Effective Gene Delivery to Mesenchymal Stem Cells Based on the Reverse Transfection and Three-Dimensional Cell Culture System

2011 ◽  
Vol 28 (7) ◽  
pp. 1577-1590 ◽  
Author(s):  
Cai-Xia He ◽  
Ni Li ◽  
Yu-Lan Hu ◽  
Xiu-Mei Zhu ◽  
Hai-Jie Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Reverse Transfection ◽  
Dimensional Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

scholarly journals Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems

2018 ◽  
Vol 6 (3) ◽  
pp. 501-510 ◽  
Author(s):  
Emanuele Mauri ◽  
Alessandro Sacchetti ◽  
Nunzio Vicario ◽  
Luca Peruzzotti-Jametti ◽  
Filippo Rossi ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Nervous System Diseases ◽  
Central Nervous System Diseases ◽  
Hybrid Hydrogels ◽  
Cell Culture Systems ◽  
Dimensional Cell

The use of neural stem cells (NSCs) in cell therapy has become a powerful tool used for the treatment of central nervous system diseases and the design of a three-dimensional cell culture system to improve NSCs viability is a challenge.

Study on Pipetting Motion Optimization of Automatic Spheroid Culture System for Spheroid Formation

2021 ◽  
Vol 33 (1) ◽  
pp. 78-87
Author(s):  
Takeshi Shimoto ◽  
Chihiro Teshima ◽  
Toshiki Watanabe ◽  
Xiu-Ying Zhang ◽  
Atsushi Ishikawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture System ◽  
Three Dimensional ◽  
Spheroid Formation ◽  
Spheroid Culture ◽  
Motion Optimization ◽  
Passage Number ◽  
Dimensional Cell ◽  
Number Of Cells

This research group has established a technology for producing a three-dimensional cell constructed using only the cell itself. This technology uses a property in which the spheroids fuse with each other. We developed a system that automates the spheroid production process to obtain reproducible spheroids and suppress variation factors that occur from human operation. However, it has become clear that the dispersion occurs in the diameter depending on the number of cells of the spheroid even if the cells are handled in the same manner. The purpose of this research is to examine an appropriate pipetting motion in accordance with the number of cells of the spheroid to be produced. Rabbit mesenchymal stem cells (rMSCs) are used as the objects. The number of cells was set to 2×104, 3×104, and 4×104 cells/well, and the passage number as 7. The appearance of spheroids cultured using the motion programmed in accordance with each number of cells was observed every 24 hours for 5 days after seeding. The results of the analysis indicate that the optimum motion in each number of cells has been successfully specified, and reproducible spheroids have been successfully produced.

A novel in vitro permeability assay using three-dimensional cell culture system

2015 ◽  
Vol 205 ◽  
pp. 93-100 ◽  
Author(s):  
Jong Bong Lee ◽  
Sung Hwa Son ◽  
Min Chul Park ◽  
Tae Hwan Kim ◽  
Min Gi Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Permeability Assay ◽  
Dimensional Cell

Retinoic acid induces mouse bone marrow-derived CD15+, Oct4+and CXCR4+stem cells into male germ-like cells in a two-dimensional cell culture system

2014 ◽  
Vol 38 (6) ◽  
pp. 782-789 ◽  
Author(s):  
Iraj Ragerdi Kashani ◽  
Amir Hassan Zarnani ◽  
Masoud Soleimani ◽  
Mir Abbas Abdolvahabi ◽  
Karim Nayernia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Retinoic Acid ◽  
Culture System ◽  
Mouse Bone Marrow ◽  
Two Dimensional ◽  
Cell Culture System ◽  
Dimensional Cell
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