Large-scale regeneration of hermaphrodite emblings of Carica papaya L. ‘Golden’ using early molecular sex determination during embryogenic callus multiplication

2021 ◽  
Author(s):  
Lucas Xavier ◽  
Felipe Astolpho Almeida ◽  
Vitor Batista Pinto ◽  
Vanildo Silveira
Keyword(s):  
Sex Determination ◽  
Embryogenic Callus ◽  
Large Scale ◽  
Carica Papaya ◽  
Scale Regeneration ◽  
Molecular Sex Determination

scholarly journals Application of RAPD Markers on Sex Determination of Papaya (Carica papaya)

2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Riry Prihatini ◽  
Noflindawati , ◽  
Tri Budiyanti
Keyword(s):  
Sex Determination ◽  
Dna Markers ◽  
Rapd Markers ◽  
Carica Papaya ◽  
Polymorphic Bands ◽  
Molecular Sex Determination ◽  
Random Amplified Polymorphism Dna

Molecular sex determination of five varieties of Indonesian papaya were investigated using RAPD (Random Amplified Polymorphism DNA) markers. Overall, 12 of 14 primers produced polymorphic bands on either several or all tested varieties. The OPC04 and RAPD2 markers could be used determined sex types on all varieties, whereas others primers are only on certain varieties. The Tangkai Ungu variety can be differentiate by markers: OPA11, OPA14, OPC14, RAPD2, RAPD3, and RAPD5; the Lokal Sumani can be determine using markers: OPA01, OPA11, OPA14, OPC01, OPC04, RAPD2, RAPD3, RAPD5, and RAPD6; the Merah Delima could be determined using OPC04, OPN09, RAPD2, and RAPD5; the Dampit could be determined using OPC01, OPC04, RAPD2, and RAPD6; whereas the Sicincing Panjang could be determined using OPA04, OPA11, OPA14, OPC04, RAPD2, and RAPD3.

Transcriptome-wide effect of DE-ETIOLATED1 (DET1) suppression in embryogenic callus of Carica papaya

2019 ◽  
Vol 132 (2) ◽  
pp. 181-195 ◽  
Author(s):  
Nur Diyana Jamaluddin ◽  
Emelda Rosseleena Rohani ◽  
Normah Mohd Noor ◽  
Hoe-Han Goh
Keyword(s):  
Embryogenic Callus ◽  
Carica Papaya

scholarly journals A dense linkage map for a large repetitive genome: discovery of the sex-determining region in hybridising fire-bellied toads (Bombina bombina and B. variegata)

2020 ◽  
Author(s):  
Beate Nürnberger ◽  
Stuart J.E. Baird ◽  
Dagmar Čížková ◽  
Anna Bryjová ◽  
Austin B. Mudd ◽  
...  
Keyword(s):  
Sex Determination ◽  
Linkage Map ◽  
Large Scale ◽  
Sex Chromosome ◽  
Repetitive Sequences ◽  
Secondary Contact ◽  
Gene Pools ◽  
Hybrid Zones ◽  
Chromosome 5 ◽  
Bombina Bombina

AbstractHybrid zones that result from secondary contact between diverged populations offer unparalleled insight into the genetic architecture of emerging reproductive barriers and so shed light on the process of speciation. Natural selection and recombination jointly determine their dynamics, leading to a range of outcomes from finely fragmented mixtures of the parental genomes that facilitate introgression to a situation where strong selection against recombinants retains large unrecombined genomic blocks that act as strong barriers to gene flow. In the hybrid zone between the fire-bellied toads Bombina bombina and B. variegata (Anura: Bombinatoridae), two anciently diverged and ecologically distinct taxa meet and produce abundant, fertile hybrids. The dense linkage map presented here enables genomic analysis of the selection-recombination balance that keeps the two gene pools from merging into one. We mapped 4,775 newly developed marker loci from bait-enriched genomic libraries in F2 crosses. The enrichment targets were selected from a draft assembly of the B. variegata genome, after filtering highly repetitive sequences. We developed a novel approach to infer the most likely diplotype per sample and locus from the raw read mapping data, which is robust to over-merging and obviates arbitrary filtering thresholds. Large-scale synteny between Bombina and Xenopus tropicalis supports the resulting linkage map. By assessing the sex of late-stage F2 tadpoles from histological sections, we also identified the sex-determining region in the Bombina genome to 7 cM on LG5, which is homologous to X. tropicalis chromosome 5, and inferred male heterogamety, suggestive of an XY sex determination mechanism. Interestingly, chromosome 5 has been repeatedly recruited as a sex chromosome in anurans with XY sex determination.

scholarly journals Partial Organogenesis and Somatic Embryogenesis from Petiole Explants of Field-grown Papaya (Carica papaya L.)

