Spray-Dried Chitosan Microparticles for Cellular Delivery of an Antigenic Protein: Physico-chemical Properties and Cellular Uptake by Dendritic Cells and Macrophages

2013 ◽  
Vol 30 (6) ◽  
pp. 1677-1697 ◽  
Author(s):  
Chirasak Kusonwiriyawong ◽  
Vimolmas Lipipun ◽  
Nontima Vardhanabhuti ◽  
Qiang Zhang ◽  
Garnpimol C. Ritthidej
Keyword(s):  
Dendritic Cells ◽  
Cellular Uptake ◽  
Chemical Properties ◽  
Cellular Delivery ◽  
Antigenic Protein ◽  
Chitosan Microparticles ◽  
Physico Chemical ◽  
Spray Dried

scholarly journals Size and surface modification of silica nanoparticles affect the severity of lung toxicity by modulating endosomal ROS generation in macrophages

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Masahide Inoue ◽  
Koji Sakamoto ◽  
Atsushi Suzuki ◽  
Shinya Nakai ◽  
Akira Ando ◽  
...  
Keyword(s):  
Immune Response ◽  
Surface Modification ◽  
Lung Injury ◽  
Cellular Uptake ◽  
Intratracheal Instillation ◽  
Chemical Properties ◽  
Raw264.7 Cells ◽  
Neutrophilic Infiltration ◽  
Silica Nanomaterials ◽  
Physico Chemical

Abstract Background As the application of silica nanomaterials continues to expand, increasing chances of its exposure to the human body and potential harm are anticipated. Although the toxicity of silica nanomaterials is assumed to be affected by their physio-chemical properties, including size and surface functionalization, its molecular mechanisms remain unclear. We hypothesized that analysis of intracellular localization of the particles and subsequent intracellular signaling could reveal a novel determinant of inflammatory response against silica particles with different physico-chemical properties. Results We employed a murine intratracheal instillation model of amorphous silica nanoparticles (NPs) exposure to compare their in vivo toxicities in the respiratory system. Pristine silica-NPs of 50 nm diameters (50 nm-plain) induced airway-centered lung injury with marked neutrophilic infiltration. By contrast, instillation of pristine silica particles of a larger diameter (3 μm; 3 μm-plain) significantly reduced the severity of lung injury and neutrophilic infiltration, possibly through attenuated induction of neutrophil chemotactic chemokines including MIP2. Ex vivo analysis of alveolar macrophages as well as in vitro assessment using RAW264.7 cells revealed a remarkably lower cellular uptake of 3 μm-plain particles compared with 50 nm-plain, which is assumed to be the underlying mechanism of attenuated immune response. The severity of lung injury and neutrophilic infiltration was also significantly reduced after intratracheal instillation of silica NPs with an amine surface modification (50 nm-NH2) when compared with 50 nm-plain. Despite unchanged efficacy in cellular uptake, treatment with 50 nm-NH2 induced a significantly attenuated immune response in RAW264.7 cells. Assessment of intracellular redox signaling revealed increased reactive oxygen species (ROS) in endosomal compartments of RAW264.7 cells treated with 50 nm-plain when compared with vehicle-treated control. In contrast, augmentation of endosomal ROS signals in cells treated with 50 nm-NH2 was significantly lower. Moreover, selective inhibition of NADPH oxidase 2 (NOX2) was sufficient to inhibit endosomal ROS bursts and induction of chemokine expressions in cells treated with silica NPs, suggesting the central role of endosomal ROS generated by NOX2 in the regulation of the inflammatory response in macrophages that endocytosed silica NPs. Conclusions Our murine model suggested that the pulmonary toxicity of silica NPs depended on their physico-chemical properties through distinct mechanisms. Cellular uptake of larger particles by macrophages decreased, while surface amine modification modulated endosomal ROS signaling via NOX2, both of which are assumed to be involved in mitigating immune response in macrophages and resulting lung injury.

Effect of operating parameters on the surface and physico-chemical properties of spray-dried camel milk powders

2018 ◽  
Vol 112 ◽  
pp. 137-149 ◽  
Author(s):  
Haileeyesus Habtegebriel ◽  
Dintwa Edward ◽  
Michael Wawire ◽  
Daniel Sila ◽  
Eyassu Seifu
Keyword(s):  
Chemical Properties ◽  
Camel Milk ◽  
Operating Parameters ◽  
Physico Chemical ◽  
Spray Dried

scholarly journals Physico-Chemical Properties, Aerosolization and Dissolution of Co-Spray Dried Azithromycin Particles with L-Leucine for Inhalation

2018 ◽  
Vol 35 (2) ◽  
Author(s):  
Sharad Mangal ◽  
Haichen Nie ◽  
Rongkun Xu ◽  
Rui Guo ◽  
Alex Cavallaro ◽  
...  
Keyword(s):  
Chemical Properties ◽  
Physico Chemical ◽  
Spray Dried

scholarly journals Effect of Physico-Chemical Properties of Nanoparticles on Their Intracellular Uptake

