scholarly journals RNAi inhibition of feruloyl CoA 6′-hydroxylase reduces scopoletin biosynthesis and post-harvest physiological deterioration in cassava (Manihot esculenta Crantz) storage roots

2017 ◽  
Vol 94 (1-2) ◽  
pp. 185-195 ◽  
Author(s):  
Shi Liu ◽  
Ima M. Zainuddin ◽  
Herve Vanderschuren ◽  
James Doughty ◽  
John R. Beeching
Keyword(s):  
Manihot Esculenta ◽  
Storage Roots ◽  
Manihot Esculenta Crantz ◽  
Post Harvest

scholarly journals Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0174238 ◽  
Author(s):  
Yuling Qin ◽  
Astride Stéphanie Mouafi Djabou ◽  
Feifei An ◽  
Kaimian Li ◽  
Zhaogui Li ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Manihot Esculenta ◽  
Storage Roots ◽  
Manihot Esculenta Crantz ◽  
Postharvest Physiological Deterioration

Expression patterns of the gene encoding starch branching enzyme II in the storage roots of cassava (Manihot esculenta Crantz)

Plant Science ◽  
2003 ◽  
Vol 164 (5) ◽  
pp. 833-839 ◽  
Author(s):  
Yona Baguma ◽  
Chuanxin Sun ◽  
Staffan Ahlandsberg ◽  
Joel Mutisya ◽  
Sara Palmqvist ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Expression Patterns ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Storage Roots ◽  
Gene Encoding ◽  
Manihot Esculenta Crantz

Glutamic acid-rich proteins in cassava (Manihot esculenta Crantz) storage roots

2013 ◽  
Vol 57 (6) ◽  
pp. 934-935 ◽  
Author(s):  
Cláudia Regina Batista de Souza ◽  
Luiz Joaquim Castelo Branco Carvalho
Keyword(s):  
Glutamic Acid ◽  
Manihot Esculenta ◽  
Storage Roots ◽  
Manihot Esculenta Crantz

scholarly journals Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz)

2020 ◽  
Author(s):  
Kun Pan ◽  
Cheng Lu ◽  
Peixian Nie ◽  
Meizhen Hu ◽  
Xincheng Zhou ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Early Stage ◽  
Population Level ◽  
Starch Accumulation ◽  
Storage Roots ◽  
Sucrose Transport ◽  
Phloem Unloading ◽  
Manihot Esculenta Crantz ◽  
Parenchyma Cells ◽  
Fibrous Roots

Abstract Background: It is well known that cassava (Manihot esculenta Crantz) highly efficiently accumulates starch in its storage roots, but how photosynthates are transported from the leaves to the phloem (especially how they are unloaded into parenchymal cells of storage roots) remains unclear. Methods: Here, we investigated the phloem unloading pattern of sucrose and its impact on the development of cassava storage roots through microstructural and physiological analyses and carboxyfluorescein (CF) and isotope C14 tracing. Expression profiling of genes involved in symplastic and apoplastic transport was performed, and their correlations with storage root yield were determined in populations according to enzyme activity, Western blotting analysis and transcriptome sequencing. Results: Carbohydrates are transported mainly in the form of sucrose, with more than 54.6% present in the stem phloem at any time. Sucrose was predominantly unloaded symplastically from the phloem into storage roots, but there was a shift from apoplastic to symplastic unloading accompanied by the onset of root swelling. Statistical data concerning the microstructure revealed an enrichment of plasmodesmata within sieve, companion and parenchyma cells in the developing storage roots of a cultivar but not in a wild ancestor. Tracing tests with CF verified the existence of a coplastid channel, and [14C]Suc demonstrated that the marked sucrose could rapidly diffuse into root parenchyma cells from phloem cells. The relatively high expression of genes encoding SuSy and associated proteins in the storage roots at the middle and late stages but not in the early stage of storage roots, primary fibrous roots or secondary fibrous roots and the inverse expression pattern of SUTs, CWI and SAI in these corresponding organs supported the presence of a symplastic sucrose unloading pathway. The transcriptome pattern of genes involved in symplastic unloading and their significantly positive correction with starch yield at the population level confirmed that symplastic sucrose transport is vitally important in the development of cassava storage roots. Conclusions: In this study, we revealed a predominantly symplastic phloem unloading pattern of sucrose in cassava storage roots. This pattern is essential for the efficient starch accumulation for sucrose transport between high-yielding varieties and low-yielding wild ancestors.

scholarly journals Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz)

2020 ◽  
Author(s):  
Kun Pan ◽  
Cheng Lu ◽  
Peixian Nie ◽  
Meizhen Hu ◽  
Xincheng Zhou ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Early Stage ◽  
Population Level ◽  
Starch Accumulation ◽  
Storage Roots ◽  
Sucrose Transport ◽  
Phloem Unloading ◽  
Manihot Esculenta Crantz ◽  
Parenchyma Cells ◽  
Fibrous Roots

Abstract Background: It is well known that cassava (Manihot esculenta Crantz) highly efficiently accumulates starch in its storage roots, but how photosynthates are transported from the leaves to the phloem (especially how they are unloaded into parenchymal cells of storage roots) remains unclear. Methods: Here, we investigated the phloem unloading pattern of sucrose and its impact on the development of cassava storage roots through microstructural and physiological analyses and carboxyfluorescein (CF) and isotope C14 tracing. Expression profiling of genes involved in symplastic and apoplastic transport was performed, and their correlations with storage root yield were determined in populations according to enzyme activity, Western blotting analysis and transcriptome sequencing. Results: Carbohydrates are transported mainly in the form of sucrose, with more than 54.6% present in the stem phloem at any time. Sucrose was predominantly unloaded symplastically from the phloem into storage roots, but there was a shift from apoplastic to symplastic unloading accompanied by the onset of root swelling. Statistical data concerning the microstructure revealed an enrichment of plasmodesmata within sieve, companion and parenchyma cells in the developing storage roots of a cultivar but not in a wild ancestor. Tracing tests with CF verified the existence of a coplastid channel, and [14C]Suc demonstrated that the marked sucrose could rapidly diffuse into root parenchyma cells from phloem cells. The relatively high expression of genes encoding SuSy and associated proteins in the storage roots at the middle and late stages but not in the early stage of storage roots, primary fibrous roots or secondary fibrous roots and the inverse expression pattern of SUTs, CWI and SAI in these corresponding organs supported the presence of a symplastic sucrose unloading pathway. The transcriptome pattern of genes involved in symplastic unloading and their significantly positive correction with starch yield at the population level confirmed that symplastic sucrose transport is vitally important in the development of cassava storage roots. Conclusions: In this study, we revealed a predominantly symplastic phloem unloading pattern of sucrose in cassava storage roots. This pattern is essential for the efficient starch accumulation for sucrose transport between high-yielding varieties and low-yielding wild ancestors.

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