Leishmania spp. in Iraq. Electrophoretic isoenzyme patterns. II. Cutaneous leishmaniasis

1980 ◽  
Vol 74 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Tarik I. Aljeboori ◽  
D.A. Evans
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Spp ◽  
Isoenzyme Patterns

scholarly journals Correction to: Molecular identification of Leishmania spp. isolates causes cutaneous leishmaniasis (CL) in Sanliurfa Province, Turkey, where CL is highly endemic

2018 ◽  
Vol 63 (3) ◽  
pp. 361-361
Author(s):  
Gulcan Gurses ◽  
Mehmet Ozaslan ◽  
Fadile Yıldız Zeyrek ◽  
Ibrahim H. Kılıç ◽  
Nebiye Yentür Doni ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Identification ◽  
Leishmania Spp

scholarly journals Phlebotomines in an area endemic for American cutaneous leishmaniasis in northeastern coast of Brazil

2017 ◽  
Vol 26 (3) ◽  
pp. 280-284 ◽  
Author(s):  
Ana Patricia Oliveira da Silva ◽  
Débora Elienai de Oliveira Miranda ◽  
Marcos Antônio Bezerra Santos ◽  
Neurisvan Ramos Guerra ◽  
Silvia Rafaelli Marques ◽  
...  
Keyword(s):  
Disease Control ◽  
Cutaneous Leishmaniasis ◽  
Abundant Species ◽  
Risk Of Infection ◽  
Light Traps ◽  
Rainy Period ◽  
Worldwide Distribution ◽  
American Cutaneous Leishmaniasis ◽  
Leishmania Spp ◽  
Northeastern Coast

Abstract Phlebotomines have worldwide distribution with many species present in Brazil, including the northeastern region, where the fauna is very diverse. The aim of this study was to identify the sandfly fauna in an area endemic for American cutaneous leishmaniasis (ACL) in the state of Pernambuco. Sandflies were caught on three consecutive nights every month from October 2015 to September 2016, from 5 pm to 5 am, using seven light traps of Centers for Disease Control (CDC) type. Females were identified and used for molecular Leishmania detection. A total of 2,174 specimens belonging to ten species were collected: Lutzomyia choti (88.2%; 1,917/2,174) was the most abundant species, followed by Lutzomyia whitmani (8.1%; 176/2,174) and Lutzomyia sordellii (1.5%; 33/2,174). The majority of the specimens were collected in peridomestic areas (64.1%; 1,394/2,174) and during the rainy period. All the samples examined were negative for Leishmania spp. The presence of Lutzomyia whitmani indoors and in peridomestic areas indicates that the inhabitants of this area are exposed to the risk of infection by the parasites responsible for ACL.

scholarly journals DETECTION OF PRO- INFLAMMATORY INTERLEUKIN 17 IN PATIENTS WITH L. TROPICA IN THI-QAR PROVINCE, IRAQ

2020 ◽  
Vol 17 (36) ◽  
pp. 949-957
Author(s):  
Mohammed Hassan FLAIH ◽  
Fadhil Abbas AL-ABADY ◽  
Khwam Reissan HUSSEIN
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Nested Pcr ◽  
Accurate Method ◽  
Leishmania Species ◽  
Clinical Samples ◽  
Interleukin 17 ◽  
Infected Female ◽  
Endemic Diseases ◽  
Leishmania Spp ◽  
South Of Iraq

Cutaneous leishmaniasis (CL) is a widespread health problem and considered one of the endemic diseases in Iraq. The dermal lesion occurs due to an obligate intracellular Leishmania parasite, which transmits by the bite of the infected female sandfly. This study aims to identify Leishmania species in Thi-Qar province/South of Iraq and detect IL-17 level in serum of infected patients with L. tropica. The study was conducted in three local locations, Al-Hussein Teaching, Al-Suq Al-Shyokh General, and Al-Shatrah General Hospitals in the province for the period from the beginning of November 2018 to the end of October 2019. After clinical diagnosis, eighty out of two hundred forty-seven samples were selected for molecular examination by nested-PCR technique, where the lesion edge was injected by normal saline and pulled again to obtain the parasite DNA. Also, a measure of the IL-17 concentration level in serum of the patients with ELISA. The findings of the electrophoresis of the kinetoplast minicircle DNA gene showed that 65 samples were positive for cutaneous leishmaniasis, and observed two species of Leishmania spp. in the study area, 46 (57.5%) samples were L. tropica at 750 bp and 19 (23.75%) samples were L. major. Serum IL-17 concentration recorded a significant increase among patients infected with L. tropica at different infection stages than control samples. Generally, the Nested-PCR technique is an accurate method for diagnosing clinical samples and molecular determination of Leishmania parasites. L. tropica is the dominant specie that caused CL in Thi-Qar province, while L. major recorded a low incidence.

scholarly journals Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Iso¬lates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran

2019 ◽  
Author(s):  
Yasaman Alijani ◽  
Saeedeh Sadat Hosseini ◽  
Salman Ahmadian ◽  
Sonia Boughattas ◽  
Gilda Eslami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cutaneous Leishmaniasis ◽  
Molecular Characterization ◽  
Significance Level ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Sensitive Isolate ◽  
The One

Background: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is neces­sary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecu­lar analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for find­ing the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in re­sistant isolates.

scholarly journals Molecular Detection of Leishmania major in Hemiechinus auritus, A Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in Damghan, Iran

