Mapping and confirmation of a new sudden death syndrome resistance QTL on linkage group D2 from the soybean genotypes PI 567374 and ‘Ripley’

Many greenhouse screening methods have been tested to evaluate soybean genotypes for reaction to sudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines. These methods generally have proven disappointing in that results are not consistent among methods or do not correlate with field reaction. In the present study, SDS foliar symptom severity among 24 soybean genotypes was compared using three inoculation methods in the greenhouse. The pathogen inoculum was either mixed (seedbed mixing) or layered (seedbed layering) in the soil medium prior to planting seed and then kept on a greenhouse bench or the inoculum was layered in the soil medium and kept in a temperature control water bath. The water bath method was similar to the layering method with the addition of precise temperature control. The water bath method was superior to the other methods in consistency of SDS symptoms among genotypes among trials and in agreement with SDS field reaction. When disease severity data obtained in the greenhouse were regressed with foliar disease data obtained in field trials, R2 values were 0.56, 0.60, and 0.81 for the seedbed mixing, seedbed layering, and water bath methodS, respectively. The improved ability to predict field response using the water bath method likely results from precise control of the temperature in the rhizosphere. The water bath method described herein will increase the efficiency of selection for highly adapted SDS resistant cultivars by reducing the number of genotypes that must be evaluated under field conditions. Accepted for publication 2 August 2005. Published 6 September 2005.

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Reduction of Sudden Death Syndrome Foliar Symptoms and Fusarium virguliforme DNA in Roots Inoculated With Rhizophagus intraradices

Plant Disease ◽

10.1094/pdis-07-19-1500-re ◽

2020 ◽

Vol 104 (5) ◽

pp. 1415-1420 ◽

Cited By ~ 2

Author(s):

Michelle L. Pawlowski ◽

Glen L. Hartman

Keyword(s):

Sudden Death ◽

Mycorrhizal Fungi ◽

Management Practices ◽

Sudden Death Syndrome ◽

Fusarium Virguliforme ◽

Nutrient Analysis ◽

Rhizophagus Intraradices ◽

Progress Curve ◽

Soybean Genotypes ◽

The Impact

There is increasing interest in incorporating arbuscular mycorrhizal fungi (AMF) into agricultural production because of the benefits they provide, including protection against pathogens and pests. Sudden death syndrome (SDS) of soybean is a devastating disease caused by the soilborne pathogen Fusarium virguliforme. Multiple management methods are needed to control SDS. The relationship between F. virguliforme and AMF is not well documented. The goal of this study was to determine whether soybean plants co-inoculated with F. virguliforme and the AMF species Rhizophagus intraradices showed reduced SDS foliar symptom severity and reduced relative F. virguliforme DNA quantities in soybean roots. Six soybean genotypes were inoculated with F. virguliforme alone or with R. intraradices in a greenhouse experiment. Averaged over the six soybean genotypes, area under the disease progress curve values and relative F. virguliforme DNA quantities were 45 and 28% lower (P < 0.05), respectively, in roots co-inoculated with R. intraradices compared with roots of control plants inoculated with F. virguliforme only. Weight of roots co-inoculated with R. intraradices were 58% higher (P < 0.05) compared with roots of plants not inoculated with R. intraradices. Nutrient analysis showed higher boron, phosphorus, potassium, sodium, and sulfur concentrations in root tissues of plants co-inoculated with R. intraradices compared with plants inoculated with F. virguliforme (P < 0.05). Overall, this study showed that R. intraradices reduced SDS severity and relative F. virguliforme DNA quantities while simultaneously increasing growth and nutrient uptake of plants. Further testing of AMF inoculants in the field will indicate whether incorporating them into soybean SDS management practices will reduce the impact of SDS on soybean production.

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Diallel among soybean genotypes with high oil content and resistance to sudden death syndrome

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132014000200004 ◽

2014 ◽

Vol 57 (2) ◽

pp. 178-186 ◽

Cited By ~ 3

Author(s):

Inocencio Junior de Oliveira ◽

Natal Antonio Vello ◽

Paulo de Melo Jorge Vieira ◽

Philip Traldi Wyminersky

Keyword(s):

Sudden Death ◽

Oil Content ◽

Sudden Death Syndrome ◽

Soybean Genotypes

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Effect of Host Resistance to Fusarium virguliforme and Heterodera glycines on Sudden Death Syndrome Disease Severity and Soybean Yield

Plant Health Progress ◽

10.1094/php-rs-13-0100 ◽

2014 ◽

Vol 15 (1) ◽

pp. 1-8 ◽

Cited By ~ 8

Author(s):

L. F. Brzostowski ◽

W. T. Schapaugh ◽

P. A. Rzodkiewicz ◽

T. C. Todd ◽

C. R. Little

Keyword(s):

