Purification of a 166mHo solution by successive high-performance liquid chromatography and gravitational chromatography for half-life determination

2014 ◽  
Vol 302 (1) ◽  
pp. 289-295 ◽  
Author(s):  
Florence Guéguen ◽  
Hélène Isnard ◽  
Karsten Kossert ◽  
Carole Bresson ◽  
Céline Caussignac ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Half Life ◽  
High Performance

scholarly journals Decreasing half-life of dieldrin in egg yolk following a single oral administration of aldrin to laying hens

2001 ◽  
Vol 49 (4) ◽  
pp. 465-472
Author(s):  
N. Furusawa
Keyword(s):  
Body Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Single Dose ◽  
Half Life ◽  
High Performance ◽  
Laying Hens ◽  
Egg Yolk ◽  
Single Oral Administration ◽  
Low Levels

Laying hens were treated orally with a single dose of aldrin (AD) 1 mg/kg body weight. Concentrations (μg/g) of AD or its epoxide (= dieldrin, DD) in the yolk of eggs laid for 21 days after AD treatment were determined by normalphase high-performance liquid chromatography. The limits of determination were 0.02 μg/g for AD and 0.03 μg/g for DD, respectively. After AD treatment, although the low levels of AD (mean 0.02–0.03 μg/g) were observed only during a three-day period (from 4th to 6th days), DD (mean 0.15 μg/g) was found already on the 2nd day, indicating that the epoxidation of AD to DD in the hen’s body is rapid. The highest level of DD (mean 0.40 μg/g) was detected on the 6th day, and then DD levels decreased slowly and were detected up to the 21st day. In this decreasing phase, the half-life of DD in the yolk was estimated to be 25.6 days with a 95% confidence interval from 22.7 to 29.4 days.

scholarly journals DISSIPAÇÃO DO HERBICIDA (R,S) HALOXIFOPE EM LATOSSOLO TROPICAL

2004 ◽  
Vol 14 ◽  
Author(s):  
MARCUS BARIFOUSE MATALLO ◽  
LUIZ CARLOS LUCHINI ◽  
FLAVIO MARTINS GARCIA BLANCO ◽  
TEREZINHA BONANHO PERES ◽  
DANIEL GUSTAVO RICHENA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Half Life ◽  
High Performance ◽  
Methanolic Extract ◽  
Tropical Soil ◽  
Soil Samples ◽  
Ultra Sound ◽  
Previously Treated ◽  
Para Determinar

Com o objetivo de estudar o comportamento do herbicida (R,S)-haloxifope em solo tropical realizouse este trabalho para determinar sua dissipação num latossolo roxo eutrófico. Para isso, amostras de solo (em triplicata) foram previamente tratadas com haloxifope metil racêmico e incubadas a 25oC por 0, 6, 10 15, 30 e 60 dias. Após cada período de incubação as amostras foram submetidas a extração em ultra-som, purificadas com diclorometano, evaporadas em rotavapor e ressuspendidas em metanol. O extrato metanólico foi analisado por Cromatografia a Líquido de Alta Eficiência (CLAE). Os dados cinéticos mostraram meia-vida inicial (t0½) de 7,38 dias, o que pode estar relacionado com a atividade microbiana de caráter enantiosseletivo no solo. (R,S)-HALOXYFOP DISSIPATION IN A TROPICAL LATOSOIL Abstract The aim of the work was to study the behavior of the herbicide (R,S)-haloxyfop in a tropical soil. This work was realized to determine its dissipitaion in an eutrophic purple latosoil. Three replicates of soil samples were previously treated with methyl racemic haloxyfop and incubated at 25ºC for 0, 6, 10, 15, 30 and 60 days. After each incubation period the samples were submited to ultra sound extraction, purified with dichoromethane, evaporated and ressuspended in methanol. The methanolic extract was analysed by High Performance Liquid Chromatography (HPLC). The kinetics data revealed initial half life (t01/2) of 7.38 days, which can probably be related to the microbial activity with enantioselective character in soil.

scholarly journals The pharmacokinetics of the injectable dosage form of GK-2 in rabbits

2020 ◽  
pp. 17-21
Author(s):  
R. V. Shevchenko ◽  
A. A. Litvin ◽  
G. B. Kolyvanov ◽  
P. O. Bochkov ◽  
O. G. Gribakina ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Blood Plasma ◽  
Half Life ◽  
High Performance ◽  
Dosage Form ◽  
Single Intravenous Injection ◽  
Injectable Dosage

The pharmacokinetics of the nerve growth factor mimetic GK-2 (dimeric dipeptide mimetic of the 4-th loop NGF, a derivative of fluoro-substituted 5-[2-(5-fluoropyrid-3-yl) -ethyl)]-2,8-dimethyl-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole) in rabbits after a single intravenous injection of an injectable dosage form at dose 24 mg (7,8-8,7 mg/kg). GK-2 concentrations in the blood plasma were determined by high-performance liquid chromatography with massspectrometric detection. GK-2 can be attributed to "short-lived" drugs, since the half-life from the rabbits blood plasma was 0.9±0.1 h.

