conserved in theBurkholderiagenus, homologous to a gene coding for the negative AHL biosynthesis modulatory protein RsaM originally identified in the phytopathogenPseudomonas fuscovaginae, and hence designatedrsaM1andrsaM2. We have characterized the function of these tworsaMhomologues and demonstrated their involvement in the regulation of AHLs biosynthesis inB. thailandensisstrain E264. We measured the production of C8-HSL, 3OHC10-HSL, and 3OHC8-HSL by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in the wild-type strain and in thersaM1-andrsaM2-mutants, and monitored the transcription ofbtaI1,btaI2, andbtaI3 using chromosomal mini-CTX-luxtranscriptional reporters. The expression ofbtaR1,btaR2, andbtaR3 was also measured by quantitative everse-transcription PCR (qRT-PCR). We demonstrate that the QS-1 system is repressed by RsaM1, whereas RsaM2 principally represses the QS-2 system. We also found that bothrsaM1andrsaM2are QS-controlled, as well as negatively auto-regulated. We conclude that RsaM1 and RsaM2 are an integral part of the QS modulatory circuitry ofB. thailandensis, and play a major role in the hierarchical and homeostatic organization of the QS-1, QS-2, and QS-3 systems.ImportanceQuorum sensing (QS) is a global regulatory mechanism of genes expression depending on bacterial density. QS is commonly involved in the coordination of genes expression associated with the establishment of host-pathogen interactions and acclimatization to the environment. We present the functional characterization of the tworsaMhomologues designatedrsaM1andrsaM2in the regulation of the multiple QS systems coexisting in the non-pathogenic bacteriumBurkholderia thailandensis, widely used as a model system for the study of the pathogenBurkholderia pseudomallei. We found that inactivation of thesersaMhomologues, which are clustered with the other QS genes, profoundly affects the QS regulatory circuity ofB. thailandensis. It is proposed that these genes code for QS repressors and we conclude that they constitute essential regulatory components of the QS modulatory network ofB. thailandensis, and provide additional layers of regulation to modulate the expression of QS-controlled genes, including those encoding virulence/survival factors and linked to environmental adaptation inB. pseudomallei.

scholarly journals Structural and functional characterization of a novel gene, Hc-daf-22, from the strongylid nematode Haemonchus contortus

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Xiaolu Guo ◽  
Hongli Zhang ◽  
Xiuping Zheng ◽  
Qianjin Zhou ◽  
Yi Yang ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Functional Characterization ◽  
Novel Gene
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