2020 ◽  
Vol 21 (21) ◽  
pp. 8019
Author(s):  
Parinaz Sabourian ◽  
Ghazaleh Yazdani ◽  
Seyed Sajad Ashraf ◽  
Masoud Frounchi ◽  
Shohreh Mashayekhan ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Surface Composition ◽  
Chemical Properties ◽  
Therapeutic Interventions ◽  
Morbidity Rate ◽  
High Morbidity ◽  
Structure Property ◽  
Transport Pathways ◽  
High Morbidity Rate ◽  
Physico Chemical

Cellular internalization of inorganic, lipidic and polymeric nanoparticles is of great significance in the quest to develop effective formulations for the treatment of high morbidity rate diseases. Understanding nanoparticle–cell interactions plays a key role in therapeutic interventions, and it continues to be a topic of great interest to both chemists and biologists. The mechanistic evaluation of cellular uptake is quite complex and is continuously being aided by the design of nanocarriers with desired physico-chemical properties. The progress in biomedicine, including enhancing the rate of uptake by the cells, is being made through the development of structure–property relationships in nanoparticles. We summarize here investigations related to transport pathways through active and passive mechanisms, and the role played by physico-chemical properties of nanoparticles, including size, geometry or shape, core-corona structure, surface chemistry, ligand binding and mechanical effects, in influencing intracellular delivery. It is becoming clear that designing nanoparticles with specific surface composition, and engineered physical and mechanical characteristics, can facilitate their internalization more efficiently into the targeted cells, as well as enhance the rate of cellular uptake.

Study on effect of custard apple leaf extract on physico-chemical properties of aonla juice

2016 ◽  
Vol 35 (1) ◽  
Author(s):  
Sushilkumar S. Mundhe ◽  
Arvind R. Sawate ◽  
Bhanudas M. Patil ◽  
Rajesh B. Kshirsagar ◽  
S. P. Kulkarni
Keyword(s):  
Leaf Extract ◽  
Chemical Properties ◽  
Distilled Water ◽  
Overall Acceptability ◽  
Liquid Extract ◽  
Physico Chemical ◽  
Plant Pigment ◽  
Custard Apple ◽  
Juice Sample ◽  
Spray Dried

The leaf extract was prepared by shade drying of leaves for 4-5 days, the dried leaves were pulverized and boiled in water bath with distilled water to get the liquid extract. Further the liquid extract was centrifuged and then supernatant was spray dried to get powdered extract. Sweetened aonla juice formulation was made with different proportion of custard apple leaf extract varying from 0 to 15 g in increasing order from control (0) to A1 (5g), A2 (10g) and A3 (15g) respectively. The prepared juices were analyzed for physico-chemical composition and sensory evaluation. The retention of quercetin [(a flavonol, is a flavonoid, in other words, a plant pigment with a molecular structure like flavone. It is found in fruits, vegetables, leaves and grains. It can be used as an ingredient in supplements, beverages, or foods having nutraceutical value.)] in aonla juice sample A<sub>1</sub>, A<sub>3</sub> was 15.20, 32.80 and 49.75 mg per 1000 ml. Sample A<sub>2</sub> (10g custard apple leaf A<sub>2</sub>, extract) scored highest score for overall acceptability was 8.8.

scholarly journals Engineering of Some Physico-Chemical Properties of Amorphous Spray-Dried Inulin using a Response-Surface Design

2020 ◽  
Vol 17 (2) ◽  
pp. 24-32
Author(s):  
Brahim Bchir ◽  
Sebastien N. Ronkart ◽  
Yves Brostaux ◽  
Lynn Doran ◽  
Christian Fougnies ◽  
...  
Keyword(s):  
Response Surface ◽  
Chemical Properties ◽  
Response Surface Design ◽  
Surface Design ◽  
Physico Chemical ◽  
Spray Dried

Decoration of specific sites on freeze-fractured membranes

1978 ◽  
Vol 36 (2) ◽  
pp. 140-141
Author(s):  
H. Gross ◽  
H. Moor
Keyword(s):  
Pure Water ◽  
Freeze Fracture ◽  
Chemical Properties ◽  
Surface Forces ◽  
Sulfate Pentahydrate ◽  
Copper Sulfate Pentahydrate ◽  
Physico Chemical ◽  
Water Of Hydration ◽  
Small Container ◽  
Binding Forces

Fracturing under ultrahigh vacuum (UHV, p ≤ 10-9 Torr) produces membrane fracture faces devoid of contamination. Such clean surfaces are a prerequisite foe studies of interactions between condensing molecules is possible and surface forces are unequally distributed, the condensate will accumulate at places with high binding forces; crystallites will arise which may be useful a probes for surface sites with specific physico-chemical properties. Specific “decoration” with crystallites can be achieved nby exposing membrane fracture faces to water vopour. A device was developed which enables the production of pure water vapour and the controlled variation of its partial pressure in an UHV freeze-fracture apparatus (Fig.1a). Under vaccum (≤ 10-3 Torr), small container filled with copper-sulfate-pentahydrate is heated with a heating coil, with the temperature controlled by means of a thermocouple. The water of hydration thereby released enters a storage vessel.

Sign in / Sign up

Export Citation Format

Share Document