scholarly journals Correction to: Molecular identification of Leishmania spp. isolates causes cutaneous leishmaniasis (CL) in Sanliurfa Province, Turkey, where CL is highly endemic

2018 ◽  
Vol 63 (3) ◽  
pp. 361-361
Author(s):  
Gulcan Gurses ◽  
Mehmet Ozaslan ◽  
Fadile Yıldız Zeyrek ◽  
Ibrahim H. Kılıç ◽  
Nebiye Yentür Doni ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Identification ◽  
Leishmania Spp

Molecular identification of Leishmania spp. isolates causes cutaneous leishmaniasis (CL) in Sanliurfa Province, Turkey, where CL is highly endemic

2017 ◽  
Vol 63 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Gulcan Gurses ◽  
Mehmet Ozaslan ◽  
Fadile Yıldız Zeyrek ◽  
Ibrahim H. Kılıç ◽  
Nebiye Yentür Doni ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Identification ◽  
Leishmania Spp

Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran

2020 ◽  
Author(s):  
Gilda ESLAMI ◽  
Ali FATTAHI BAFGHI ◽  
Mohammad Hassan LOTFI ◽  
Farzaneh MIRZAEI ◽  
Somayeh AHMADI ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Identification ◽  
Rflp Analysis ◽  
Cross Sectional Study ◽  
Central Iran ◽  
Endemic Region ◽  
Cross Sectional ◽  
Major Health Problem ◽  
Pcr Rflp ◽  
Leishmania Spp

Background: Cutaneous Leishmaniasis (CL) is a major health problem in many parts of Iran. Many methods have been introduced for detection and identification of Cutaneous Leishmaniasis. The purpose of this study was isolation and molecular identification of Leishmania spp. agents in patients with CL from endemic region of central Iran. In this study, one of the main loci of central Iran named Yazd will be assessed CL identification using PCR-RFLP. Methods: For this cross-sectional study, sampling was done from 372 suspicious patients with CL who referred to Health Centers of Yazd Province from 2016 to 2017. After collection samples of patients, DNA extraction was done from samples on slides. Genus detection was done using specific primers by PCR. RFLP analysis was done for species identification. Results: Out of 372 samples, 159 samples were positive using PCR based method. Out of 159 samples, 87 (54.7%) L. major and 72 (45.3%) L. tropica were identified using RFLP analysis. The number of lesions in each patient was different but 119 (74.8%) patients showed the number of 1-3 lesions, and more lesions (more than 10 lesions) was showed in 4 (2.5%) person. Conclusion: The CL found in Yazd province resulted from L. major and L. tropica as the agents of rural and urban types, respectively. The prevalence of L. major and L. tropica was almost the same. This indicated that control programs could be designed for treatment and vector and reservoir control.

scholarly journals Molecular Identification of Leishmania spp. DNA from Archived Giemsa-Stained Slides of Patients from Salta, Argentina

2018 ◽  
Vol 99 (5) ◽  
pp. 1156-1161 ◽  
Author(s):  
María Cristina Almazán ◽  
Paola Andrea Barroso ◽  
Alejandro Javier Krolewiecki ◽  
Julio Rubén Nasser ◽  
José Fernando Gil ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Leishmania Spp

scholarly journals Molecular identification of the parasites causing cutaneous leishmaniasis on the Caribbean coast of Colombia

2010 ◽  
Vol 106 (3) ◽  
pp. 647-652 ◽  
Author(s):  
Lily Paola Martínez ◽  
Juan Alberto Rebollo ◽  
Arturo Luis Luna ◽  
Suljey Cochero ◽  
Eduar Elías Bejarano
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Identification ◽  
Caribbean Coast ◽  
The Caribbean

scholarly journals Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi

Biomédica ◽  
2017 ◽  
Vol 37 ◽  
pp. 83 ◽  
Author(s):  
Jazzmín Arrivillaga-Henríquez ◽  
Sandra Enríquez ◽  
Vanessa Romero ◽  
Gustavo Echeverría ◽  
Jorge Pérez-Barrera ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Leishmania Spp

Introducción. La provincia de Pichincha, Ecuador, es un área endémica de leishmaniasis cutánea, en donde se han determinado como vectores los flebotomíneos antropofílicos con infección natural por Leishmania spp. Sin embargo, no se ha evaluado el papel en la transmisión de las especies zoofílicas.Objetivo. Evaluar la infección natural por Leishmania en dos especies de flebotomíneos zoofílicos, Lutzomyia reburra y Lu. barrettoi majuscula, y en una antropofílica, Lu. trapidoi, así como la endofagia y la sinantropía de estas especies en el noroccidente de Pichincha.Materiales y métodos. Los flebotomíneos se recolectaron en trampas de luz CDC colocadas en diferentes hábitats y altitudes en sitios que son focos de leishmaniasis cutánea. La infección con Leishmania spp. se detectó en el ADN genómico de hembras de las especies de flebotomíneos de interés. Se amplificó el gen espaciador interno de la transcripción del ARN ribosómico, unidad I (ITS1), y los genes de las topoiso-merasas mitocondrial II (mtTOPOII) y nuclear II (TopoII). Se determinaron los porcentajes de positividad para Leishmania a escala espaciotemporal, la proporción de endofagia y el índice de sinantropía.Resultados. Se determinó la presencia de infección natural por Le. amazonensis en Lu. reburra (9,5 %) y Lu. b. majuscula (23,8 %); en Lu. trapidoi se detectaron Le. amazonensis, Le. brazilienis y Le. naiffi-lainsoni. Los flebotomíneos eran asinantrópicos y con baja endofagia.Conclusión. Se registró por primera vez la presencia de infección natural en Lu. reburra y Lu. barrettoi majuscula por Le. amazonensis, y se demostró la importancia de los flebotomíneos zoofílicos en el mantenimiento del ciclo de transmisión de Leishmania spp. en focos endémicos.