2019 ◽  
Author(s):  
Behrad Pourmohammadi ◽  
Sadegh Mohammadi-Azni
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Natural Infection ◽  
Leishmania Infection ◽  
Northern Iran ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Potential Reservoir ◽  
Leishmania Spp ◽  
First Time ◽  
Leishmania Parasites

Background: Zoonotic cutaneous leishmaniasis caused by Leishmania major is endemic in 17 of 31 Iranian provinces. Various species of rodents have been introduced as the main reservoirs of the disease. This study was conducted to de­termine the natural infection of hedgehogs with Leishmania spp. in an endemic area of the disease, northern Iran. Methods: Fifteen long-eared hedgehogs were captured alive during 18 months study period, from Apr 2015 to Sep 2016, in Damghan City, Semnan Province, Iran. The animals were identified using apparent characteristics and to de­termine the Leishmania infection, impression smears were prepared from their ear lobes, hind feet, livers, and spleens. Micro­scopic examination and semi-nested PCR were applied to determine the infection and to identify the parasites species respectively. Results: All examined animals were identified as Hemiechinus auritus (Family: Erinaceidae). In microscopic examina­tion, 8 (53.3%) samples were shown to be infected with Leishmania parasites. The higher and lower rate of the infection was observed in the ears as well as the feet and in the liver specimens, 53.3%, and 33.3% respectively. Forty percent (6/ 15) of the samples were molecularly positive and all were identified as L. major parasites. All the examined animals in au­tumn and 50% of them in summer were shown to be infected with Leishmania parasites. Conclusion: This study demonstrated the natural infection of H. auritus with L. major for the first time in Damghan City and introduced these mammals as new potential reservoirs of ZCL in the study area.

scholarly journals A Functional Wound Dressing as a Potential Treatment for Cutaneous Leishmaniasis

Pharmaceutics ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 200 ◽  
Author(s):  
Francisco Alexandrino-Junior ◽  
Kattya Gyselle de Holanda e Silva ◽  
Marjorie Caroline Liberato Cavalcanti Freire ◽  
Viviane de Oliveira Freitas Lione ◽  
Elisama Azevedo Cardoso ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Topical Treatment ◽  
Healing Process ◽  
Water Vapor Permeability ◽  
Vero Cells ◽  
Poly Vinyl Alcohol ◽  
Vapor Permeability ◽  
Secondary Infections ◽  
Leishmania Spp

Cutaneous leishmaniasis (CL) is a parasitic disease characterized by progressive skin sores. Currently, treatments for CL are limited to parenteral administration of the drug, which presents severe adverse effects and low cure rates. Therefore, this study aimed to develop poly(vinyl-alcohol) (PVA) hydrogels containing Amphotericin B (AmB) intended for topical treatment of CL. Hydrogels were evaluated in vitro for their potential to eliminate promastigote forms of Leishmania spp., to prevent secondary infections, to maintain appropriate healing conditions, and to offer suitable biocompatibility. AmB was incorporated into the system in its non-crystalline state, allowing it to swell more and faster than the system without the drug. Furthermore, the AmB release profile showed a continuous and controlled behavior following Higuchi´s kinetic model. AmB-loaded-PVA-hydrogels (PVA–AmB) also showed efficient antifungal and leishmanicidal activity, no cytotoxic potential for VERO cells, microbial impermeability and water vapor permeability compatible with the healthy skin’s physiological needs. Indeed, these results revealed the potential of PVA–AmB to prevent secondary infections and to maintain a favorable environment for the healing process. Hence, these results suggest that PVA–AmB could be a suitable and efficient new therapeutic approach for the topical treatment of CL.

scholarly journals Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis

2009 ◽  
Vol 104 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Zélia Maria Profeta Luz ◽  
Alexandre Rotondo da Silva ◽  
Fernanda de Oliveira Silva ◽  
Rachel B Caligiorne ◽  
Edward Oliveira ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Spp

Isolation and Molecular Diagnosis of Leishmania major and Study Activity of Aqueous Extract of Plant Nigella sativa Against the Parasite In Vitro

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Enas Abdul Kareem Jabbar ◽  
Bassad A. AL-Aboody ◽  
Basim Abdulhussein Jarullah ◽  
Nada Noori
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Molecular Diagnosis ◽  
Aqueous Extract ◽  
Teaching Hospital ◽  
Exposure Time ◽  
Leishmania Major ◽  
Nigella Sativa ◽  
Study Activity ◽  
Leishmania Spp

The current study included isolating and diagnosing the cutaneous leishmaniasis parasite from patients with Baghdad boil and the reviewers of Al Hussein Teaching Hospital in Thi-Qar province for the period from November to December 2016 and then studying the effect of aqueous extract of the plant Nigella sativa in the vitality of isolated parasite in vitro. Through the results, the isolated parasite was diagnosed depend on lesions appearance and characterization then confirm the diagnosis by KDNA- PCR which revealed that leishmania spp. was Leishmania major . In this study three different concentrations from aqueous extract of the Nigella sativa plant were used (50, 100 and 150 mg / ml), the parasite growing in NNN medium (3 replicates used)It was observed that the percentage of parasite destruction increased with prolong exposure time of the extract and increased concentration of the extract, results also exhibited that the percentage of the destruction was 100% after four hours of exposure to the concentration of 150 mg / ml which was the highly killed percentage compared to control, which was 5. 98%.

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