Shoot meristem culture eliminates bacterial and fungal infections from elite varieties of turmeric (Curcuma longa L.)

2021 ◽  
Author(s):  
Cattarin Theerawitaya ◽  
Myo Myat Myat Kyaw ◽  
Thapanee Samphumphuang ◽  
Umpawa Pinruan ◽  
Natthawut Wiriyathanawudhiwong ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Curcuma Longa ◽  
Shoot Meristem ◽  
Meristem Culture

Analytical profile of Arka Taila - An Ayurvedic oil based medicine used in Karnasrava (Otomycosis)

2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Komal D. Jani ◽  
Dharmendra P. Jani
Keyword(s):  
Quality Control ◽  
Fungal Infections ◽  
Curcuma Longa ◽  
Calotropis Procera ◽  
Control Parameters ◽  
Scientific Evaluation ◽  
Quality Control Parameters ◽  
Quality Check ◽  
Based Medicine ◽  
Excessive Accumulation

Otomycosis denotes diffuse otitis externa due to fungal infections. The fungii are usually secondary invaders of the tissue rendered susceptible by bacterial infection, physical injury or excessive accumulation of cerumen in the external auditory canal. Karnasraava means discharge from ear which can be correlated with otorrhoea. Karnasraava (discharge) is one of symptom of otomycosis. Arka Taila has been mentioned in Shaarangadhara is very simple formula having ingredients i.e. Haridraa (Curcuma longa), Arka Patra Swarasa (Calotropis procera) and Sarshapa Taila (Brassica campetries). The quality control parameters resulted after scientific evaluation of Arka Taila can be used as reference standard for quality control or quality assurance of a pharmaceutical industry in order to have a proper quality check over its preparation and processing.

GROWTH INHIBITORY ACTIVITIES OF THE RHIZOME CRUDE EXTRACT OF Curcuma longa ON THE HUMAN PATHOGENIC FUNGUS Mucor circinelloides

2020 ◽  
Vol 65 (10) ◽  
pp. 82-91
Author(s):  
Phuong Nguyen Anh ◽  
Mai Le Thi Tuyet ◽  
Trung Trieu Anh
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Fungal Infections ◽  
Early Stage ◽  
Curcuma Longa ◽  
Pathogenic Fungus ◽  
Mucor Circinelloides ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Dilution Method

Mucormycosis is an uncommon but life-threatening invasive fungal infection, mostly occurs in immunocompromised patients. Lacking the appropriate antifungal drugs is one of the reasons that lead to difficulties in the management of mucormycosis. Curcuma longa has been used traditionally and widely to treat various diseases, including fungal infections. In the search for novel antifungal compounds from natural resources, we evaluated the effect of rhizome crude extract of C. longa on Mucor circinelloides – a causal agent of mucormycosis. The results of screening, using broth dilution method and agar-well diffusion method, showed that the C. longa extract exhibited promising antifungal activity against the fungus M. circinelloides. In liquid medium, C. longa extract decreased the ability of spore germination and the speed of hyphae formation of M. circinelloides decreased by up to approximately 70% and 90%, respectively. Besides, in a solid medium, the crude extract presented similar activity with amphotericin B (400 μg\mL) in decreasing the growth of M. circinelloides by nearly 77%. Moreover, the extract of C. longa also likely to induce the yeast-like type of growth of the dimorphic M. circinelloides in the early stage. These results suggest the plant could be a potential source for further study on biochemical components and the mechanism of its antifungal activity.

scholarly journals A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut

2016 ◽  
Vol 7 ◽  
Author(s):  
Bhavyashree U ◽  
Lakshmi Jayaraj K ◽  
Fayas TP ◽  
Muralikrishna KS ◽  
Sajini KK ◽  
...  
Keyword(s):  
Comparative Study ◽  
Scaling Up ◽  
Shoot Development ◽  
Shoot Meristem ◽  
Meristem Culture ◽  
Embryogenic Calli ◽  
Material Production ◽  
Planting Material ◽  
Direct Shoot

<p><strong>A protocol was standardized to maximize yields of embryogenic calli from shoot meristem culture of coconut. Three different shoot meristem excision methods were tested <em>viz</em>., excision of shoot meristem aseptically from <em>in vitro</em> germinated embryo after 10-12 days, excision of shoot meristem from <em>in vitro</em> germinated embryo subjected to GA3 treatment for five days and excision of shoot meristem from fresh embryo. The primary calli induction after 30 days of culture incubation for the three treatments were 21%, 27%  and 79% respectively.  Further, the primary calli formed from the shoot meristem excised from fresh embryo gave rise to 56% of embryogenic calli. The calli obtained from the shoot meristem which were excised from <em>in vitro</em> germinated embryo formed less percentage of embryogenic calli because of the presence of cotyledonary tissues which inhibited the multiplication of meristematic tissues. In the case of shoot meristem extracted from GA3-treated embryos, the percentage of non-embryogenic calli was more compared to the shoot meristem excised from fresh embryo. It was observed that the addition of GA3 in the initial stages of culture inhibited the formation of embryogenic calli and favored direct shoot development. Currently, the shoot meristem excised from fresh embryo is being employed for scaling up the planting material production from released varieties of coconut.</strong></p>

Shoot Meristem Culture

1994 ◽  
pp. 715-719
Author(s):  
Erin E. Irish
Keyword(s):  
Shoot Meristem ◽  
Meristem Culture

The Cytological Status of Plants Regenerated from Shoot-Meristem Culture of Pisum sativum L

1987 ◽  
Vol 98 (4) ◽  
pp. 306-311 ◽  
Author(s):  
Rina Ahmed ◽  
S. Dutta Gupta ◽  
P. D. Ghosh
Keyword(s):  
Pisum Sativum ◽  
Shoot Meristem ◽  
Meristem Culture ◽  
Pisum Sativum L

scholarly journals Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Muhammad Evy Prastiyanto ◽  
NI’MATUR ROHMAH ◽  
LESITA EFENDI ◽  
RAHMATIA ARIFIN ◽  
FANDHI ADI WARDOYO ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Fungal Infections ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Antifungal Activities

Abstract. Prastiyanto ME, Rohmah N, Efendi L, Arifin R, Wardoyo FA, Wilson W, Mukaromah AH, Dewi SS, Darmawati S. 2021. Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum. Biodiversitas 22: 1509-1513. Fungal infections have now become serious health issues. One of the strategies to avoid the problems of fungal infections is by using natural product from plants that are effective against many human pathogenic fungi. The study portrayed the use of the extracts of plant rhizomes as the alternatives to fight against number of human pathogenic fungi. This research aimed to investigate the antifungal activities of crude ethanol extract of five member of the family Zingiberaceae (Curcuma longa, Alpinia galanga Zingiber officinale. var. rubrum, Zingiber officinale var. officinarum and Zingiber officinale var. amarum), which are widely used as folk medicines against Candida albicans and Trichophyton rubrum. Crude ethanol extracts of five members of Zingiberaceae were evaluated for their antifungal activities and the results were calculated based on the zones of inhibition using the diffusion method. The extract showed antifungal activity against Candida. albicans in the agar well diffusion assay (10.2-27.1 mm inhibition diameter) and against T. rubrum (27.3-44.3 mm inhibition diameter). The data have revealed that all rhizomes have the potential to be developed as antifungal agents, particularly against C. albicans and T. rubrum. Studies on the antifungal activity against yeast-like (C. albicans) and filamentous (T. rubrum) can provide new information about the benefits of members Zingiberaceae as a source of natural antifungal. Researchers can select the type of rhizome that has more potential for further extraction to obtain pure compounds that can be used as antifungals.

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