Characterization of inositol phospholipids and identification of a mastoparan-induced polyphosphoinositide response in Tetrahymena pyriformis

Дана биохимическая характеристика образцов зерна полбы кубанской селекции. Приведены результаты исследования биологической ценности этих образцов с использованием инфузории Tetrahymena pyriformis. Определены качественный и количественный составы поверхностной микрофлоры различных частей зерновки. Проведена барогидротермическая обработка (БГТО) зерна, и определено ее влияние на технологические и биологические свойства зерна полбы. Предложены и апробированы режимы БГТО образцов зерна полбы, увлажненного до 20, 25 и 30%, с отволаживанием зерна в течение 40 мин и последующим автоклавированием при избыточном давлении 0,1 МПа в течение 3, 5 и 10 мин. Установлено, что БГТО позволяет дезактивировать поверхностную микрофлору зерна полбы. Выявлено положительное влияние БГТО на увеличение биологической ценности зерна. Сравнительная оценка кулинарных свойств нативных зерен полбы и зерен полбы после БГТО показала сокращение времени варки крупы на 20 мин – с 60 до 40 мин. Biochemical characterization of grains einkorn Kuban breeding was given. The results of the study of the biological value of these samples with using Tetrahymena pyriformis are given. The qualitative and quantitative compositions of the surface microflora of different parts of the grain are determined. Barohydrothermal processing (BHTP) of grain is carried out, and its influence on technological and, biological of grain einkorn is defined. Regimes of BHTP samples of the grain einkorn moistened to 20, 25 and 30%, with the braving of the grain for 40 min and subsequent autoclaving at an excess pressure of 0,1 MPa within 3, 5 and 10 min are proposed and tested. It is established that BHTP allows to deactivate the surface microflora of the grain einkorn. Positive influence BHTP for increase the biological value of grain einkorn is revealed. A comparative assessment of the culinary properties of native grains einkorn and grains einkorn after BHTP showed a reduction in the time of cooking cereals by 20 min (from 60 to 40 min).

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Separation and Characterization of Inositol Phospholipids from the Pulvini of Samanea saman

PLANT PHYSIOLOGY ◽

10.1104/pp.90.4.1422 ◽

1989 ◽

Vol 90 (4) ◽

pp. 1422-1428 ◽

Cited By ~ 23

Author(s):

Gary G. Coté ◽

Anthony L. DePass ◽

Lynne M. Quarmby ◽

Bonnie F. Tate ◽

M. J. Morse ◽

...

Keyword(s):

Inositol Phospholipids ◽

Samanea Saman

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Characterization of agonist-stimulated incorporation of myo-[3H]inositol into inositol phospholipids and [3H]inositol phosphate formation in tracheal smooth muscle

Biochemical Journal ◽

10.1042/bj2620739 ◽

1989 ◽

Vol 262 (3) ◽

pp. 739-746 ◽

Cited By ~ 32

Author(s):

E R Chilvers ◽

P J Barnes ◽

S R Nahorski

Keyword(s):

Smooth Muscle ◽

Inositol Phosphate ◽

Specific Radioactivity ◽

Tracheal Smooth Muscle ◽

Muscarinic Agonist ◽

Dose Response Relationship ◽

Similar Extent ◽

Inositol Phospholipids ◽

Selective Depletion

The effects of the muscarinic agonist carbachol, histamine and bradykinin on incorporation of [3H]inositol into the phosphoinositides and the formation of [3H]InsPs were examined in bovine tracheal smooth-muscle (BTSM) slices labelled with [3H]inositol. These agonists result in substantial and dose-related increases in the incorporation of [3H]inositol into the phospholipids. Carbachol and histamine stimulated the incorporation of [3H]inositol into the phospholipids to the same degree, despite histamine being only 35% as effective as carbachol on [3H]InsP accumulation. Histamine and carbachol, at maximal concentrations, were non-additive with respect to both the stimulated incorporation of [3H]inositol and [3H]InsP formation. For carbachol this effect on incorporation was found to occur to a similar extent in PtdInsP and PtdInsP2 as well as PtdIns. The initial effect of carbachol on [3H]inositol incorporation was rapid (maximal by 10 min); however, with prolonged stimulation large secondary declines in PtdInsP and PtdInsP2 labelling were observed, with depletion of the much larger PtdIns pool only evident in the presence of Li+. Lowering buffer [Ca2+] increased the incorporation of [3H]inositol under basal conditions, but did not attenuate the subsequent agonist-stimulated incorporation effect. The large changes in specific radioactivity of the phosphoinositides, and consequently the [3H]InsP products, after carbachol stimulation resulted in the apparent failure of atropine to reverse the [3H]InsP response completely. Labelling muscle slices with [3H]inositol in the presence of carbachol or labelling for longer periods (greater than 6 h) prevented subsequent carbachol-stimulated effects on incorporation without significantly altering the dose-response relationship for carbachol-stimulated [3H]InsP formation and resulted in steady-state labelling conditions confirmed by the ability of atropine to reverse fully the [3H]InsP response to carbachol. This study demonstrates the profound effects of a number of agonists on [3H]inositol incorporation into the phospho- and polyphosphoinositides in BTSM with important consequent changes in the specific radioactivity of these lipids and the resulting [3H]InsP products. In addition, a selective depletion of PtdInsP and PtdInsP2 over PtdIns has been demonstrated with prolonged muscarinic-receptor stimulation.

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