Functional characterization of a novel gene, Hc-dhs-28 and its role in protecting the host after Haemonchus contortus infection through regulation of diapause formation

2020 ◽  
Vol 50 (12) ◽  
pp. 945-957
Author(s):  
Yi Yang ◽  
Xiaolu Guo ◽  
Xueqiu Chen ◽  
Jingru Zhou ◽  
Fei Wu ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Functional Characterization ◽  
Novel Gene

scholarly journals Structural and functional characterization of a novel gene, Hc-daf-22, from the strongylid nematode Haemonchus contortus

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Xiaolu Guo ◽  
Hongli Zhang ◽  
Xiuping Zheng ◽  
Qianjin Zhou ◽  
Yi Yang ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Functional Characterization ◽  
Novel Gene

Identification and functional characterization of pfm, a novel gene involved in swimming motility of Pseudomonas aeruginosa

Gene ◽  
2007 ◽  
Vol 401 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
Fang Bai ◽  
Yingli Li ◽  
Haijing Xu ◽  
Huiming Xia ◽  
Tengfei Yin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Functional Characterization ◽  
Swimming Motility ◽  
Novel Gene

scholarly journals HvPAA1 Encodes a P-Type ATPase, a Novel Gene for Cadmium Accumulation and Tolerance in Barley (Hordeum vulgare L.)

2019 ◽  
Vol 20 (7) ◽  
pp. 1732 ◽  
Author(s):  
Xin-Ke Wang ◽  
Xue Gong ◽  
Fangbin Cao ◽  
Yizhou Wang ◽  
Guoping Zhang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Functional Characterization ◽  
Cadmium Accumulation ◽  
Hordeum Vulgare L ◽  
Dh Population ◽  
Cd Accumulation ◽  
Novel Gene ◽  
Cd Tolerance ◽  
P Type

The identification of gene(s) that are involved in Cd accumulation/tolerance is vital in developing crop cultivars with low Cd accumulation. We developed a doubled haploid (DH) population that was derived from a cross of Suyinmai 2 (Cd-sensitive) × Weisuobuzhi (Cd-tolerant) to conduct quantitative trait loci (QTL) mapping studies. We assessed chlorophyll content, traits that are associated with development, metal concentration, and antioxidative enzyme activity in DH population lines and parents under control and Cd stress conditions. A single QTL, designated as qShCd7H, was identified on chromosome 7H that was linked to shoot Cd concentration; qShCd7H explained 17% of the phenotypic variation. Comparative genomics, map-based cloning, and gene silencing were used in isolation, cloning, and functional characterization of the candidate gene. A novel gene HvPAA1, being related to shoot Cd concentration, was identified from qShCd7H. Sequence comparison indicated that HvPAA1 carried seven domains with an N-glycosylation motif. HvPAA1 is predominantly expressed in shoots. Subcellular localization verified that HvPAA1 is located in plasma membrane. The silencing of HvPAA1 resulted in growth inhibition, greater Cd accumulation, and a significant decrease in Cd tolerance. We conclude HvPAA1 is a novel plasma membrane-localized ATPase that contributes to Cd tolerance and accumulation in barley. The results provide us with new insights that may aid in the screening and development of Cd-tolerant and low-Cd-accumulation crops.

scholarly journals Molecular Cloning and Functional Characterization of a Novel Gene Encoding Human Prostaglandin Carrier, hPrC

2011 ◽  
Vol 131 (10) ◽  
pp. 1493-1501
Author(s):  
Yasuna KOBAYASHI ◽  
Junko NOJIMA ◽  
Masayuki OHBAYASHI ◽  
Noriko KOHYAMA ◽  
Toshinori YAMAMOTO
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
Gene Encoding ◽  
Novel Gene

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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