Cloning of XET gene from Anthocephalus chinensis and its plant expression vector construction

2010 ◽  
Vol 12 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Sheng-jun Ma ◽  
Song-lin Zhu ◽  
Wei Li ◽  
Kun-xi Ouyang ◽  
Na Li ◽  
...  
Keyword(s):  
Expression Vector ◽  
Plant Expression ◽  
Plant Expression Vector ◽  
Vector Construction

Cloning of strawberry FaEtr2 gene and its plant expression vector construction for antisense RNA

2009 ◽  
Vol 3 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Chunli Song ◽  
Junlian Ma ◽  
Xia Tang ◽  
Zide Zhang ◽  
Pingping Zhou ◽  
...  
Keyword(s):  
Antisense Rna ◽  
Expression Vector ◽  
Plant Expression ◽  
Plant Expression Vector ◽  
Vector Construction

Cloning of strawberry Etr2 gene and its plant expression vector construction for RNA interference

2008 ◽  
Vol 136 ◽  
pp. S183
Author(s):  
Chunli Song ◽  
Junlian Ma ◽  
Xia Tang ◽  
Zide Zhang ◽  
Congzhi Zhao
Keyword(s):  
Rna Interference ◽  
Expression Vector ◽  
Plant Expression ◽  
Plant Expression Vector ◽  
Vector Construction

scholarly journals The Construction of Plant Expression Vector harbouring Carica Papaya L. WRKY Gene in Escherichia coli

2020 ◽  
Vol 28 (S2) ◽  
Author(s):  
Fauziah Abu Bakar ◽  
Pavitra Paramalingam ◽  
Kamariah Hasan
Keyword(s):  
Abiotic Stress ◽  
Stress Responses ◽  
Expression Vector ◽  
Carica Papaya ◽  
Sequencing Analysis ◽  
Wrky Gene ◽  
Limited Information ◽  
Abiotic Stress Response ◽  
Plant Expression ◽  
Plant Expression Vector

Carica papaya is a well-liked and economically important fruit with outstanding nutritional and medicinal values. Its susceptibility to abiotic stress which affects the growth and harvest, causes significant yield loss to farmers. In recent years, significant progress has been made to understand the genes that play critical roles in abiotic stress response, especially some transcription factor (TF) encoding genes. Among all TFs, WRKY TF gene family is one of the best-studied TFs involved in various stress responses. To date, only limited information on functionally characterised WRKY TFs is available for C. papaya. The aim of this study was to produce a recombinant construct harbouring WRKY gene in pGEM®-T Easy cloning vector. The presence of a DNA band of the expected size of 465 bp on agarose gel electrophoresis indicated that WRKY gene was successfully amplified from all treated samples. DNA sequencing analysis revealed that the amplified sequence isolated from the treated samples were closely related to Carica papaya species with 97% similarity. Following transformation, 4 out of 5 colonies that were randomly selected showed the WRKY gene had been successfully inserted into pGEM®-T Easy vector and transformed into E. coli. In future, the WRKY gene from pGEMT-WRKY recombinant construct will be cloned into the plant expression vector pCAMBIA 1304 prior to transformation in the plant. The success of demonstrating the WRKY gene towards the response in abiotic stress will enable us to produce stress tolerant transgenic crops under unfavourable conditions via genetic engineering for sustained growth.

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