Sudden Death ◽

Disease Severity ◽

Host Resistance ◽

Soybean Cyst Nematode ◽

Heterodera Glycines ◽

Reproductive Factors ◽

Sudden Death Syndrome ◽

Population Densities ◽

Fusarium Virguliforme ◽

Soybean Genotypes

Fusarium virguliforme, the soilborne fungus that causes sudden death syndrome (SDS), and Heterodera glycines, the soybean cyst nematode (SCN), are economically important pathogens that often occur concomitantly in Kansas soybean fields. To examine F. virguliforme and H. glycines interactions across multiple environments, four soybean genotypes with different levels of resistance to SDS and SCN were planted at three to four locations in northeastern Kansas in 2008 and 2009. Pathogen population densities were quantified at planting (Pi), midseason (Pm), and harvest (Pf). At harvest, SDS AUDPC, F. virguliforme root population densities, H. glycines reproductive factors (RF), and yield were determined. The performance of resistant (R) genotypes varied with environment and disease pressure, but SDS-R genotypes were associated with 36% greater yields than SDS-susceptible (S) genotypes in high SDS environments. Even moderate levels of SCN resistance reduced SDS disease severity in SDS-S genotypes. Negative correlations (P ≤ 0.05) were observed between yield and AUDPC, and yield and F. virguliforme root population densities. A regression model that combined both of these covariates explained 57% of the yield variation. Disease severity was positively correlated with H. glycines Pi, but negatively correlated with RF. The data emphasize the importance of combining SDS and SCN host resistance in fields with a history of both diseases. Accepted for publication 6 November 2013. Published 27 January 2014.

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Inoculation methods under greenhouse conditions for evaluating soybean resistance to sudden death syndrome

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2008001100005 ◽

2008 ◽

Vol 43 (11) ◽

pp. 1475-1482 ◽

Cited By ~ 6

Author(s):

Austeclinio Lopes de Farias Neto ◽

Michael Schmidt ◽

Glen Lee Hartman ◽

Li Shuxian ◽

Brian Willians Diers

Keyword(s):

Sudden Death ◽

Field Resistance ◽

Sudden Death Syndrome ◽

Screening Methods ◽

Inoculation Methods ◽

Foliar Symptom ◽

Soybean Genotypes ◽

Soybean Resistance ◽

Greenhouse Screening ◽

Sorghum Bicolor L

The objectives of this work were to evaluate two greenhouse screening methods for sudden death syndrome (SDS) and to determine which one is best correlated with field resistance of soybean genotypes. The evaluations were done with three sets of genotypes that were classified as partially resistant, intermediate, and susceptible to SDS based on previous field evaluations. These three sets were independently evaluated for greenhouse SDS reactions using cone and tray inoculation methods. Plants were infected using grains of white sorghum [Sorghum bicolor (L.) Moench] infested with Fusarium solani f. sp. glycines. Foliar symptom severity was rated 21 days after emergence. The cone and field SDS ratings were significantly correlated and ranged from 0.69 for set 1 to 0.51 for set 3. Correlations of SDS ratings of genotypes between field and greenhouse tray ratings were significant for set 1 and not significant for set 2. The cone method showed the highest correlation with field results and is recommended to screen soybean genotypes for SDS resistance.

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Optimizing Conditions of a Cell-Free Toxic Filtrate Stem Cutting Assay to Evaluate Soybean Genotype Responses toFusariumSpecies that Cause Sudden Death Syndrome

Plant Disease ◽

10.1094/pdis-08-14-0791-re ◽

2015 ◽

Vol 99 (4) ◽

pp. 502-507 ◽

Cited By ~ 6

Author(s):

Y. Xiang ◽

M. M. Scandiani ◽

T. K. Herman ◽

G. L. Hartman

Keyword(s):

Sudden Death ◽

Plant Root ◽

Sudden Death Syndrome ◽

Stem Cutting ◽

Stem Cuttings ◽

Soybean Genotype ◽

Culture Filtrates ◽

Whole Plant ◽

Soybean Genotypes ◽

Toxic Culture

Cell-free toxic culture filtrates from Fusarium virguliforme, the causal fungus of soybean sudden death syndrome (SDS), cause foliar symptoms on soybean stem cuttings similar to those obtained from root inoculations in whole plants and those observed in production fields. The objectives of this study were to (i) optimize the production conditions for F. virguliforme cell-free toxic culture filtrates and the incubation conditions of the stem cutting assay used to test the toxicity of the cell-free toxic culture filtrates, and (ii) use the optimized assay and a whole plant root inoculation assay to compare four SDS-causing isolates on a panel of selected soybean genotypes. Area under the disease progress curve (AUDPC) values were highest (P = 0.05) when cuttings were immersed in culture filtrate of fungus grown in soybean dextrose broth, in filtrate produced from the fungus grown for 18 or 22 days, and when stem cuttings were incubated at 30°C. AUDPC values and shoot dry weights from the whole plant root inoculations and the AUDPC values from the stem cutting assay differed (P < 0.05) among nine soybean genotypes tested with F. virguliforme and F. tucumaniae isolates, and the AUDPC values from the two assays were positively correlated (r = 0.44 at P < 0.0001).

