The genetic identification of camel contagious ecthyma virus as the causative agent of contagious ecthyma in dromedary camels (Camelus dromedarius) in Qatar

2021 ◽  
Vol 53 (2) ◽  
Author(s):  
Amir A. Shehata ◽  
Ehab M. El-Nahas ◽  
Eman M. Abo Hatab ◽  
Saad S. A. Sharawi ◽  
Hanaa A. Ahmed
Keyword(s):  
Causative Agent ◽  
Camelus Dromedarius ◽  
Genetic Identification ◽  
Contagious Ecthyma ◽  
Dromedary Camels

scholarly journals Identification and phylogenetic analysis of contagious ecthyma virus from camels (Camelus dromedarius) in Iran

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
Ahmad Oryan ◽  
Mahboobe Mosadeghhesari ◽  
Saeed Zibaee ◽  
Ali Mohammadi
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
Camelus Dromedarius ◽  
Contagious Ecthyma ◽  
Dromedary Camels ◽  
Chain Reaction ◽  
B2l Gene ◽  
Bovine Papular Stomatitis Virus ◽  
Specific Amplification ◽  
Polymerase Chain

Contagious ecthyma is a highly contagious disease affecting domestic and wild ruminants such as sheep, goats and camels. The identification and characterisation of a parapoxvirus (PPV) infecting camels is described here. The virus was detected in dromedary camels (Camelus dromedarius) from Kerman and Shiraz in Iran. PPV-specific amplification by polymerase chain reaction (PCR) further confirmed that the disease was associated with PPV infection. Phylogenetic analysis of ORF011 (B2L) gene sequences showed 99.79% and 82.13% similarity of the PPV identified in this study with the Jodhpur isolate and the bovine papular stomatitis virus (BPSV) isolates (CE41), respectively. Moreover, phylogenetic analysis of the ORF045 gene indicated that the Shiraz sample was in all probability closely related to VR634 and to F00.120R and PCPV776. In conclusion, the results suggest that camel PPV (CPPV) is a likely cause of contagious ecthyma in dromedary camels in Iran.

Coxiella burnetii in Tunisian dromedary camels (Camelus dromedarius): Seroprevalence, associated risk factors and seasonal dynamics

Acta Tropica ◽  
2018 ◽  
Vol 188 ◽  
pp. 234-239 ◽  
Author(s):  
Rachid Selmi ◽  
Aymen Mamlouk ◽  
Houcine Ben Yahia ◽  
Hedi Abdelaali ◽  
Mourad Ben Said ◽  
...  
Keyword(s):  
Risk Factors ◽  
Coxiella Burnetii ◽  
Seasonal Dynamics ◽  
Camelus Dromedarius ◽  
Dromedary Camels ◽  
Associated Risk Factors

Prevalence of Cryptosporidium isolated from dromedary camels (Camelus dromedarius) in Qeshm Island, Southern Iran

2009 ◽  
Vol 19 (3) ◽  
pp. 311-314 ◽  
Author(s):  
S. Nazifi ◽  
M. A. Behzadi ◽  
SH. Haddadi ◽  
A. Raayat Jahromi ◽  
S. Mehrshad ◽  
...  
Keyword(s):  
Camelus Dromedarius ◽  
Dromedary Camels ◽  
Qeshm Island ◽  
Southern Iran

scholarly journals Genomic Analysis and Comparison of Pakistani Camels (Camelus dromedarius) by Prion Gene

2017 ◽  
Vol 45 (1) ◽  
pp. 8
Author(s):  
Tanveer Hussain ◽  
Muneeb Musthafa ◽  
Masroor Ellahi Babar ◽  
Faiz Marikar ◽  
Fiaz Hussain ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Prion Protein ◽  
Neurodegenerative Disorders ◽  
Genomic Dna ◽  
Protein Gene ◽  
Camelus Dromedarius ◽  
Prion Protein Gene ◽  
Dromedary Camels ◽  
Prion Gene ◽  
Bactrian Camels

Background: In many parts of the Old World, domesticated camels (genus - Camelus) are an essential resource, providing food, labor, commodities, and sport to millions of people Of the three extent species, two have been domesticated (singlehumped dromedarius, Camelus dromedarius, and two humped Bactrian camels Camelus bactrianus) and one remains wild (two-humped wild Bactrian camels Camelus ferus). All three species possess a variety adaptations to harsh desert conditions, including mechanisms to tolerate of extreme temperatures, dehydration, and sandy terrain. People residing in harsh climate zones of the world are being benefitted by raising camels in terms of draft, milk, meat, hides and wool from centuries. There are different breeds of dromedary camels distributed in various parts of Pakistan; however there have been scarcity of research work on camels in Pakistan. Identification of novel link between Camel breeders with fatal neurodegenerative disorders is presence or not can be detect by a Prion gene and it was not carried out in Pakistan soil to date. Prion diseases which are a group of fatal neurodegenerative disorders affect both animals and humans. It is believed that the prions are infectious agents responsible for transmissible spongiform encephalopathies. In this study we report the first study on Prion protein gene in dromedary camels of Pakistan.Material, Methods & Results: Genes are the blueprint of life and determine the functional aspects of cellular mechanisms. Genomic DNA of the enrolled blood samples was extracted using the Nucleospin® DNA extraction kit. Genomic DNA was run on Agarose gel electrophoresis, checked the Genomic DNA quality and amplified using prion region specific primer pair. Prion protein gene was amplified (770 bp) in 35 individuals of seven dromedary camel breeds from the province Balochistan and Punjab of Pakistan. Samples having required fragment size were selected and sent for sequencing through Sanger Sequencing. All the sequences were aligned through multiple sequence alignment and edited using Codon Code aligner and explored for phylogenetic analysis. A portion of 667 bp was finally selected for phylogenetic analysis of dromedary camels from Pakistan with 61 different mammalian species (drawn from GenBank) that revealed five different clades. We found 99.9% nucleotide sequence similarities among Dromedary camels (Germany), Dromedary camels (Iran), and Dromedary camels (Pakistan). We observed deletion in dromedary camels in codon region 66-69 except wild Bactrian camels that might be the causative factor for Prion protein gene resistance in camels. The Neighbor-Joining method with bootstrap analysis of 1000 replicates was used to draw phylogenetic tree.Discussion: This study documents the presence of 14 PrP polymorphisms and shows the relationship between different camel breeds. The deletion had not previously been examined PrP allelic variation, and was found to segregate in these breeds. However, additional data are necessary to demonstrate PrP and genetic approach will be ideal for the future studies and, more investigations are necessary to demonstrate PrP genetic resistance in camels. The utility of these techniques in identifying Prion genes and selecting superior animals and culling the weak animals and making them parents of the next generation that will lead to producing more meat and milk with fewer animals are well discussed and by slaughtering of high Prion incidence will eliminate human animal fatal neurodegenerative disorders.

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015 ◽  
Vol 83 (4) ◽  
pp. 650-654 ◽  
Author(s):  
M.M. Waheed ◽  
I.M. Ghoneim ◽  
A.K. Alhaider
Keyword(s):  
Seminal Plasma ◽  
Camelus Dromedarius ◽  
Dromedary Camels
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