Liquid chromatographic assay of cerfuroxime in serum.

1978 ◽  
Vol 24 (2) ◽  
pp. 365-367 ◽  
Author(s):  
I Nilsson-Ehle ◽  
P Nilsson-Ehle
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Intravenous Injection ◽  
Half Life ◽  
High Performance ◽  
Reversed Phase ◽  
Serum Concentrations ◽  
Reversed Phase Chromatography ◽  
Other Substances ◽  
Liquid Chromatographic

Abstract We describe a procedure for determining cefuroxime concentrations in serum by using high-performance liquid chromatography. The drug is extracted from serum with dimethylformamide and separated from other substances in the extract by reversed-phase chromatography. The ultraviolet (280-nm) absorption of the effluent was monitored and quantitated based on the height of the cefuroxime peak. Intra-and inter-assay imprecision (cv) was less than 3.1 and 4.3%, respectively. Serum concentrations as low as 1.0 mg/liter could be accurately measured. No interference from various other drugs and antibiotics was found. A biological half-life of 52 min in serum was observed after intravenous injection of the drug into a human volunteer.

Lachrymal Concentration of Norfloxacin after a Single Ocular Instillation in Humans

1994 ◽  
Vol 4 (2) ◽  
pp. 102-104 ◽  
Author(s):  
F. Drago ◽  
E. De Bernardis
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Half Life ◽  
High Performance ◽  
Area Under The Curve ◽  
Elimination Half Life ◽  
Elimination Half

Lachrymal concentrations of the fluoroquinolone norfloxacin were studied in 30 subjects (aged 24-35 years) after a single ocular instillation (0.3% solution, 50 ul) in comparison with ofloxacin instilled at the same dose. Lachrymal levels were measured by high performance liquid chromatography (HPLC). Lachrymal peak levels were comparable 5 min after instillation but norfloxacin appeared to have a longer elimination half-life than ofloxacin. The AUC (area under the curve) for norfloxacin was therefore higher than for ofloxacin (35.9 and 10.7 mg* min/ml, respectively). These data suggest that norfloxacin may have higher corneal retention than ofloxacin, so this antibiotic may be indicated in superficial infections dependent on fluoroquinolone-sensitive bacteria.

scholarly journals Tembotrione Half-Life in Soils with Different Attributes

2019 ◽  
Vol 37 ◽  
Author(s):  
E.M.G. SILVA ◽  
A.T. FARIA ◽  
N.M.E. MARULANDA ◽  
G.A.M. PEREIRA ◽  
D.T. SARAIVA ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Organic Matter ◽  
Liquid Chromatography ◽  
Soil Organic Matter ◽  
Half Life ◽  
High Performance ◽  
Negative Effects ◽  
Soil Attributes ◽  
Essential Condition ◽  
Surface And Groundwater

ABSTRACT: Before recommending an herbicide, it is necessary to know its interactions with the soil attributes that will influence its sorption and half-life in the soil. This knowledge is an essential condition for minimizing any negative effects that may result from its application. However, due to the great diversity of soil and climate in Brazil, especially for products of recent use in the country, such as the herbicide tembotrione, this knowledge is often not available. This may be the main cause of occurrence of intoxication reports in crops carried out in succession to corn sprayed with this herbicide. In order to reduce possible impacts on successor crops and the contamination of surface and groundwater using the high-performance liquid chromatography, the tembotrione half-life was determined in soils with different attributes. The evaluated soils were a Red-Yellow Latosol with and without liming (Viçosa, MG), Red-Yellow Latosol (Rio Paranaíba, MG), Yellow Latosol (Sooretama, ES), and Red-Yellow Latosol with and without liming (Gurupi, TO). The results showed that liming might favor tembotrione degradation in the soil, as well as direct relationships between values of tembotrione half-life and contents of clay and soil organic matter. Tembotrione half-life in samples of Red-Yellow Latosol (without liming) collected in Viçosa and Rio Paranaíba, MG, was higher than 90 days, indicating carryover risks in successive crops to corn sprayed with tembotrione in these regions.

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