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Diet, total plasma homocysteine concentrations and mortality rates in broiler chickens

Canadian Journal of Animal Science ◽

10.4141/a02-029 ◽

2003 ◽

Vol 83 (3) ◽

pp. 601-604 ◽

Cited By ~ 1

Author(s):

S. E. Samuels

Keyword(s):

Cardiovascular Disease ◽

Sudden Death ◽

Broiler Chickens ◽

Independent Risk Factor ◽

Total Mortality ◽

Mortality Rates ◽

Plasma Homocysteine ◽

Sudden Death Syndrome ◽

Total Plasma ◽

Elevated Plasma

The aim of this study was to determine if total plasma homocysteine (HCY) concentrations and mortality rates due to ascites syndrome and (AS) sudden death syndrome (SDS) in broiler chickens could be lowered by diet. Elevated plasma HCY is an independent risk factor for cardiovascular disease in humans. A total of 828 day-old male broiler chickens (Arbor Acre) were fed, for 6 wk, either a basal practical diet or one supplemented with excess vitamins B6 and B12, folic acid and betaine to stimulate the degradation of HCY. The supplemented diet decreased plasma HCY by 17% (P < 0.05; n = 16 per diet). Total mortality due to AS and SDS was 18% lower in the supplemented diet but this difference was not statistically significant. Key words: Homocysteine, folate, chickens, cardiovascular disease, ascites, sudden death syndrome

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The Genome Sequence of the Fungal Pathogen Fusarium virguliforme That Causes Sudden Death Syndrome in Soybean

PLoS ONE ◽

10.1371/journal.pone.0081832 ◽

2014 ◽

Vol 9 (1) ◽

pp. e81832 ◽

Cited By ~ 35

Author(s):

Subodh K. Srivastava ◽

Xiaoqiu Huang ◽

Hargeet K. Brar ◽

Ahmad M. Fakhoury ◽

Burton H. Bluhm ◽

...

Keyword(s):

Sudden Death ◽

Genome Sequence ◽

Fungal Pathogen ◽

Sudden Death Syndrome ◽

Fusarium Virguliforme

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Role of cardiac hypoxia in the pathogenesis of sudden death syndrome in broiler chickens – A metabolic and molecular study

Acta Veterinaria Hungarica ◽

10.1556/004.2021.00004 ◽

2021 ◽

Author(s):

Pegah Safaei ◽

Gholamhossein Khadjeh ◽

Mohammad Reza Tabandeh ◽

Keramat Asasi

Keyword(s):

Sudden Death ◽

Cardiac Muscle ◽

Internal Control ◽

Broiler Chickens ◽

Sudden Death Syndrome ◽

Pyruvate Dehydrogenase Kinase ◽

Cardiac Insufficiency ◽

Monocarboxylate Transporter ◽

Pcr Analysis ◽

Glucose Transporter 1

AbstractSudden death syndrome (SDS) is an economically important disorder in broiler chickens with unknown aetiology. The aim of the present study was to evaluate the metabolic and molecular alterations related to hypoxia in the myocardium of broiler chickens with SDS. Samples from the cardiac muscle of internal control broiler chickens (ICs) (n = 36) and chickens having died of SDS (n = 36) were obtained during the rearing period. The activities of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) and the concentration of lactate were measured in the cardiac tissue using available commercial kits. The expression of hypoxia-inducing factor 1α (HIF1α), glucose transporter 1 (GLUT1), pyruvate dehydrogenase kinase 4 (PDHK4) and monocarboxylate transporter 4 (MCT4) genes was determined in the myocardium by real-time PCR analysis. The results showed the elevation of lactate level and activities of LDH and CPK in the cardiac muscle of SDS-affected chickens compared with the IC birds (P < 0.05). The cardiac muscle expression of HIF1α, MCT4 and GLUT1 genes was increased, while the PDHK4 mRNA level was decreased in the SDS-affected group compared to those in the IC chickens (P < 0.05). Our results showed that metabolic remodelling associated with hypoxia in the cardiac tissues may have an important role in the pathogenesis of cardiac insufficiency and SDS in broiler chickens.

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