To Study the Effect of Auxin Concentrations (IBA and IAA) on Growth Performance of Stem Cutting of Hardwickia binata (Roxb.)

The present studies on “Effect of Plant Growth Regulators on Growth Performance of Stem Cutting of Hardwickia binata Roxb” was carried out in the mist chamber of Institute of Forest Biodiversity, Dulapally, Kompally, Hyderabad, during January- April, 2021. The cuttings of Hardwickia binata Roxb were prepared from selected superior phenotypes from the surrounding environments of Kompally, Hyderabad. Hardwickia binata Roxb. is difficult to root and need auxin treatment and controlled environment to successful survival and rooting. The cuttings were treated with 0, 200, 500,1000, 1500, 2000 mg/l concentrations of IBA and IAA and planted in poly bags under mist chamber. The experiment was conducted in a completely randomized design. Analysis of variance was worked out to assess the variation in Hardwickia binata Roxb. for eight characters. Treatment with IBA at 2000mg was found to be the best treatment as it achieved maximum in survival percentage (52.64%), shoot length (31.92cm), rooting percentage (81.69%), number of roots per cutting (20.44) and root biomass (6.32g) followed by treatment with IAA at 2000mg/l (T10) achieved maximum. The performance increased with an increase in concentrations of both IBA and IAA. Among the two auxins treatments, IBA was found to be most effective, showed significantly higher values compared to IAA. It is possible to successfully multiply the Hardwickia binata Roxb. cuttings by treating with 2000 mg/l of IBA under controlled phyto-environmental condition. The study evolved an easy and efficient protocol for vegetative propagation of HardwickiabinataRoxb.via stem cuttings to establish clonal forestry.

EFFECT OF STEM DIAMETER, MEDIA TYPE AND LIGHT INTENSITY ON THE GROWTH OF MEDICINAL PLANT OF SCREW TREE/KAYU ULES (Helicteres isora Linn.)

Screw tree (Helicteres isora) is a shrub species (Fam: Malvaceae) with 1.5–4 m in height and with multi-main trunks up to 9–12. In Indonesia, kayu ules has been used as a traditional medicine, known as jamu, and has been commercialized. In order to cultivate this species, vegetative propagation is likely promising compared to the generative propagation since seed viability is low and cannot be stored for longterm. Nevertheless, there is a lack of information on which diameter classes, media types and light intensities would give the best result for vegetative propagation. This research aimed to obtain information on which diameter classes and media that were suitable for kayu ules stem cutting propagation and to what degree the light intensity would be best for the growth of the seedlings of kayu ules. Three diameter classes and four media types were employed in a complete random design under the greenhouse environment. Three months old good quality vegetatively-propagated seedlings were transplanted under two light intensities (under transparent canopy and shading net). The result showed that considering the factor of diameter classes and media in kayu ules stem cutting propagation, it had improved the survival of the stem cutting. Diameter class I was best grown in media type 3 with 46.67 ± 12.2 % of survival rate, diameter class II was best grown in media type 2 with 38.67 ± 11.6 % of survival rate, whereas diameter class III was best grown in media type 4 with 52 ± 6.9 % of survival rate. The transplanted seedlings also grew significantly better under transparent canopy with higher light intensity. The distinctive features of screw tree under shading net were wider leaf area, higher specific leaf area and relative water content but it was lower in above ground dry biomass compared to the kayu ules under transparent canopy.

Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

Hoya imperialis (H. imperialis) and H. coronaria (Apocynaceae) are known to have ornamental value due to their beautiful flowers; however, the feasibility of propagating these plants have not been reported despite the wild populations in Brunei Darussalam being highly threatened due to habitat loss and overcollection. Thus, the present study aimed to conduct a preliminary study of the feasibility of two alternative propagation methods, stem cutting and micropropagation, as a potential approach for their ex situ conservation. Hoya stem cuttings were treated with either indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) (0–2000 mg/L), and then propagated onto a mixture of peat moss and perlite. For micropropagation, Hoya leaf explants were cultured onto Murashige and Skoog (MS) agar media that were supplemented with IBA and/or kinetin (KN) (0–10.0 mg/L). This present study shows that both Hoya species were successfully propagated by stem cutting even without hormone treatment. However, interestingly, in H. imperialis, when compared with control, the mean number of new leaves (6.3 ± 1.0) and the mean relative growth rate (RGR) based on stem diameter (0.004 ± 0.0007 cm cm−1 day−1) significantly increased when treated with 500 mg/L NAA and 2000 mg/L IBA, respectively. Meanwhile, in H. coronaria, significantly higher mean number of roots was achieved by treating with 1000 mg/L NAA (16.6 ± 1.4) or 2000 mg/L IBA (17.5 ± 2.7) compared with control. For micropropagation, callus induction was not promising and could only be observed at specific concentrations of both IBA and KN, with H. imperialis appearing to be more responsive towards these hormones in comparison to H. coronaria. The present study showed that stem cutting appeared more feasible in propagating both Hoya species.

