Age-Related ECoG Dynamics of and Convulsive Activity of Wistar Rats in a Cortical Model of Focal Epilepsy

2018 ◽  
Vol 48 (8) ◽  
pp. 1006-1013 ◽  
Author(s):  
D. S. Kalinina ◽  
D. N. Lenkov ◽  
I. A. Zhuravin ◽  
A. B. Vol’nova
Keyword(s):  
Wistar Rats ◽  
Focal Epilepsy ◽  
Cortical Model ◽  
Convulsive Activity ◽  
Age Related

Renal Cortical Slices: An In Vitro Model for Kidney Metabolism and Toxicity

1992 ◽  
Vol 20 (1) ◽  
pp. 71-76
Author(s):  
Andrea Trevisan ◽  
Stefano Maso ◽  
Paola Meneghetti
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
Organic Anion ◽  
Cortical Slice ◽  
Lactate Dehydrogenase Release ◽  
Age Related ◽  
Renal Cortical Slice ◽  
Male Wistar Rats ◽  
Vitro Model

The in vitro renal cortical slice model was used to study: 1) the effects on the kidney of some haloalkanes and haloalkenes using 3-month-old male Wistar rats; 2) influence of age and sex on renal cortical slice indices in non-treated rats; and 3) effects of 1,2-dichloropropane on the slices after pretreatment of 3-month-old male Wistar rats with DL-butathionine-[S,R]-sulphoximine. The most nephrotoxic chemical used was 1,3-dichloropropene, which caused a total depletion in the levels of reduced glutathione, a high peroxidation of lipid (about three thousand-fold with respect to control), a significant release of tubular enzymes into the medium, and loss of organic anion ( p-aminohippurate) accumulation. All the chemicals affected the cytosol more than the brush border. The most remarkable age-related differences in the untreated slices were the progressive decrease of reduced glutathione (p<0.05 from three months of age), and an increase in lactate dehydrogenase release into the medium (p<0.05 from six months of age). By contrast, sex differences were slight. The ‘treatment with 1,2-dichloropropane of slices prepared from rats pretreated with DL-butathionine-[S,R]-sulphoximine significantly increased the depletion of glutathione content (p<0.05) and malondialdehyde release in the medium (p<0.001) caused by the solvent alone.

Age-related variations in plasma and liver lipids of Yoshida rats: a comparison with Wistar rats

1995 ◽  
Vol 111 (2) ◽  
pp. 319-327 ◽  
Author(s):  
R. Masella ◽  
E. Pignatelli ◽  
T. Marinelli ◽  
D. Modesti ◽  
R. Verna ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Liver Lipids ◽  
Age Related ◽  
Plasma And Liver Lipids

scholarly journals Age-Induced Loss of Mossy Fibre Synapses on CA3 Thorns in the CA3 Stratum Lucidum

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Bunmi Ojo ◽  
Heather Davies ◽  
Payam Rezaie ◽  
Paul Gabbott ◽  
Francis Colyer ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Synaptic Connection ◽  
Mossy Fibre ◽  
Synaptic Density ◽  
Cellular Processes ◽  
Unbiased Stereology ◽  
Microvascular Changes ◽  
Age Related ◽  
Stratum Lucidum ◽  
Serial Electron Microscopy

Advanced ageing is associated with hippocampal deterioration and mild cognitive decline. The hippocampal subregion CA3 stratum lucidum (CA3-SL) receives neuronal inputs from the giant mossy fibre boutons of the dentate gyrus, but relatively little is known about the integrity of this synaptic connection with ageing. Using serial electron microscopy and unbiased stereology, we examined age-related changes in mossy fibre synapses on CA3 thorny excrescences within the CA3-SL of young adults (4-month-old), middle-aged (12-month-old), and old-aged (28-month-old) Wistar rats. Our data show that while there is an increase in CA3 volume with ageing, there is a significant (40–45%) reduction in synaptic density within the CA3-SL of 12- and 28-month-old animals compared with 4-month-old animals. We also present preliminary data showing that the CA3 neuropil in advanced ageing was conspicuously full of lipofuscin and phagolysosome positive, activated microglial cellular processes, and altered perivascular pathology. These data suggest that synaptic density in the CA3-SL is significantly impaired in ageing, accompanied by underlying prominent ultrastructural glial and microvascular changes.

scholarly journals Quantitative MRI susceptibility mapping reveals cortical signatures of changes in iron, calcium and zinc in malformations of cortical development in children with drug-resistant epilepsy