scholarly journals Phlebotomines in an area endemic for American cutaneous leishmaniasis in northeastern coast of Brazil

2017 ◽  
Vol 26 (3) ◽  
pp. 280-284 ◽  
Author(s):  
Ana Patricia Oliveira da Silva ◽  
Débora Elienai de Oliveira Miranda ◽  
Marcos Antônio Bezerra Santos ◽  
Neurisvan Ramos Guerra ◽  
Silvia Rafaelli Marques ◽  
...  
Keyword(s):  
Disease Control ◽  
Cutaneous Leishmaniasis ◽  
Abundant Species ◽  
Risk Of Infection ◽  
Light Traps ◽  
Rainy Period ◽  
Worldwide Distribution ◽  
American Cutaneous Leishmaniasis ◽  
Leishmania Spp ◽  
Northeastern Coast

Abstract Phlebotomines have worldwide distribution with many species present in Brazil, including the northeastern region, where the fauna is very diverse. The aim of this study was to identify the sandfly fauna in an area endemic for American cutaneous leishmaniasis (ACL) in the state of Pernambuco. Sandflies were caught on three consecutive nights every month from October 2015 to September 2016, from 5 pm to 5 am, using seven light traps of Centers for Disease Control (CDC) type. Females were identified and used for molecular Leishmania detection. A total of 2,174 specimens belonging to ten species were collected: Lutzomyia choti (88.2%; 1,917/2,174) was the most abundant species, followed by Lutzomyia whitmani (8.1%; 176/2,174) and Lutzomyia sordellii (1.5%; 33/2,174). The majority of the specimens were collected in peridomestic areas (64.1%; 1,394/2,174) and during the rainy period. All the samples examined were negative for Leishmania spp. The presence of Lutzomyia whitmani indoors and in peridomestic areas indicates that the inhabitants of this area are exposed to the risk of infection by the parasites responsible for ACL.

scholarly journals DETECTION OF PRO- INFLAMMATORY INTERLEUKIN 17 IN PATIENTS WITH L. TROPICA IN THI-QAR PROVINCE, IRAQ

2020 ◽  
Vol 17 (36) ◽  
pp. 949-957
Author(s):  
Mohammed Hassan FLAIH ◽  
Fadhil Abbas AL-ABADY ◽  
Khwam Reissan HUSSEIN
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Nested Pcr ◽  
Accurate Method ◽  
Leishmania Species ◽  
Clinical Samples ◽  
Interleukin 17 ◽  
Infected Female ◽  
Endemic Diseases ◽  
Leishmania Spp ◽  
South Of Iraq

Cutaneous leishmaniasis (CL) is a widespread health problem and considered one of the endemic diseases in Iraq. The dermal lesion occurs due to an obligate intracellular Leishmania parasite, which transmits by the bite of the infected female sandfly. This study aims to identify Leishmania species in Thi-Qar province/South of Iraq and detect IL-17 level in serum of infected patients with L. tropica. The study was conducted in three local locations, Al-Hussein Teaching, Al-Suq Al-Shyokh General, and Al-Shatrah General Hospitals in the province for the period from the beginning of November 2018 to the end of October 2019. After clinical diagnosis, eighty out of two hundred forty-seven samples were selected for molecular examination by nested-PCR technique, where the lesion edge was injected by normal saline and pulled again to obtain the parasite DNA. Also, a measure of the IL-17 concentration level in serum of the patients with ELISA. The findings of the electrophoresis of the kinetoplast minicircle DNA gene showed that 65 samples were positive for cutaneous leishmaniasis, and observed two species of Leishmania spp. in the study area, 46 (57.5%) samples were L. tropica at 750 bp and 19 (23.75%) samples were L. major. Serum IL-17 concentration recorded a significant increase among patients infected with L. tropica at different infection stages than control samples. Generally, the Nested-PCR technique is an accurate method for diagnosing clinical samples and molecular determination of Leishmania parasites. L. tropica is the dominant specie that caused CL in Thi-Qar province, while L. major recorded a low incidence.

scholarly journals Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Iso¬lates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran

2019 ◽  
Author(s):  
Yasaman Alijani ◽  
Saeedeh Sadat Hosseini ◽  
Salman Ahmadian ◽  
Sonia Boughattas ◽  
Gilda Eslami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cutaneous Leishmaniasis ◽  
Molecular Characterization ◽  
Significance Level ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Sensitive Isolate ◽  
The One

Background: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is neces­sary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecu­lar analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for find­ing the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in re­sistant isolates.

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