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629g-630 ◽  
Author(s):  
S. Jayasankar ◽  
U.L Yadava
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Carica Papaya ◽  
Vascular Tissue ◽  
The Other ◽  
Ms Medium ◽  
Free Medium ◽  
Petiole Explants ◽  
Young Leaves

Petiole discs from young leaves of female papaya (L-45) plants were cultured in MS or B5-based media containing 0, 2.25, 4.5, 11.25, and 22.5 μm 2,4-D. Compact embryogenic callus emerged from vascular tissue of petiole discs in about 3 weeks. In MS medium, 66% and 51% explants formed embryogenic callus with 11.25 and 22.5 μm 2,4-D, respectively. On the other hand, 79% explants formed embryogenic callus in B5-based medium with 4.50 μm 2,4-D. However, explants became necrotic in B5-based medium with 22.5 μm 2,4-D. Subculturing callus in auxin-free medium resulted in the development of roots or somatic embryos. Microscopic observations revealed that the roots were produced only by the callus that had retained its continuity with the vascular tissue. This investigation revealed that petioles from field grown papaya plants are potential explants for somatic embryogenesis and 2-week exposure to 2,4-D is adequate for inducing morphogenesis. Additionally, an interaction between 2,4-D and the components in the MS and B5-based media was observed.

scholarly journals Somatic Embryogenesis of the Cedars of Lebanon (Cedrus libani)

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 461G-462
Author(s):  
S.N. Talhouk ◽  
M. Shmoury ◽  
R. Baalbaki ◽  
S. Khuri
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Large Scale ◽  
Developmental Stages ◽  
Zygotic Embryos ◽  
Scale Production ◽  
Cedrus Libani ◽  
Hormone Levels ◽  
Significant Difference

Somatic embryogenesis offers a great potential for large-scale production of Cedrus libani, which is important not only as a forest tree, but also for the development of a timber industry. In an attempt to optimize conditions for embryogenic callus induction, we used zygotic embryos at different developmental stages as explants, compared different media, and used several hormone levels and combinations. Results indicated that post-cotyledonary immature embryos had highest induction efficiency. Four different media namely 1/2 MS, Durzan, Litvay's, and Von Arnold supplemented with similar hormone levels showed no significant difference in efficiency of callus induction. Induction frequencies of embryogenic callus from explants subjected to different hormone levels and combinations were dependent on the developmental stage of the explant.

scholarly journals Current Proteomic and Metabolomic Knowledge of Zygotic and Somatic Embryogenesis in Plants

2021 ◽  
Vol 22 (21) ◽  
pp. 11807
Author(s):  
Janet Juarez-Escobar ◽  
Esaú Bojórquez-Velázquez ◽  
Jose M. Elizalde-Contreras ◽  
José A. Guerrero-Analco ◽  
Víctor M. Loyola-Vargas ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Large Scale ◽  
Research Effort ◽  
Zygotic Embryogenesis ◽  
Potential Contribution ◽  
In Vitro Techniques ◽  
Current State ◽  
Scale Regeneration ◽  
State Of Research

Embryogenesis is the primary developmental program in plants. The mechanisms that underlie the regulation of embryogenesis are an essential research subject given its potential contribution to mass in vitro propagation of profitable plant species. Somatic embryogenesis (SE) refers to the use of in vitro techniques to mimic the sexual reproduction program known as zygotic embryogenesis (ZE). In this review, we synthesize the current state of research on proteomic and metabolomic studies of SE and ZE in angiosperms (monocots and dicots) and gymnosperms. The most striking finding was the small number of studies addressing ZE. Meanwhile, the research effort focused on SE has been substantial but disjointed. Together, these research gaps may explain why the embryogenic induction stage and the maturation of the somatic embryo continue to be bottlenecks for efficient and large-scale regeneration of plants. Comprehensive and integrative studies of both SE and ZE are needed to provide the molecular foundation of plant embryogenesis, information which is needed to rationally guide experimental strategies to solve SE drawbacks in each species.

Duplex PCR-based molecular sex determination of Saiga tatarica

2018 ◽  
Vol 12 (1) ◽  
pp. 21-23
Author(s):  
Xin Ding ◽  
Han Zheng ◽  
Kun Jin ◽  
Xuedong Liu
Keyword(s):  
Sex Determination ◽  
Duplex Pcr ◽  
Saiga Tatarica ◽  
Molecular Sex Determination

Molecular markers for sex determination in papaya (Carica papaya L.)

2002 ◽  
Vol 106 (1) ◽  
pp. 107-111 ◽  
Author(s):  
J. Deputy ◽  
R. Ming ◽  
H. Ma ◽  
Z. Liu ◽  
M. Fitch ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Carica Papaya
Sign in / Sign up

Export Citation Format

Share Document