A first report on competitive inhibition of laccase enzyme by lignin degradation intermediates

2019 ◽  
Vol 65 (2) ◽  
pp. 431-437
Author(s):  
Sirisha Pamidipati ◽  
Asma Ahmed
Keyword(s):  
Lignin Degradation ◽  
Competitive Inhibition ◽  
First Report ◽  
Laccase Enzyme ◽  
Degradation Intermediates

scholarly journals First Report of Seroprevalence and Risk Factors ofNeospora caninumInfection in Tibetan Sheep in China

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Lan-Bi Nie ◽  
Wei Cong ◽  
Yang Zou ◽  
Dong-Hui Zhou ◽  
Qin-Li Liang ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Statistical Significance ◽  
Protozoan Parasite ◽  
Gansu Province ◽  
Serum Samples ◽  
First Report ◽  
Tibetan Sheep ◽  
Production Areas ◽  
And Control

Neospora caninumis an intracellular protozoan parasite which can cause abortion and stillbirth in ruminants. However, there is no information on Tibetan sheepN. caninuminfection in China. A total of 2187 serum samples were collected from Tibetan sheep in the major production areas of Luqu, Maqu, and Tianzhu in Gansu province, and Nyingchi in southeast Tibet, China. All samples were analyzed for the presence of antibodies toN. caninumusing a competitive-inhibition enzyme-linked immunoassay. Of the 2187 serum samples, 184 (8.4%, 95% CI 7.3-9.6) were testedN. caninumseropositive. TheN. caninumseroprevalence ranged from 4.4% (95% CI 1.4–7.4) to 11.3% (95% CI 8.2–14.4) among different regions, seasons, ages, and pregnancies, and there was no statistical significance among those groups (P> 0.05). Seroprevalence in male (10.8% 69/638) (95% CI 8.4–13.2) was significantly higher than in female (7.4% 115/1549) (OR =1.51, 95% CI 6.1–8.7) (P< 0.01). To our knowledge, this is the first report ofN. caninumseroprevalence in Tibetan sheep in China, which provides baseline data for the prevention and control ofN. caninuminfection in Tibetan sheep.

scholarly journals Four Novel Phenanthrene Derivatives with α-Glucosidase Inhibitory Activity from Gastrochilus bellinus

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 418
Author(s):  
Htoo Tint San ◽  
Nutputsorn Chatsumpun ◽  
Thaweesak Juengwatanatrakul ◽  
Natapol Pornputtapong ◽  
Kittisak Likhitwitayawuid ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Kinetic Study ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Competitive Inhibition ◽  
Spectroscopic Methods ◽  
First Report ◽  
Ic50 Value ◽  
Phytochemical Constituents

Four new phenanthrene derivatives, gastrobellinols A-D (1–4), were isolated from the methanolic extract of Gastrochilus bellinus (Rchb.f.) Kuntze, along with eleven known phenolic compounds including agrostophyllin (5), agrostophyllidin (6), coniferyl aldehyde (7), 4-hydroxybenzaldehyde (8), agrostophyllone (9), gigantol (10), 4-(methoxylmethyl)phenol (11), syringaldehyde (12), 1-(4′-hydroxybenzyl)-imbricartin (13), 6-methoxycoelonin (14), and imbricatin (15). Their structures were determined by spectroscopic methods. Each isolate was evaluated for α-glucosidase inhibitory activity. Compounds 1, 2, 3, 7, 9, 13, and 15 showed higher activity than the drug acarbose. Gastrobellinol C (3) exhibited the strongest α-glucosidase inhibition with an IC50 value of 45.92 μM. A kinetic study of 3 showed competitive inhibition on the α-glucosidase enzyme. This is the first report on the phytochemical constituents and α-glucosidase inhibitory activity of G. bellinus.

scholarly journals Optimization of Laccase Enzyme Production by Amesia atrobrunnea A2: A First Report

2020 ◽  
Vol 17 (01) ◽  
pp. 65-72
Author(s):  
A.T. Thakkar ◽  
D.C. Pandya ◽  
S.A. Bhatt
Keyword(s):  
Enzyme Production ◽  
First Report ◽  
Laccase Enzyme

scholarly journals Genomics Analysis and Degradation Characteristics of Lignin by Streptomyces Thermocarboxydus Strain DF3-3

2021 ◽  
Author(s):  
Fangyun Tan ◽  
Jun Cheng ◽  
Yu Zhang ◽  
Xingfu Jiang ◽  
Yueqiu Liu
Keyword(s):  
Energy Conversion ◽  
Manganese Peroxidase ◽  
Metabolic Pathways ◽  
Lignin Degradation ◽  
Degradation Products ◽  
Degradation Mechanism ◽  
Biomass Energy ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Degradation Intermediates

Abstract Background: Lignocellulose is an important raw material for biomass-to-energy conversion, and it exhibits a complex but inefficient degradation mechanism. Microbial degradation is promising due to its environmental adaptability and biochemical versatility, but the pathways used by microbes for lignin degradation have not been fully studied. Degradation intermediates and complex metabolic pathways require more study.Results: A novel actinomycete DF3-3, with the potential for lignin degradation, was screened and isolated. After morphological and molecular identification, DF3-3 was determined to be Streptomyces thermocarboxydus. The degradation of alkali lignin reached 31% within 15 days. Manganese peroxidase and laccase demonstrated their greatest activity levels, 1821.66 UL-1 and 1265.58 UL-1, respectively, on the sixth day. The highest lignin peroxidase activity was 480.33 UL-1 on the fourth day. A total of 19 lignin degradation intermediates were identified by gas chromatography-mass spectrometry (GC-MS), including 10 aromatic compounds. Genome sequencing and annotation identified 107 lignin-degrading enzyme-coding genes containing three core enzymatic systems for lignin depolymerization: laccases, peroxidases and manganese peroxidase. In total, 7 lignin metabolic pathways were predicted.Conclusions: Streptomyces thermocarboxydus strain DF3-3 has good lignin degradation ability. Degradation products and genomics analyses of DF3-3 show that it has a relatively complete lignin degradation pathway, including the β-ketoadipate pathway and peripheral reactions; gentisate pathway; anthranilate pathway; homogentisic pathway; and catabolic pathway for resorcinol. Two other pathways, the phenylacetate-CoA pathway and the 2,3-dihydroxyphenylpropionic acid pathway, are predicted based on genome data alone. This study provides the basis for future characterization of potential biotransformation enzyme systems for biomass energy conversion.

A fossil Aspergillus from Eocene Dominican amber

1988 ◽  
Vol 62 (01) ◽  
pp. 141-143 ◽  
Author(s):  
Gerard M. Thomas ◽  
George O. Poinar
Keyword(s):  
Dominican Republic ◽  
Fossil Species ◽  
First Report ◽  
Dominican Amber ◽  
Eocene Amber

A sporulating Aspergillus is described from a piece of Eocene amber originating from the Dominican Republic. The Aspergillus most closely resembles a form of the white spored phase of Aspergillus janus Raper and Thom. This is the first report of a fossil species of Aspergillus.

1392: First Report of a New Transurethral Detaching Prostatectomy of the Prostate (TUDP)

2005 ◽  
Vol 173 (4S) ◽  
pp. 377-378
Author(s):  
Yasunori Hiraoka ◽  
Kazuhiko Yamada ◽  
Yuji Shimizu ◽  
Hiroyuki Abe
Keyword(s):  
First Report
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