Study on Stem Cutting Propagation of Gynostemma Pentaphyllum (Thunb.) Makino in Hoa Binh Province

Gynostemma pentaphyllum (Thunb.) Makino is a medicinal plant distributed mostly in the mountainous areas of Vietnam. G. pentaphyllum contains more than 189 types of saponin which can be used for the treatment of various diseases. Currently, due to over-exploitation, this plant species is under threaten. Our research aimed to study a suitable method for plant propagation by stem cutting techniques to promote the growth and development of G. pentaphyllum. Six experiments were conducted to (i) evaluate the established seedling rates of different G. pentaphyllum varieties, and choose the best variety to (ii) evaluate the established seedling rate influenced by different factors, viz. stem cutting position, cutting season, growing in the selected substrates, stem cutting length, number of buds per stem, and application of α-NAA. The results showed that among five varieties, G2 which was collected in Mai Chau district disclosed the highest ability to shooting, the highest number of roots per stem cutting, the shortest nursery days, the highest shooting rate, and the highestestablished seedling rate. We also determined that using middle-aged plants for stem cuttings, at 15-20cm in length, with 2 nodes and grown in Spring and Winter cropping seasons, using growing substrates with a mixture of 50% of black sand and 50% of soil, and plus α-NAA 50ppm in 3-5min produced the highest explant rate. The established seedlingsat the time of explant were 15-20cm in height, containing 4-5 leaves and at least 9-10 roots per cutting, and were 30-32 days.

​Regeneration of Stem Cuttings of Pomegranate cv. Bhagwa as Influenced by PGR’s and Planting Time

Background: Pomegranate is being cultivated in the tropical and sub-tropical parts of the world for its delicious fruits. It can be propagated from seeds as well as from softwood, semi-hardwood and hardwood cuttings. The application of auxins encourages rooting in stem cutting owing to their ability to active cambium regeneration, cell division and cell multiplication. To meet the demand of pomegranate in the current situation and owing to its importance, the present study was undertaken. Methods: For regeneration of stem cuttings of pomegranate cv. Bhagwa as influenced by PGR‘s and planting time a study was conducted at the Department of Horticulture, Khalsa College, Amritsar during 2019-2020. The trial was undertaken with ten treatments comprising of IBA, PHB and NAA (500, 750 and 1000 ppm) each by quick dip method along with control planted in the first week of August and January. Result: The results of the study revealed that IBA 1000 ppm (T3) proved to be the best in terms of minimum days to first sprouting (11.06), maximum sprouting (91.10%), rooting (83.31%), number of roots per cutting (60.03), root length (11.55 cm), fresh weight of roots (1.08 g) and dry weight of roots (0.55 g). Among planting time the cuttings planted on first week of January proved to be effective in terms of sprouting (87.83%), survival (84.70%), rooting (78.81%) and number of roots (52.06) respectively.

Early Root Development of Eucalyptus pellita F. Muell. Seedlings from Seed and Stem Cutting Propagation Methods at Nursery Stage

Macropropagation using cutting for larger multiplying seedlings is cheaper and efficient instead of clonal seeds for uniform plant material seedling production. However, information on root growth of Eucalyptus pellita at early development from seed and stem cutting of E. pellita seedlings is still lacking. With such information, it is useful for forest plantation company management in enhancing the understanding of strategies to optimize yield production with the appropriate agronomic or silvicultural approach in the field of planting. Therefore, the objectives of this study were to compare the root development of two different types of propagation seedlings of E. pellita and to study the effect of various nitrogen concentration levels on two different types of propagation of E. pellita seedlings. The study was conducted using E. pellita seedlings from two different types of propagation, namely, seed and stem cuttings, along with three different nitrogen concentrations (0, 50, and 200 kg N ha−1). Shoot biomass, root intensity (RI), total root intensity (TRI), root biomass, root length density (RLD), and specific root length (SRL) were recorded. Dried shoot biomass, RLD, and SRL of E. pellita seedlings using stem cutting were significantly higher ( P < 0.05 ) compared to seed, whereas there were no significant differences ( P > 0.05 ) for root biomass, TRI, and RI between the propagation types of E. pellita seedlings. In conclusion, E. pellita seedlings from stem cutting were greater in terms of root distribution compared to propagation by seeds at the nursery stage, and 50 kg N ha−1 was the optimal nitrogen concentration level from the considered levels to be applied to the E. pellita seedlings.

Agronomic Character of Ratoon Rice: Stem Cutting Sizes and Seprint Liquid Organic Fertilizer

This study aimed to identify the optimum rice stem cutting size combined with the application of Seprint liquid organic fertilizer (SLOF) to promote the growth and yield of ratoon rice. This research was conducted in a greenhouse at the Faculty of Agriculture, University of Muhammadiyah Sumatera Utara, North Sumatra Province, Indonesia, from January to August 2018. The experiment was set up as a completely randomized design factorial with four replications. The first factor consisted of the cutting sizes of the rice stem (R1 = 5 cm, R2 = 10 cm, and R3 = 15 cm) and the second factor involved the SLOF dosage (S1 = 5 mL L −1 , S2 = 10 mL L −1 , and S3 = 15 mL L −1 ). The parameters were analyzed using analysis of variance (ANOVA), and the means were determined by Duncan’s multiple range test (DMRT). The results showed that the R2 treatment significantly increased the ratoon rice height at 10 to 30 days after cutting (DAC), the number of tillers at 40 to 60 DAC, the number of productive tillers, and the yield per hectare. The S2 and S3 applications at significantly increased the yield per hill and ratoon rice height at 20 DAC. The R2S1 and R2S2 combination treatments significantly increased the number of filled grains per panicle and the ratoon rice height at 10 DAC. Cutting the stems at 10 cm combined with various dosages of SLOF resulted in greater growth and yield of ratoon rice for achieving an adequate food supply.