2020 ◽  
Author(s):  
Sara Lorio ◽  
Jan Sedlacik ◽  
Po-wah So ◽  
Harold G Parks ◽  
Roxane Gunny ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Focal Epilepsy ◽  
Red Nucleus ◽  
Cortical Development ◽  
Susceptibility Mapping ◽  
Transverse Relaxation Rate ◽  
Drug Resistant ◽  
Malformations Of Cortical Development ◽  
X Ray ◽  
Age Related

Objective Malformations of cortical development (MCD), including focal cortical dysplasia (FCD), are the most common cause of drug-resistant focal epilepsy in children. Histopathological lesion characterisation demonstrates abnormal cell types and lamination, alterations in myelin (typically co-localised with iron), and sometimes calcification. Quantitative susceptibility mapping (QSM) is an emerging MRI technique that measures tissue magnetic susceptibility (χ) reflecting its mineral composition. We used QSM to investigate abnormal tissue composition in a group of children with focal epilepsy with comparison to effective transverse relaxation rate (R2*) and Synchrotron radiation X-ray fluorescence (SRXRF) elemental maps. Our primary hypothesis was that reductions in χ would be found in FCD lesions, resulting from alterations in their iron and calcium content. We also evaluated deep grey matter nuclei for changes in χ with age. Methods QSM (χ) and R2* maps were calculated for 40 paediatric patients with suspected FCD (18 histologically confirmed) and 17 age-matched controls. Patients sub-groups were defined based on concordant electro-clinical or histopathology data. Quantitative investigation of QSM and R2* were performed within lesions, using a surface-based approach with comparison to homologous regions, and globally within deep brain regions using a voxel-based approach with regional values modelled with age and epilepsy as covariates. Synchrotron radiation X-ray fluorescence (SRXRF) was performed on brain tissue resected from 4 patients to map changes in iron, calcium and zinc and relate them to MRI parameters. Results Compared to fluid‐attenuated inversion recovery (FLAIR) or T1‐weighted imaging, QSM improved lesion conspicuity in 5% of patients. In patients with well-localised and confirmed FCDIIb lesions, quantitative profiling demonstrated decreased χ, but not R2*, across cortical depth with respect to the homologous regions. Contra-lateral homologous regions additionally exhibited increased χ at 2-3mm cortical depth that was absent in lesions. The iron decrease measured by the SRXRF in FCDIIb lesions was in agreement with myelin reduction observed by Luxol Fast Blue histochemical staining. SRXRF analysis in two FCDIIb tissue samples showed increased zinc and calcium, and decreased iron in the brain region exhibiting low χ and high R2*. QSM revealed expected age-related changes in the striatum nuclei, substantia nigra, sub-thalamic and red nucleus, but these changes were not altered in epilepsy. Conclusion QSM non-invasively revealed cortical/sub-cortical tissue alterations in MCD lesions and in particular that χ changes in FCDIIb lesions were consistent with reduced iron, co-localised with low myelin and increased calcium and zinc content. Theses findings suggests that the measurements of cortical χ measurements could be used to detect and delineate epilepsy lesions.

scholarly journals Age-related Individual Behavioural Characteristics of Adult Wistar Rats

2021 ◽  
Author(s):  
Sergey Sudakov ◽  
Elena Alekseeva ◽  
Galina Nazarova ◽  
Valentina Bashkatova
Keyword(s):  
Motor Activity ◽  
Wistar Rats ◽  
Individual Characteristics ◽  
Female Rats ◽  
Related Individual ◽  
Male And Female Rats ◽  
Age Related ◽  
Behavioural Characteristics ◽  
Plus Maze ◽  
Age Related Changes

The aim of this work was to study age-related changes in the behaviour of adult Wistar rats using the open field (OF) and elevated plus maze (EPM) tests. Behavioural changes related to motor activity and anxiety were of particular interest. Results showed that as male and female rats progressed from 2 to 5 months of age there was a decrease in the level of motor and exploratory activities, and an increase in the level of anxiety. Age-related changes were dependent upon initial individual characteristics of behaviour. For example, animals that demonstrated high motor activity at 2 months become significantly less active by 5 months, and animals that showed a low level of anxiety at 2 months become more anxious by 5 months. Low-activity and high-anxiety rats did not show any significant age-related changes in OF and EPM tests from 2 to 5 months of age, except for a decrease in the number of rearings in EPM. Significant individual differences in the behaviour of rats in OF and EPM tests observed at 2 months were not apparent by 5 months.

scholarly journals Wistar rats: a forgotten model of age-related hearing loss

2014 ◽  
Vol 6 ◽  
Author(s):  
Juan C. Alvarado ◽  
Verónica Fuentes-Santamaría ◽  
María C. Gabaldón-Ull ◽  
José L. Blanco ◽  
José M. Juiz
Keyword(s):  
Hearing Loss ◽  
Wistar Rats ◽  
Age Related ◽  
Age Related Hearing Loss
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