scholarly journals Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

2016 ◽  
Vol 68 (6) ◽  
pp. 2345-2354 ◽  
Author(s):  
Ulvi K. Gursoy ◽  
Mervi Gursoy ◽  
Eija Könönen ◽  
Herman O. Sintim ◽  
Veli-Jukka Uitto ◽  
...  
Keyword(s):  
Culture Model ◽  
Keratinocyte Culture

scholarly journals Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers

2014 ◽  
Vol 369 (1652) ◽  
pp. 20130502 ◽  
Author(s):  
Mu Li ◽  
Emily Zeringer ◽  
Timothy Barta ◽  
Jeoffrey Schageman ◽  
Angie Cheng ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cell Communication ◽  
Cell Culture Model ◽  
Culture Model ◽  
Wide Range ◽  
Abnormal Cells ◽  
A Cell ◽  
Invasive Diagnostics ◽  
Serum And Urine

Exosomes are tiny vesicles (30–150 nm) constantly secreted by all healthy and abnormal cells, and found in abundance in all body fluids. These vesicles, loaded with unique RNA and protein cargo, have a wide range of biological functions, including cell-to-cell communication and signalling. As such, exosomes hold tremendous potential as biomarkers and could lead to the development of minimally invasive diagnostics and next generation therapies within the next few years. Here, we describe the strategies for isolation of exosomes from human blood serum and urine, characterization of their RNA cargo by sequencing, and present the initial data on exosome labelling and uptake tracing in a cell culture model. The value of exosomes for clinical applications is discussed with an emphasis on their potential for diagnosing and treating neurodegenerative diseases and brain cancer.

scholarly journals Generation of Glioblastoma Patient-Derived Intracranial Xenografts for Preclinical Studies

2020 ◽  
Vol 21 (14) ◽  
pp. 5113
Author(s):  
Amber E. Kerstetter-Fogle ◽  
Peggy L. R. Harris ◽  
Susann M. Brady-Kalnay ◽  
Andrew E. Sloan
Keyword(s):  
Cultured Cells ◽  
Clonal Selection ◽  
Previous Treatment ◽  
Growth Patterns ◽  
Phenotypic Characterization ◽  
Genomic Signatures ◽  
Culture Model ◽  
Passage Number ◽  
Human Gbm

Glioblastoma multiforme (GBM) is the most malignant primary brain cancer affecting adults. Therapeutic options for GBM have remained the same for over a decade with no significant improvement. Many therapies that are successful in culture have failed in patients, likely due to the complex microenvironment in the brain, which has yet to be reproduced in any culture model. Furthermore, the high passage number of cultured cells and clonal selection fail to recapitulate the molecular and genomic signatures of GBM. We have established orthotopic patient-derived xenografts (PDX) from 37 GBM patients with human GBM. Of the 69 patient samples analyzed, we were successful in passaging 37 lines three or more generations (53.6%). After phenotypic characterization of the xenografted tumor tissue, two different growth patterns emerged highly invasive or localized. The phenotype was dependent on malignancy and previous treatment of the patient from which the xenograft was derived. Physiologically, mice exhibited symptoms more quickly with each subsequent passage, particularly in the localized tumors. Study of these physiologically relevant human xenografts in mice will enable therapeutic screenings in a microenvironment that more closely resembles GBM and may allow development of individualized patient models which may eventually be used for simulating treatment.

scholarly journals Isolation, cultivation and immunofluorescence characterization of lamellar keratinocytes from equine hoof by using explants

2019 ◽  
Vol 39 (4) ◽  
pp. 292-298
Author(s):  
João P.H. Pfeifer ◽  
Vitor H. Santos ◽  
Gustavo Rosa ◽  
Jaqueline B. Souza ◽  
Marcos Jun Watanabe ◽  
...  
Keyword(s):  
Current Knowledge ◽  
Primary Cultures ◽  
Culture Method ◽  
Ki 67 ◽  
Keratinocyte Culture ◽  
Cell Growth And Differentiation ◽  
Equine Hoof ◽  
Horse Health

ABSTRACT: The importance of the hoof to the horse health is clear, and the current knowledge regarding the cellular aspects of hoof keratinocytes is poor. Studies on equine keratinocyte culture are scarce. Developing keratinocyte cultures in vitro is a condition for studies on molecular biology, cell growth and differentiation. Some methods have already been established, such as those for skin keratinocyte culture. However, few methodologies are found for lamellar keratinocytes. The objective of this study was to standardize the equine hoof keratinocyte isolation and cultivation, and then characterize the cell immunophenotype. For this, the primary culture method used was through explants obtained from three regions of the equine hoof (medial dorsal, dorsal, and lateral dorsal). After the cell isolation and cultivation, the cell culture and its explants were stained with anti-pan cytokeratin (pan-CK) (AE1/AE3), vimentin (V9), p63 (4A4), and Ki-67 (MIB-1) antibodies. Cells were grown to third passage, were positive for pan-CK, p63 and Ki-67, and few cells had vimentin positive expression. As for the explants, the epidermal laminae were not stained for vimentin or Ki-67. However, some cells presented positive pan-CK and p63 expression. This study demonstrated the viability of lamellar explants of equine hooves as a form of isolating keratinocytes in primary cultures, as well as characterized the proliferation ability of such keratinocytes in monolayers.

Culture of murine nasal epithelia: model for cystic fibrosis

2006 ◽  
Vol 290 (2) ◽  
pp. L270-L277 ◽  
Author(s):  
B. R. Grubb ◽  
T. D. Rogers ◽  
P. C. Diggs ◽  
R. C. Boucher ◽  
L. E. Ostrowski
Keyword(s):  
Cystic Fibrosis ◽  
Ion Transport ◽  
Ussing Chamber ◽  
Channel Blockers ◽  
Culture Model ◽  
Δf508 Cftr ◽  
Human Airways ◽  
Columnar Cells

The ion transport defects reported for human cystic fibrosis (CF) airways are reproduced in nasal epithelia of the CF mouse. Although this tissue has been studied in vivo using the nasal potential difference technique and as a native tissue mounted in the Ussing chamber, little information is available on cultured murine nasal epithelia. We have developed a polarized cell culture model of primary murine nasal epithelia in which the CF tissue exhibits not only a defect in cAMP-mediated Cl−secretion but also the Na+hyperabsorption and upregulation of the Ca2+-activated Cl−conductance observed in human airways. Both the wild-type and CF cultures were constituted predominantly of undifferentiated cuboidal columnar cells, with most cultures exhibiting a small number of ciliated cells. Although no goblet cells were observed, RT-PCR demonstrated the expression of Muc5ac RNA after ∼22 days in culture. The CF tissue exhibited an adherent layer of mucus similar to the mucus plaques reported in the distal airways of human CF patients. Furthermore, we found that treatment of CF preparations with a Na+channel blocker for 7 days prevented formation of mucus adherent to epithelial surfaces. The cultured murine nasal epithelial preparation should be an excellent model tissue for gene transfer studies and pharmacological studies of Na+channel blockers and mucolytic agents as well as for further characterization of CF ion transport defects. Culture of nasal epithelia from ΔF508 mice will be particularly useful in testing drugs that allow ΔF508 CFTR to traffic to the membrane.

Development and characterization of an endometrial tissue culture model for study of early implantation events

2012 ◽  
Vol 98 (6) ◽  
pp. 1581-1589 ◽  
Author(s):  
Tian-Min Ye ◽  
Ronald T.K. Pang ◽  
Carmen O.N. Leung ◽  
Weimin Liu ◽  
William S.B. Yeung
Keyword(s):  
Tissue Culture ◽  
Endometrial Tissue ◽  
Culture Model ◽  
Tissue Culture Model

scholarly journals Characterization of folate uptake by choroid plexus epithelial cells in a rat primary culture model

2007 ◽  
Vol 104 (6) ◽  
pp. 1494-1503 ◽  
Author(s):  
Jan B. Wollack ◽  
Benedette Makori ◽  
Stuti Ahlawat ◽  
Rajeth Koneru ◽  
Sonia C. Picinich ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Choroid Plexus ◽  
Culture Model ◽  
Choroid Plexus Epithelial

scholarly journals Characterization of a cartilage-like engineered biomass using a self-aggregating suspension culture model: Molecular composition using FT-IRIS

2011 ◽  
Vol 29 (12) ◽  
pp. 1881-1887 ◽  
Author(s):  
Minwook Kim ◽  
Jeffrey J. Kraft ◽  
Andrew C. Volk ◽  
Joan Pugarelli ◽  
Nancy Pleshko ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Molecular Composition ◽  
Culture Model

scholarly journals Physical, chemical, and toxicological characterization of fibrillated forms of cellulose using an in vitro gastrointestinal digestion and co-culture model

2020 ◽  
Vol 9 (3) ◽  
pp. 290-301 ◽  
Author(s):  
Sahar H Pradhan ◽  
Marina R Mulenos ◽  
London R Steele ◽  
Matthew Gibb ◽  
James D Ede ◽  
...  
Keyword(s):  
Food Safety ◽  
Chemical Characterization ◽  
Toxicological Effects ◽  
Simulated Digestion ◽  
Physical Chemical ◽  
Culture Model ◽  
The Usa ◽  
Physical And Chemical

Abstract Fibrillated cellulose is a next-generation material in development for a variety of applications, including use in food and food-contact materials. An alternative testing strategy including simulated digestion was developed to compare the physical, chemical, and biological characteristics of seven different types of fibrillated cellulose, following European Food Safety Authority guidance. Fibrillated forms were compared to a conventional form of cellulose which has been used in food for over 85 years and has Generally Recognized as safe regulatory status in the USA. The physical and chemical characterization of fibrillated celluloses demonstrate that these materials are similar physically and chemically, which composed of the same fundamental molecular structure and exhibit similar morphology, size, size distribution, surface charge, and low levels of impurities. Simulated gastrointestinal and lysosomal digestions demonstrate that these physical and chemical similarities remain following exposure to conditions that mimic the gastrointestinal tract or intracellular lysosomes. A toxicological investigation with an advanced intestinal co-culture model found that exposure to each of the fibrillated and conventional forms of cellulose, in either the pristine or digested form at 0.4% by weight, showed no adverse toxicological effects including cytotoxicity, barrier integrity, oxidative stress, or inflammation. The results demonstrate the physical, chemical, and biological similarities of these materials and provide substantive evidence to support their grouping and ability to read-across data as part of a food safety demonstration.

Use of a Caco-2 cell culture model for the characterization of intestinal absorption of antibiotics

Il Farmaco ◽  
1999 ◽  
Vol 54 (9) ◽  
pp. 594-599 ◽  
Author(s):  
Elia Biganzoli ◽  
Luigi A Cavenaghi ◽  
Roberta Rossi ◽  
Maria C Brunati ◽  
Maria L Nolli
Keyword(s):  
Cell Culture ◽  
Intestinal Absorption ◽  
Cell Culture Model ◽  
Culture Model
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